C57BL/6JCya-Prmt2em1/Cya
Common Name:
Prmt2-KO
Product ID:
S-KO-17431
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Prmt2-KO
Strain ID
KOCMP-15468-Prmt2-B6J-VB
Gene Name
Product ID
S-KO-17431
Gene Alias
Hrmt1l1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prmt2em1/Cya mice (Catalog S-KO-17431) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000099572
NCBI RefSeq
NM_001302965
Target Region
Exon 7
Size of Effective Region
~0.2 kb
Detailed Document
Overview of Gene Research
Prmt2, a member of the protein arginine methyltransferase (PRMT) family, acts as a type I enzyme that catalyzes the arginine methylation of target proteins, producing asymmetric dimethyl arginine [2]. It contains a canonical PRMT methylation core and a unique Src homology 3 domain, and is involved in multiple cellular processes such as histone methylation and transcriptional regulation [2].
In cancer research, Prmt2 has been shown to play significant roles. In renal cell cancer (RCC), its overexpression promotes cell proliferation and motility both in vitro and in vivo by enhancing WNT5A transcriptional expression, suggesting it as a novel therapeutic target [1]. In hepatocellular carcinoma (HCC), Prmt2 is overexpressed and is an independent predictor of poor prognosis. Depletion of Prmt2 in HCC cell lines inhibits cell growth and induces apoptosis, as it activates Bcl2 via histone H3R8 methylation [4]. In glioblastomas, Prmt2 is activated by HIF1α under hypoxic conditions, and its inactivation suppresses hypoxia-induced cell migration, attenuates tumor progression, and enhances chemotherapeutic sensitivity [3]. In acute myeloid leukemia (AML), low Prmt2 expressors show inferior survival and increased pro-inflammatory cytokine signaling, indicating Prmt2 as a key regulator of inflammation in AML [5].
Overall, Prmt2 is crucial in various cellular processes and significantly impacts multiple disease conditions, especially in cancer development. Studies using gene knockout models in these diseases have revealed its role in promoting tumorigenesis, metastasis, and regulating inflammation, providing potential therapeutic targets for treatment.
References:
1. Li, Zhongwei, Chen, Chaozhen, Yong, Hongmei, Bai, Jin, Li, Hailong. 2023. PRMT2 promotes RCC tumorigenesis and metastasis via enhancing WNT5A transcriptional expression. In Cell death & disease, 14, 322. doi:10.1038/s41419-023-05837-6. https://pubmed.ncbi.nlm.nih.gov/37173306/
2. Cura, Vincent, Cavarelli, Jean. 2021. Structure, Activity and Function of the PRMT2 Protein Arginine Methyltransferase. In Life (Basel, Switzerland), 11, . doi:10.3390/life11111263. https://pubmed.ncbi.nlm.nih.gov/34833139/
3. Dong, Feng, Sun, Xiaoyu, Su, Jiacheng, Xu, Guogang, Wu, Xudong. 2024. Hypoxia-inducible PRMT2 addiction in glioblastomas. In Cellular signalling, 117, 111094. doi:10.1016/j.cellsig.2024.111094. https://pubmed.ncbi.nlm.nih.gov/38341123/
4. Hu, Guohui, Yan, Chen, Xie, Peiyi, Shao, Jia, Ge, Jin. 2020. PRMT2 accelerates tumorigenesis of hepatocellular carcinoma by activating Bcl2 via histone H3R8 methylation. In Experimental cell research, 394, 112152. doi:10.1016/j.yexcr.2020.112152. https://pubmed.ncbi.nlm.nih.gov/32574605/
5. Sauter, Camille, Morin, Thomas, Guidez, Fabien, Aucagne, Romain, Delva, Laurent. 2024. Protein arginine methyltransferase 2 controls inflammatory signaling in acute myeloid leukemia. In Communications biology, 7, 753. doi:10.1038/s42003-024-06453-6. https://pubmed.ncbi.nlm.nih.gov/38902349/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen