C57BL/6JCya-Il7rem1/Cya
Common Name:
Il7r-KO
Product ID:
S-KO-17359
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Il7r-KO
Strain ID
KOCMP-16197-Il7r-B6J-VA
Gene Name
Product ID
S-KO-17359
Gene Alias
CD127; IL-7Ralpha
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il7rem1/Cya mice (Catalog S-KO-17359) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003981
NCBI RefSeq
NM_008372
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Il7r, the gene encoding interleukin-7 receptor α chain (IL-7Rα), is a crucial component of the IL-7 receptor. IL-7R consists of IL-7Rα and γc chains, and is a member of the γc family of receptors for cytokines such as IL-2,-4,-9,-15, and-21. The IL-7/IL-7R signaling pathway functions through major signals via JAK1, JAK3 to STAT5, as well as non-canonical STAT3, STAT1, PI3K/AKT and MEK/ERK pathways. This pathway is essential for the development, homeostatic survival, and differentiation of lymphoid cells, including immature thymocytes, naïve T-cells, memory T-cells, pro-B-cells, and innate lymphocytes [2].
In disease research, in adult acute myeloid leukemia patients with t(8;21), low IL7R transcript level at diagnosis predicted relapse, potentially due to differences in T-cell quantity, status, and function [1]. In T-cell acute lymphoblastic leukemia, mutant IL7R alone can induce T-ALL with long-latency in transgenic zebrafish, and it collaborates with Myc to drive early-onset T-ALL, activating STAT5 and AKT pathways and enriching for leukemia-propagating cells [3]. In acute myeloid leukemia, IL7R mutations are associated with an inferior prognosis, including higher non-relapse mortality, shorter overall survival, and event-free survival, even in patients who have undergone hematopoietic stem cell transplantation [4].
In conclusion, Il7r is vital for lymphoid cell development and homeostasis through the IL-7/IL-7R signaling pathway. Research using genetic models like transgenic zebrafish and human patient-based genetic analysis has revealed its significant role in leukemia prognosis and pathogenesis. Understanding Il7r function provides insights into lymphoid-related biological processes and disease mechanisms, potentially guiding new therapeutic strategies for leukemia.
References:
1. Xu, Nan, Sun, Kai, Wang, Ya-Zhe, Huang, Xiao-Jun, Qin, Ya-Zhen. 2022. Low IL7R Expression at Diagnosis Predicted Relapse in Adult Acute Myeloid Leukemia Patients With t(8;21). In Frontiers in immunology, 13, 909104. doi:10.3389/fimmu.2022.909104. https://pubmed.ncbi.nlm.nih.gov/35874754/
2. Winer, Hila, Rodrigues, Gisele O L, Hixon, Julie A, Li, Wenqing, Durum, Scott K. 2022. IL-7: Comprehensive review. In Cytokine, 160, 156049. doi:10.1016/j.cyto.2022.156049. https://pubmed.ncbi.nlm.nih.gov/36201890/
3. Oliveira, Mariana L, Veloso, Alexandra, Garcia, Elaine G, Langenau, David M, Barata, João T. 2022. Mutant IL7R collaborates with MYC to induce T-cell acute lymphoblastic leukemia. In Leukemia, 36, 1533-1540. doi:10.1038/s41375-022-01590-5. https://pubmed.ncbi.nlm.nih.gov/35581375/
4. Tao, Qiqi, Wu, Qiaoyuan, Xue, Yutong, Liu, Qifa, Jin, Hua. 2024. Prognostic impact of IL7R mutations on acute myeloid leukemia. In Therapeutic advances in hematology, 15, 20406207241279533. doi:10.1177/20406207241279533. https://pubmed.ncbi.nlm.nih.gov/39346679/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen