C57BL/6JCya-Iappem1/Cya
Common Name:
Iapp-KO
Product ID:
S-KO-16503
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Iapp-KO
Strain ID
KOCMP-15874-Iapp-B6J-VB
Gene Name
Product ID
S-KO-16503
Gene Alias
DAP
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Iappem1/Cya mice (Catalog S-KO-16503) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041993
NCBI RefSeq
NM_010491.2
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Iapp, also known as islet amyloid polypeptide or amylin, is a 37-amino-acid peptide hormone produced by pancreatic β-cells. It activates specific multisubunit G protein-coupled receptors and is a key glucoregulatory hormone, playing an important role in energy metabolism. It also has other reported actions on systems like the cardiovascular system and bone, and functionally interacts with other hormones in the central nervous system [2].
In type 2 diabetes (T2DM), Iapp forms cytotoxic oligomers and amyloid fibrils in islets, which contribute to β-cell dysfunction, death, and islet inflammation [1,3,5]. In transgenic mice overexpressing human Iapp (hIapp), intracellular pro-apoptotic signals can be generated, and extracellular amyloidosis is directly related to β-cell apoptosis in T2DM [1]. In mice, overexpression of soluble rodent or oligomer-prone human Iapp induced changes in the islet transcriptome similar to those in prediabetes and T2DM in humans, including decreased expression of genes conferring β-cell identity and induction of islet inflammation in the case of human Iapp overexpression [4].
In conclusion, Iapp is a crucial pancreatic hormone in glucose and energy metabolism. Its abnormal aggregation in models like transgenic mice reveals its significant role in the pathogenesis of T2DM, contributing to β-cell injury, islet inflammation, and dedifferentiation. Understanding Iapp through these model-based studies provides insights into T2DM mechanisms and potential therapeutic targets [1,4].
References:
1. Raleigh, Daniel, Zhang, Xiaoxue, Hastoy, Benoît, Clark, Anne. 2017. The β-cell assassin: IAPP cytotoxicity. In Journal of molecular endocrinology, 59, R121-R140. doi:10.1530/JME-17-0105. https://pubmed.ncbi.nlm.nih.gov/28811318/
2. Hay, Debbie L, Chen, Steve, Lutz, Thomas A, Parkes, David G, Roth, Jonathan D. . Amylin: Pharmacology, Physiology, and Clinical Potential. In Pharmacological reviews, 67, 564-600. doi:10.1124/pr.115.010629. https://pubmed.ncbi.nlm.nih.gov/26071095/
3. Fernández, Marta S. 2014. Human IAPP amyloidogenic properties and pancreatic β-cell death. In Cell calcium, 56, 416-27. doi:10.1016/j.ceca.2014.08.011. https://pubmed.ncbi.nlm.nih.gov/25224501/
4. Blencowe, Montgomery, Furterer, Allison, Wang, Qing, Butler, Peter C, Gurlo, Tatyana. 2021. IAPP-induced beta cell stress recapitulates the islet transcriptome in type 2 diabetes. In Diabetologia, 65, 173-187. doi:10.1007/s00125-021-05569-2. https://pubmed.ncbi.nlm.nih.gov/34554282/
5. Montane, J, Klimek-Abercrombie, A, Potter, K J, Westwell-Roper, C, Bruce Verchere, C. . Metabolic stress, IAPP and islet amyloid. In Diabetes, obesity & metabolism, 14 Suppl 3, 68-77. doi:10.1111/j.1463-1326.2012.01657.x. https://pubmed.ncbi.nlm.nih.gov/22928566/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen