C57BL/6JCya-Mchr1em1/Cya
Common Name:
Mchr1-KO
Product ID:
S-KO-17371
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mchr1-KO
Strain ID
KOCMP-207911-Mchr1-B6J-VB
Gene Name
Product ID
S-KO-17371
Gene Alias
Gpr24; Mch1r
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mchr1em1/Cya mice (Catalog S-KO-17371) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000166855
NCBI RefSeq
NM_145132.2
Target Region
Exon 2
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Mchr1, short for melanin-concentrating hormone receptor 1, is a G-protein-coupled receptor. It has a crucial role in regulating appetite and energy homeostasis, with its ligand being melanin-concentrating hormone (MCH) secreted mainly by the hypothalamus. It is also involved in pathways related to emotion regulation and has potential importance in metabolism-related biological processes [1,3]. Genetic models, such as gene knockout mice, can be valuable for studying Mchr1's functions.
In Mchr1-deficient mice, osteoporosis occurs due to a reduction in cortical bone mass, with increased serum levels of c-telopeptide indicating high bone turnover osteoporosis. This reveals that Mchr1-signalling is involved in tonic stimulation of bone mass [2]. In systemic sclerosis (SSc), Mchr1 promotes the fibrotic response in normal human dermal fibroblasts (NHDF) through modulating profibrotic factors, intracellular cAMP levels, and PDGF-BB-induced signalling pathways, suggesting it plays a role in SSc pathogenesis and could be a therapeutic target [4].
In conclusion, Mchr1 is essential in regulating energy homeostasis and appetite. The Mchr1-KO mouse models have shown its importance in bone mass regulation, highlighting its significance in osteoporosis-related research. Additionally, its role in SSc-associated fibrosis provides potential therapeutic implications for this autoimmune disease.
References:
1. Johansson, Anders. 2016. Evolution of physicochemical properties of melanin concentrating hormone receptor 1 (MCHr1) antagonists. In Bioorganic & medicinal chemistry letters, 26, 4559-4564. doi:10.1016/j.bmcl.2016.08.072. https://pubmed.ncbi.nlm.nih.gov/27595423/
2. Bohlooly-Y, Mohammad, Mahlapuu, Margit, Andersén, Harriet, Morgan, David G, Ohlsson, Claes. . Osteoporosis in MCHR1-deficient mice. In Biochemical and biophysical research communications, 318, 964-9. doi:. https://pubmed.ncbi.nlm.nih.gov/15147966/
3. Wang, Meng, Zhai, Yue, Lei, Xiaowei, Li, Qingfeng, Zhang, Chao. 2022. Determination of the Interaction and Pharmacological Modulation of MCHR1 Signaling by the C-Terminus of MRAP2 Protein. In Frontiers in endocrinology, 13, 848728. doi:10.3389/fendo.2022.848728. https://pubmed.ncbi.nlm.nih.gov/35311242/
4. Takamura, Naoko, Renaud, Ludivine, da Silveira, Willian Abraham, Feghali-Bostwick, Carol. 2021. PDGF Promotes Dermal Fibroblast Activation via a Novel Mechanism Mediated by Signaling Through MCHR1. In Frontiers in immunology, 12, 745308. doi:10.3389/fimmu.2021.745308. https://pubmed.ncbi.nlm.nih.gov/34912333/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen