C57BL/6NCya-Gsdmdem1/Cya
Common Name
Gsdmd-KO
Product ID
S-KO-12963
Backgroud
C57BL/6NCya
Strain ID
KOCMP-69146-Gsdmd-B6N-VA
When using this mouse strain in a publication, please cite “Gsdmd-KO Mouse (Catalog S-KO-12963) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gsdmd-KO
Strain ID
KOCMP-69146-Gsdmd-B6N-VA
Gene Name
Product ID
S-KO-12963
Gene Alias
1810036L03Rik, DF5L, Dfna5l, GsdmD-1, Gsdmdc1, M2-4
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000023238
NCBI RefSeq
NM_026960
Target Region
Exon 2~11
Size of Effective Region
~4.3 kb
Overview of Gene Research
Gasdermin D (Gsdmd) is a key executor in pyroptosis, a form of programmed cell death. Inflammatory caspases, such as caspase-1,-4,-5 and-11, cleave Gsdmd, which is essential for pyroptosis induction. This process is involved in innate defenses, and Gsdmd-related pyroptosis has significance in host defense, inflammatory and autoimmune diseases [1,3].
Genetic KO of Gsdmd in mice alleviated depression-like behaviors and inhibited pyroptosis-associated protein expression, indicating its role in astrocyte pathological injury in depression [2]. In obstructive nephropathy, Gsdmd deletion in neutrophils protected the kidney from fibrosis as Gsdmd-dependent neutrophil extracellular traps promote macrophage-to-myofibroblast transition and renal fibrosis [4].
In conclusion, Gsdmd is crucial in pyroptosis, playing significant roles in diseases like depression and renal fibrosis. The use of Gsdmd KO mouse models has been instrumental in revealing its functions in these specific disease conditions, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Shi, Jianjin, Zhao, Yue, Wang, Kun, Wang, Fengchao, Shao, Feng. 2015. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. In Nature, 526, 660-5. doi:10.1038/nature15514. https://pubmed.ncbi.nlm.nih.gov/26375003/
2. Li, Shanshan, Sun, Yiming, Song, Mengmeng, Lu, Ming, Hu, Gang. 2021. NLRP3/caspase-1/GSDMD-mediated pyroptosis exerts a crucial role in astrocyte pathological injury in mouse model of depression. In JCI insight, 6, . doi:10.1172/jci.insight.146852. https://pubmed.ncbi.nlm.nih.gov/34877938/
3. Li, Zihao, Ji, Senlin, Jiang, Mei-Ling, Xu, Yun, Zhang, Cun-Jin. 2022. The Regulation and Modification of GSDMD Signaling in Diseases. In Frontiers in immunology, 13, 893912. doi:10.3389/fimmu.2022.893912. https://pubmed.ncbi.nlm.nih.gov/35774778/
4. Wang, Yujia, Li, Yinshuang, Chen, Zhimin, Chen, Hong, Xu, Yanfang. 2022. GSDMD-dependent neutrophil extracellular traps promote macrophage-to-myofibroblast transition and renal fibrosis in obstructive nephropathy. In Cell death & disease, 13, 693. doi:10.1038/s41419-022-05138-4. https://pubmed.ncbi.nlm.nih.gov/35941120/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Publications
Cellular & Molecular Immunology
2024-10-08
The TET3 inflammasome senses unique long HSV-1 proteins for virus particle budding from the nucleus
Read More
Cell Death Discovery
2024-06-21
CCL11 released by GSDMD-mediated macrophage pyroptosis regulates angiogenesis after hindlimb ischemia
Read More
Nature Cell Biology
2024-03-27
A palmitoylation–depalmitoylation relay spatiotemporally controls GSDMD activation in pyroptosis
Read More
Immunity
2023-04-01
The orphan receptor Nur77 binds cytoplasmic LPS to activate the non-canonical NLRP3 inflammasome
Read More
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