Ncbp1, also known as Nuclear cap-binding protein 1, is essential for capped RNA processing and intracellular localization. It forms the nuclear Cap-Binding Complex (CBC) with NCBP2 and an alternative CBC with NCBP3, playing roles in RNA fate decisions, such as transcription, splicing, polyadenylation, and mRNA export [5,6,8]. It is also involved in translation as part of the nuclear cap-binding protein complex-dependent translation machinery [9].

In disease-related research, NCBP1 shows diverse impacts. In diffuse large B-cell lymphoma (DLBCL), its elevated expression is associated with poor prognosis, and it promotes cell proliferation via the METTL3-mediated m6A modification of c-Myc [1]. In lung adenocarcinoma, NCBP1 overexpression promotes cancer cell growth, migration, etc., by up-regulating CUL4B [3]. In breast cancer, NCBP1 is identified as a prognostic and immune-associated biomarker, and knockdown of it suppresses malignancy in vitro [7]. In renal tubular epithelial cells, the IGF2BP3/NCBP1 complex inhibits senescence by regulating CDK6 mRNA stability [2]. Conversely, in epilepsy, NCBP1 is downregulated in the epileptic hippocampus, and its overexpression improves cognitive function by reducing oxidative stress, neuronal loss, and glial activation [4].

In conclusion, Ncbp1 is crucial for RNA-related processes and is implicated in multiple diseases. Studies on Ncbp1, including those using in vitro knockdown models, have provided insights into its roles in cancer, renal senescence, and epilepsy, potentially guiding the development of targeted therapies for these conditions.

References:

1. Meng, Sibo, Xia, Yuan, Li, Mingying, Sun, Tao, Ji, Chunyan. 2023. NCBP1 enhanced proliferation of DLBCL cells via METTL3-mediated m6A modification of c-Myc. In Scientific reports, 13, 8606. doi:10.1038/s41598-023-35777-2. https://pubmed.ncbi.nlm.nih.gov/37244946/

2. Li, Yaqin, Luo, Congwei, Cai, Yating, Wang, Hongsheng, Niu, Hongxin. 2024. IGF2BP3/NCBP1 complex inhibits renal tubular senescence through regulation of CDK6 mRNA stability. In Translational research : the journal of laboratory and clinical medicine, 273, 1-15. doi:10.1016/j.trsl.2024.06.004. https://pubmed.ncbi.nlm.nih.gov/38945255/

3. Zhang, Huijun, Wang, An, Tan, Yulong, Chen, Xiaofeng, He, Zelai. 2019. NCBP1 promotes the development of lung adenocarcinoma through up-regulation of CUL4B. In Journal of cellular and molecular medicine, 23, 6965-6977. doi:10.1111/jcmm.14581. https://pubmed.ncbi.nlm.nih.gov/31448526/

4. Gao, Xiaoying, You, Zhipeng, Huang, Cong, Qi, Sihua, Sun, Jiahang. 2023. NCBP1 Improves Cognitive Function in Mice by Reducing Oxidative Stress, Neuronal Loss, and Glial Activation After Status Epilepticus. In Molecular neurobiology, 60, 6676-6688. doi:10.1007/s12035-023-03497-3. https://pubmed.ncbi.nlm.nih.gov/37474884/

5. Dou, Yuhui, Barbosa, Isabelle, Jiang, Hua, LaCava, John, Jensen, Torben Heick. . NCBP3 positively impacts mRNA biogenesis. In Nucleic acids research, 48, 10413-10427. doi:10.1093/nar/gkaa744. https://pubmed.ncbi.nlm.nih.gov/32960271/

6. Gebhardt, Anna, Habjan, Matthias, Benda, Christian, Habermann, Bianca, Pichlmair, Andreas. 2015. mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3. In Nature communications, 6, 8192. doi:10.1038/ncomms9192. https://pubmed.ncbi.nlm.nih.gov/26382858/

7. Li, Jianrong, Zheng, Lin, Song, Liying, Bai, Wenqi, Qi, Liqiang. 2023. Identification and validation of N7 -methylguanosine-associated gene NCBP1 as prognostic and immune-associated biomarkers in breast cancer patients. In Journal of cellular and molecular medicine, 28, e18067. doi:10.1111/jcmm.18067. https://pubmed.ncbi.nlm.nih.gov/38071502/

8. Clarke, Bradley P, Angelos, Alexia E, Mei, Menghan, Xie, Yihu, Ren, Yi. 2024. Cryo-EM structure of the CBC-ALYREF complex. In eLife, 12, . doi:10.7554/eLife.91432. https://pubmed.ncbi.nlm.nih.gov/39282949/

9. Park, Yeonkyoung, Park, Joori, Kim, Yoon Ki. 2017. Crosstalk between translation and the aggresome-autophagy pathway. In Autophagy, 14, 1079-1081. doi:10.1080/15548627.2017.1358849. https://pubmed.ncbi.nlm.nih.gov/28837386/