C57BL/6JCya-Clec2dem1/Cya
Common Name
Clec2d-KO
Product ID
S-KO-15453
Backgroud
C57BL/6JCya
Strain ID
KOCMP-93694-Clec2d-B6J-VB
When using this mouse strain in a publication, please cite “Clec2d-KO Mouse (Catalog S-KO-15453) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Clec2d-KO
Strain ID
KOCMP-93694-Clec2d-B6J-VB
Gene Name
Product ID
S-KO-15453
Gene Alias
Clr-b, Clrb, Ocil
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000032260
NCBI RefSeq
NM_053109
Target Region
Exon 2~4
Size of Effective Region
~1.8 kb
Overview of Gene Research
Clec2d, also known as Lectin-like transcript 1 (LLT1), is a C-type lectin receptor. It plays diverse roles in immune-related processes. It is involved in dimerization with other receptors like TLR2, and this dimerization modulates immune responses. Also, it has a role in sensing cell death, and is associated with immune-evasion mechanisms in tumors through its interaction with CD161 [1-3].
In Clec2d-deficient female mice, they are resistant to Candida albicans infection due to increased IL-12 production and subsequent generation of IFN-γ-producing NK cells. This indicates that Clec2d negatively regulates antifungal immunity by suppressing IRF5-mediated IL-12 production [1]. In the context of cancer, targeting the CD161-Clec2d interaction with monoclonal antibodies enhanced T-cell-mediated immunity against hematological malignancies in humanized mouse models, and pan-cancer analysis shows that the CLEC2D/KLRB1 ratio is higher in most cancer types and increases with advancing pathological stages [2,3].
In conclusion, Clec2d is a crucial player in regulating immune responses, especially in antifungal immunity and cancer immune-evasion. The use of gene-knockout mouse models has revealed its negative regulatory role in antifungal immunity and its potential as a target for cancer immunotherapy. These findings contribute to a better understanding of the underlying mechanisms of related diseases and provide potential therapeutic strategies.
References:
1. Li, Fan, Wang, Hui, Li, Yan-Qi, Gu, Yebo, Jia, Xin-Ming. 2023. C-type lectin receptor 2d forms homodimers and heterodimers with TLR2 to negatively regulate IRF5-mediated antifungal immunity. In Nature communications, 14, 6718. doi:10.1038/s41467-023-42216-3. https://pubmed.ncbi.nlm.nih.gov/37872182/
2. Alvarez Calderon, Francesca, Kang, Byong H, Kyrysyuk, Oleksandr, Wittrup, K Dane, Wucherpfennig, Kai W. . Targeting of the CD161 inhibitory receptor enhances T-cell-mediated immunity against hematological malignancies. In Blood, 143, 1124-1138. doi:10.1182/blood.2023022882. https://pubmed.ncbi.nlm.nih.gov/38153903/
3. Zhu, Yaoyao, Zhang, Huajie, Shao, Ruoyang, Lu, Peiyuan, Ma, Zhongrui. 2024. Comprehensive pan-cancer analysis of KLRB1-CLEC2D pair and identification of small molecule inhibitors to disrupt their interaction. In International immunopharmacology, 140, 112908. doi:10.1016/j.intimp.2024.112908. https://pubmed.ncbi.nlm.nih.gov/39133960/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.