C57BL/6JCya-Kcnk13em1/Cya
Common Name:
Kcnk13-KO
Product ID:
S-KO-05344
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kcnk13-KO
Strain ID
KOCMP-217826-Kcnk13-B6J-VA
Gene Name
Product ID
S-KO-05344
Gene Alias
F730021E22Rik; Gm1570; Gm1685
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kcnk13em1/Cya mice (Catalog S-KO-05344) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049788
NCBI RefSeq
NM_001164427
Target Region
Exon 3
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Kcnk13, also known as THIK-1, codes for a potassium (K⁺) ion channel that is part of the two-pore potassium channel family. This channel plays essential roles in maintaining the resting membrane potential and ion homeostasis. It is involved in multiple biological processes such as microglial immune surveillance, cytokine release, and has connections to the NLRP3-inflammasome pathway [4,5]. Kcnk13 is specifically expressed in human microglia, and its elevated expression has been observed in post-mortem human brain tissue from patients with Alzheimer's disease [2].
In rats, knockdown of Kcnk13 using siRNA via a recording micropipette reduced ethanol-induced excitation of ventral tegmental area (VTA) neurons, and real-time PCR showed that its expression was altered in a time-dependent manner after alcohol withdrawal. In mice, knockdown of Kcnk13 with shRNA led to a selective reduction in ethanol-induced excitation of VTA neurons and increased alcohol drinking, suggesting that ethanol may stimulate VTA neurons by inhibiting KCNK13 [1,3].
In conclusion, Kcnk13 is crucial for the regulation of neuronal activity in the VTA, especially in the context of ethanol-induced excitation, and may play a role in the development of alcohol-related disorders. In addition, its up-regulation in Alzheimer's disease indicates a potential role in neurodegenerative diseases, highlighting the importance of Kcnk13-related research in understanding these disease mechanisms [1,3,5].
References:
1. You, Chang, Vandegrift, Bertha J, Brodie, Mark S. 2021. KCNK13 potassium channels in the ventral tegmental area of rats are important for excitation of ventral tegmental area neurons by ethanol. In Alcoholism, clinical and experimental research, 45, 1348-1358. doi:10.1111/acer.14630. https://pubmed.ncbi.nlm.nih.gov/33960499/
2. Bürli, Roland W, Doyle, Kevin J, Dickson, Louise, Carlton, Mark, Dawson, Lee A. 2024. Discovery of CVN293, a Brain Permeable KCNK13 (THIK-1) Inhibitor Suitable for Clinical Assessment. In ACS medicinal chemistry letters, 15, 646-652. doi:10.1021/acsmedchemlett.4c00035. https://pubmed.ncbi.nlm.nih.gov/38746889/
3. You, Chang, Savarese, Antonia, Vandegrift, Bertha J, Lasek, Amy W, Brodie, Mark S. 2018. Ethanol acts on KCNK13 potassium channels in the ventral tegmental area to increase firing rate and modulate binge-like drinking. In Neuropharmacology, 144, 29-36. doi:10.1016/j.neuropharm.2018.10.008. https://pubmed.ncbi.nlm.nih.gov/30332606/
4. Madry, Christian, Kyrargyri, Vasiliki, Arancibia-Cárcamo, I Lorena, Bryan, Robert M, Attwell, David. 2017. Microglial Ramification, Surveillance, and Interleukin-1β Release Are Regulated by the Two-Pore Domain K+ Channel THIK-1. In Neuron, 97, 299-312.e6. doi:10.1016/j.neuron.2017.12.002. https://pubmed.ncbi.nlm.nih.gov/29290552/
5. Tang, Hao, Sun, Yuhong, Fachim, Helene A, Reynolds, Gavin P, Harte, Michael K. . Elevated Expression of Two Pore Potassium Channel THIK-1 in Alzheimer's Disease: An Inflammatory Mechanism. In Journal of Alzheimer's disease : JAD, 95, 1757-1769. doi:10.3233/JAD-230616. https://pubmed.ncbi.nlm.nih.gov/37718820/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen