C57BL/6JCya-Rbm33em1/Cya
Common Name
Rbm33-KO
Product ID
S-KO-09785
Backgroud
C57BL/6JCya
Strain ID
KOCMP-381626-Rbm33-B6J-VA
When using this mouse strain in a publication, please cite “Rbm33-KO Mouse (Catalog S-KO-09785) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Rbm33-KO
Strain ID
KOCMP-381626-Rbm33-B6J-VA
Gene Name
Product ID
S-KO-09785
Gene Alias
3200001K10Rik, 6430512A10Rik, Prr8
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000059644
NCBI RefSeq
NM_028234
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Overview of Gene Research
Rbm33, an RNA-binding protein, has multiple essential functions. It is involved in regulating mRNA m6A demethylation by forming a complex with ALKBH5, which impacts the tumorigenesis of head-neck squamous cell carcinoma (HNSCC) [1]. It also directs the nuclear export of transcripts containing GC-rich elements, recruiting components of the TREX-NXF1/NXT1 RNA export pathway [2].
In a study on hippocampus aging, hippocampus-specific genetic deletion of Rbm33 alleviated age-related declines in learning and memory in aged Rbm33 knockout (Rbm33-/-) mice, while hippocampus-specific overexpression exacerbated these declines. Mechanistically, Rbm33 forms liquid-liquid phase separation (LLPS) and binds to the CDKN1A (p21cip1) promoter to induce microglial senescence, facilitating hippocampus aging [3].
In conclusion, Rbm33 plays crucial roles in mRNA m6A demethylation-related tumorigenesis, nuclear export of specific transcripts, and hippocampus aging. The use of Rbm33 knockout mouse models has revealed its functions in age-related cognitive decline, highlighting its potential as a therapeutic target for neurodegenerative diseases associated with hippocampal aging [3].
References:
1. Yu, Fang, Zhu, Allen C, Liu, Shun, He, Chuan, Qian, Zhijian. 2023. RBM33 is a unique m6A RNA-binding protein that regulates ALKBH5 demethylase activity and substrate selectivity. In Molecular cell, 83, 2003-2019.e6. doi:10.1016/j.molcel.2023.05.010. https://pubmed.ncbi.nlm.nih.gov/37257451/
2. Thomas, Anu, Rehfeld, Frederick, Zhang, He, Gillet, Frank, Mendell, Joshua T. 2022. RBM33 directs the nuclear export of transcripts containing GC-rich elements. In Genes & development, 36, 550-565. doi:10.1101/gad.349456.122. https://pubmed.ncbi.nlm.nih.gov/35589130/
3. Yang, Xiaowen, Wei, Lin, Zhong, Saifeng, Zhang, Yonggang, Yu, Aiqing. 2025. Liquid-liquid phase separation of RBM33 facilitates hippocampus aging by inducing microglial senescence by activating CDKN1A. In International journal of biological macromolecules, 310, 142986. doi:10.1016/j.ijbiomac.2025.142986. https://pubmed.ncbi.nlm.nih.gov/40216137/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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