C57BL/6JCya-Plce1em1/Cya
Common Name
Plce1-KO
Product ID
S-KO-14268
Backgroud
C57BL/6JCya
Strain ID
KOCMP-74055-Plce1-B6J-VA
When using this mouse strain in a publication, please cite “Plce1-KO Mouse (Catalog S-KO-14268) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Plce1-KO
Strain ID
KOCMP-74055-Plce1-B6J-VA
Gene Name
Product ID
S-KO-14268
Gene Alias
4933403A21Rik, PLCepsilon, Plce, mKIAA1516
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 19
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000169713
NCBI RefSeq
NM_019588
Target Region
Exon 2
Size of Effective Region
~0.3 kb
Overview of Gene Research
PLCE1, or phospholipase C epsilon 1, is a member of the phospholipase C (PLC) family, controlling phosphoinositide levels in cells. It is involved in multiple biological processes such as cell growth, differentiation, and is associated with pathways like PKCα-E2F1-MCM7 and phosphoinositide-mediated cell death regulation. It has been identified as a susceptibility gene in several diseases, highlighting its biological importance [1,2].
In esophageal squamous cell carcinoma (ESCC), PLCE1 promotes tumor progression. It activates PKCα-mediated phosphorylation of E2F1, upregulating MCM7 and miR-106b-5p expression, and potentiates MCM7 phosphorylation by RIOK2 [1]. In doxorubicin-induced cardiotoxicity, PLCE1 enhances mitochondrial dysfunction to promote GSDME-mediated pyroptosis, while its deletion ameliorates mitochondrial dysfunction and reduces pyroptotic cell death [2]. In podocytes, PLCE1 knockout leads to decreased Rho GTPase activity, reduced cell migration, and affects cell proliferation and differentiation [3].
In summary, PLCE1 plays crucial roles in multiple biological processes and disease conditions. Studies on PLCE1 using gene knockout models have provided insights into its functions in cancer, cardiotoxicity, and nephrotic syndrome-related processes, enhancing our understanding of disease mechanisms and potentially paving the way for new therapeutic strategies.
References:
1. Shi, Qi, Xu, Guixuan, Jiang, Yuliang, Cui, Xiaobin, Li, Feng. . Phospholipase PLCE1 Promotes Transcription and Phosphorylation of MCM7 to Drive Tumor Progression in Esophageal Cancer. In Cancer research, 84, 560-576. doi:10.1158/0008-5472.CAN-23-1633. https://pubmed.ncbi.nlm.nih.gov/38117512/
2. Tuersuntuoheti, Maierhaba, Peng, Fei, Li, Juexing, Gao, Hailan, Gong, Hui. 2024. PLCE1 enhances mitochondrial dysfunction to promote GSDME-mediated pyroptosis in doxorubicin-induced cardiotoxicity. In Biochemical pharmacology, 223, 116142. doi:10.1016/j.bcp.2024.116142. https://pubmed.ncbi.nlm.nih.gov/38499110/
3. Yu, Seyoung, Choi, Won-Il, Choi, Yo Jun, Hildebrandt, Friedhelm, Gee, Heon Yung. 2020. PLCE1 regulates the migration, proliferation, and differentiation of podocytes. In Experimental & molecular medicine, 52, 594-603. doi:10.1038/s12276-020-0410-4. https://pubmed.ncbi.nlm.nih.gov/32238860/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.