C57BL/6JCya-Cerkem1/Cya
Common Name:
Cerk-KO
Product ID:
S-KO-05738
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cerk-KO
Strain ID
KOCMP-223753-Cerk-B6J-VA
Gene Name
Product ID
S-KO-05738
Gene Alias
D330016D08Rik; mKIAA1646
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cerkem1/Cya mice (Catalog S-KO-05738) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044332
NCBI RefSeq
NM_145475
Target Region
Exon 6
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Ceramide kinase (CERK) is a mammalian lipid kinase responsible for phosphorylating ceramide to produce ceramide-1-phosphate (C1P) [3,4]. C1P is involved in membrane biology, regulation of membrane-bound proteins, and acts as a signaling entity. The CERK-C1P axis is part of the sphingolipid pathway, which is crucial for various cellular functions such as cell signaling, metabolism, growth, and death [4]. Genetic models, like gene knockout mouse models, are valuable for studying CERK.
In a CERK-knockout mouse model, inhibition of CERK exacerbated high-altitude pulmonary edema (HAPE) induced by a hypobaric hypoxic environment. CERK-derived C1P protected against HAPE by stabilizing circadian rhythms and maintaining mitochondrial dynamics. Specifically, CERK inhibition induced aryl hydrocarbon receptor nuclear translocator-like (ARNTL) autophagic degradation, triggering mitochondrial damage, while exogenous C1P prevented ARNTL degradation and alleviated HAPE [1]. In mutant KRAS non-small cell lung cancer (NSCLC), inhibition of CERK induced ferroptosis, reduced cell survival, and synergized with cisplatin in reducing in vivo tumor growth by dysregulating VDAC-mediated mitochondria function [2].
In conclusion, CERK, through the production of C1P, plays essential roles in maintaining mitochondrial dynamics, circadian rhythms, and redox homeostasis. CERK-KO mouse models have revealed its significance in diseases such as HAPE and NSCLC, highlighting its potential as a therapeutic target for these and potentially other related disease conditions.
References:
1. Tian, Liuyang, Zhao, Chenghui, Yan, Yan, He, Kunlun, Zhao, Xiaojing. 2023. Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics. In Journal of advanced research, 60, 75-92. doi:10.1016/j.jare.2023.07.008. https://pubmed.ncbi.nlm.nih.gov/37479181/
2. Vu, Ngoc T, Kim, Minjung, Stephenson, Daniel J, MacKnight, H Patrick, Chalfant, Charles E. . Ceramide Kinase Inhibition Drives Ferroptosis and Sensitivity to Cisplatin in Mutant KRAS Lung Cancer by Dysregulating VDAC-Mediated Mitochondria Function. In Molecular cancer research : MCR, 20, 1429-1442. doi:10.1158/1541-7786.MCR-22-0085. https://pubmed.ncbi.nlm.nih.gov/35560154/
3. Dong, Wei, Li, Qing, Lu, Xing, Zhang, Chong, Jin, Junfei. 2024. Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2. In Experimental & molecular medicine, 56, 946-958. doi:10.1038/s12276-024-01203-4. https://pubmed.ncbi.nlm.nih.gov/38556546/
4. Bornancin, Frédéric. 2010. Ceramide kinase: the first decade. In Cellular signalling, 23, 999-1008. doi:10.1016/j.cellsig.2010.11.012. https://pubmed.ncbi.nlm.nih.gov/21111813/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen