C57BL/6JCya-Clec2dem1/Cya
Common Name
Clec2d-KO
Product ID
S-KO-15453
Backgroud
C57BL/6JCya
Strain ID
KOCMP-93694-Clec2d-B6J-VB
Status
When using this mouse strain in a publication, please cite “Clec2d-KO Mouse (Catalog S-KO-15453) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Clec2d-KO
Strain ID
KOCMP-93694-Clec2d-B6J-VB
Gene Name
Product ID
S-KO-15453
Gene Alias
Clrb, Ocil, Clr-b
Background
C57BL/6JCya
Gene Full Name
C-type lectin domain family 2, member d
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 6 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000032260
NCBI Transcript ID
NM_053109
Target Region
Exon 2~4
Size of Effective Region
~1.8 kb
Overview of Gene Research
Clec2d, also known as Lectin-like transcript 1 (LLT1), is a C-type lectin receptor. It plays diverse roles in immune-related processes. It is involved in dimerization with other receptors like TLR2, and this dimerization modulates immune responses. Also, it has a role in sensing cell death, and is associated with immune-evasion mechanisms in tumors through its interaction with CD161 [1-3].
In Clec2d-deficient female mice, they are resistant to Candida albicans infection due to increased IL-12 production and subsequent generation of IFN-γ-producing NK cells. This indicates that Clec2d negatively regulates antifungal immunity by suppressing IRF5-mediated IL-12 production [1]. In the context of cancer, targeting the CD161-Clec2d interaction with monoclonal antibodies enhanced T-cell-mediated immunity against hematological malignancies in humanized mouse models, and pan-cancer analysis shows that the CLEC2D/KLRB1 ratio is higher in most cancer types and increases with advancing pathological stages [2,3].
In conclusion, Clec2d is a crucial player in regulating immune responses, especially in antifungal immunity and cancer immune-evasion. The use of gene-knockout mouse models has revealed its negative regulatory role in antifungal immunity and its potential as a target for cancer immunotherapy. These findings contribute to a better understanding of the underlying mechanisms of related diseases and provide potential therapeutic strategies.
References:
1. Li, Fan, Wang, Hui, Li, Yan-Qi, Gu, Yebo, Jia, Xin-Ming. 2023. C-type lectin receptor 2d forms homodimers and heterodimers with TLR2 to negatively regulate IRF5-mediated antifungal immunity. In Nature communications, 14, 6718. doi:10.1038/s41467-023-42216-3. https://pubmed.ncbi.nlm.nih.gov/37872182/
2. Alvarez Calderon, Francesca, Kang, Byong H, Kyrysyuk, Oleksandr, Wittrup, K Dane, Wucherpfennig, Kai W. . Targeting of the CD161 inhibitory receptor enhances T-cell-mediated immunity against hematological malignancies. In Blood, 143, 1124-1138. doi:10.1182/blood.2023022882. https://pubmed.ncbi.nlm.nih.gov/38153903/
3. Zhu, Yaoyao, Zhang, Huajie, Shao, Ruoyang, Lu, Peiyuan, Ma, Zhongrui. 2024. Comprehensive pan-cancer analysis of KLRB1-CLEC2D pair and identification of small molecule inhibitors to disrupt their interaction. In International immunopharmacology, 140, 112908. doi:10.1016/j.intimp.2024.112908. https://pubmed.ncbi.nlm.nih.gov/39133960/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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