C57BL/6JCya-Trim45em1/Cya
Common Name:
Trim45-KO
Product ID:
S-KO-17424
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Trim45-KO
Strain ID
KOCMP-229644-Trim45-B6J-VB
Gene Name
Product ID
S-KO-17424
Gene Alias
4921530N01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trim45em1/Cya mice (Catalog S-KO-17424) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000134993
NCBI RefSeq
NM_001165953
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
TRIM45, a member of the TRIM (Tripartite motif) protein family, is a ubiquitin E3 ligase. It contains a RING finger, two B-boxes, a coiled-coil domain, and a filamin-type immunoglobulin domain. TRIM45 is involved in various biological processes such as cell proliferation, differentiation, and development, and is associated with pathways like MAPK and NF-κB [7,8].
Knocking down TRIM45 protected against neuronal damage and cognitive impairment in septic mice, as it inhibited microglial pyroptosis and the secretion of inflammatory cytokines through the Atg5/NLRP3 axis [1]. In cerebral ischemia and reperfusion injury, microglia-specific knockdown of TRIM45 reduced infarct size, mitigated neurological deficit scores, and improved cognitive function by suppressing microglia-mediated neuroinflammation via the TRIM45-TAB2-NF-κB axis [2]. In non-alcoholic steatohepatitis (NASH)-progressed hepatocellular carcinoma (HCC), TRIM45 promoted fatty acid synthesis and accelerated the NASH-to-HCC transition by catalyzing FABP5 ubiquitylation [3]. In non-small cell lung cancer, overexpression of TRIM45 induced G1 arrest and promoted apoptosis [4]. In zebrafish embryos, knockdown of trim45 reduced the size of the diencephalon and eye [5]. In glioma, knockout of TRIM45 promoted cell proliferation and inhibited apoptosis [6]. In chicken cells, knockdown of TRIM45 enhanced ALV-J replication [9].
In conclusion, TRIM45 plays diverse roles in different biological processes and diseases. Gene-knockout and knockdown models in mice, zebrafish, and chicken cells have been crucial in revealing its functions. In diseases such as septic encephalopathy, cerebral ischemia, NASH-progressed HCC, non-small cell lung cancer, glioma, and ALV-J infection, TRIM45 either promotes or suppresses disease-related processes, highlighting its potential as a therapeutic target.
References:
1. Huang, Xuliang, Ye, Changzhou, Zhao, Xinyu, Zhang, Anqi, Mo, Yunchang. 2023. TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy. In Journal of neuroinflammation, 20, 284. doi:10.1186/s12974-023-02959-8. https://pubmed.ncbi.nlm.nih.gov/38037161/
2. Xia, Qian, Zhan, Gaofeng, Mao, Meng, Zhao, Yin, Li, Xing. 2022. TRIM45 causes neuronal damage by aggravating microglia-mediated neuroinflammation upon cerebral ischemia and reperfusion injury. In Experimental & molecular medicine, 54, 180-193. doi:10.1038/s12276-022-00734-y. https://pubmed.ncbi.nlm.nih.gov/35217833/
3. Li, Xiaomian, He, Wenzhi, Chen, Xi, Wang, Haitao, Yuan, Yufeng. 2024. TRIM45 facilitates NASH-progressed HCC by promoting fatty acid synthesis via catalyzing FABP5 ubiquitylation. In Oncogene, 43, 2063-2077. doi:10.1038/s41388-024-03056-7. https://pubmed.ncbi.nlm.nih.gov/38755308/
4. Peng, Xiyang, Wen, Yao, Zha, Lagabaiyila, Wu, Xiushan, Fan, Xiongwei. . TRIM45 Suppresses the Development of Non-small Cell Lung Cancer. In Current molecular medicine, 20, 299-306. doi:10.2174/1566524019666191017143833. https://pubmed.ncbi.nlm.nih.gov/31663468/
5. Choe, Seoyeon, Huh, Tae-Lin, Rhee, Myungchull. 2020. Trim45 is essential to the development of the diencephalon and eye in zebrafish embryos. In Animal cells and systems, 24, 99-106. doi:10.1080/19768354.2020.1751281. https://pubmed.ncbi.nlm.nih.gov/32489689/
6. Zhang, Jindong, Zhang, Chuanxia, Cui, Jun, Zhi, Feng, Wang, Rong-Fu. 2017. TRIM45 functions as a tumor suppressor in the brain via its E3 ligase activity by stabilizing p53 through K63-linked ubiquitination. In Cell death & disease, 8, e2831. doi:10.1038/cddis.2017.149. https://pubmed.ncbi.nlm.nih.gov/28542145/
7. Wang, Yuequn, Li, Yongqing, Qi, Xinzhu, Wu, Xiushan, Liu, Mingyao. . TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1. In Biochemical and biophysical research communications, 323, 9-16. doi:. https://pubmed.ncbi.nlm.nih.gov/15351693/
8. Shibata, Mio, Sato, Tomonobu, Nukiwa, Ryota, Ariga, Tadashi, Hatakeyama, Shigetsugu. 2012. TRIM45 negatively regulates NF-κB-mediated transcription and suppresses cell proliferation. In Biochemical and biophysical research communications, 423, 104-9. doi:10.1016/j.bbrc.2012.05.090. https://pubmed.ncbi.nlm.nih.gov/22634006/
9. Wang, Jiaxing, Wang, Qiangzhou, Ping, Yuyu, Chang, Guobin, Chen, Shihao. 2024. Identification and characterization of chicken TRIM45 and its role as a negative regulator of ALV-J replication in vitro. In Avian pathology : journal of the W.V.P.A, 54, 255-264. doi:10.1080/03079457.2024.2419039. https://pubmed.ncbi.nlm.nih.gov/39417776/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen