C57BL/6NCya-Ubash3aem1/Cya
Common Name:
Ubash3a-KO
Product ID:
S-KO-09429
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ubash3a-KO
Strain ID
KOCMP-328795-Ubash3a-B6N-VA
Gene Name
Product ID
S-KO-09429
Gene Alias
5830413C03Rik; C330001M22; Sts-2; TULA; TULA-1
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ubash3aem1/Cya mice (Catalog S-KO-09429) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000236745
NCBI RefSeq
NM_177823
Target Region
Exon 2~5
Size of Effective Region
~8.4 kb
Detailed Document
Overview of Gene Research
Ubash3a, also known as Ubiquitin Associated and SH3 Domain Containing A, is a negative regulator of T cell function. It plays a crucial role in autoimmunity and is associated with pathways regulating TCR-CD3 complex synthesis and turnover, including endoplasmic reticulum-associated protein degradation, endocytosis, etc. Genetic models, like gene knockout in mice, have been valuable in studying its function [1].
In NOD mice, Ubash3a-deficient animals showed accelerated type 1 diabetes development, with more advanced insulitis, increased accumulation of β-cell autoreactive T cells, and promoted salivary gland inflammation in females. Adoptive transfer experiments indicated that Ubash3a deficiency in T cells alone was sufficient to promote T1D development [2].
In conclusion, Ubash3a is essential in regulating T cell-related functions and autoimmunity. Gene knockout mouse models, such as those in NOD mice, have been instrumental in revealing its role in type 1 diabetes and other autoimmune-related conditions, enhancing our understanding of the underlying molecular mechanisms and potential disease-associated pathways.
References:
1. Ge, Yan, Paisie, Taylor K, Chen, Sixue, Concannon, Patrick. 2019. UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes. In Journal of immunology (Baltimore, Md. : 1950), 203, 2827-2836. doi:10.4049/jimmunol.1801338. https://pubmed.ncbi.nlm.nih.gov/31659016/
2. Chen, Yi-Guang, Ciecko, Ashley E, Khaja, Shamim, Geurts, Aron M, Lieberman, Scott M. 2020. UBASH3A deficiency accelerates type 1 diabetes development and enhances salivary gland inflammation in NOD mice. In Scientific reports, 10, 12019. doi:10.1038/s41598-020-68956-6. https://pubmed.ncbi.nlm.nih.gov/32694640/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen