C57BL/6JCya-Magi1em1/Cya
Common Name:
Magi1-KO
Product ID:
S-KO-17463
Background:
C57BL/6JCya
Product Type
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Genotype
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Basic Information
Strain Name
Magi1-KO
Strain ID
KOCMP-14924-Magi1-B6J-VB
Gene Name
Product ID
S-KO-17463
Gene Alias
AIP3; BAP1; Baiap1; Gukmi1; MAGI1c; Magi-1; TNRC19; WWP3; mKIAA4129
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Magi1em1/Cya mice (Catalog S-KO-17463) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000089317
NCBI RefSeq
NM_001029850.4
Target Region
Exon 5
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
MAGI1, short for membrane-associated guanylate kinase with inverted domain structure-1, is a cytoplasmic scaffolding protein. It stabilizes cell-to-cell contacts, regulating cadherin-mediated cell-cell adhesion in epithelial and endothelial cells. It is involved in crucial pathways like PI3K/AKT and Wnt/β-catenin, and is of great importance in tumor suppression and vascular functions. Genetic models, such as KO mouse models, can be valuable in studying its functions [1].
In various cancers, MAGI1 shows tumor-suppressive properties. In glioma, its low expression is related to malignant progression, and overexpression inhibits cell proliferation, migration, and invasion through regulating pathways like Akt, MMP2, MMP9, and E-cadherin/N-cadherin/vimentin [3]. In ER + breast cancer, MAGI1 loss leads to deeper quiescence/senescence, reduced DNA repair protein expression, and increased sensitivity to PARP1 inhibition. Also, in luminal breast cancer, MAGI1 acts as a tumor-suppressor by inhibiting the AMOTL2/p38 stress pathway [5,6]. In influenza A virus (IAV) infection, MAGI1 promotes infection by inhibiting interferon signaling. Depletion of MAGI1 suppresses IAV infection, indicating its potential as a therapeutic target for IAV-induced cardiovascular disease [2]. In endothelial cells, MAGI1 localizes at mature focal adhesion, regulating integrin-mediated adhesion and signaling. It also plays a role in endothelial cell activation and atherogenesis, with its phosphorylation and deSUMOylation regulating EC activation and apoptosis [4,7].
In conclusion, MAGI1 is a key protein with tumor-suppressive functions in multiple cancers and a role in viral infection and vascular-related diseases. Studies using gene-knockout models have revealed its importance in regulating cell proliferation, apoptosis, adhesion, and signaling pathways in these disease contexts, providing insights into potential therapeutic strategies.
References:
1. Wörthmüller, Janine, Rüegg, Curzio. 2021. MAGI1, a Scaffold Protein with Tumor Suppressive and Vascular Functions. In Cells, 10, . doi:10.3390/cells10061494. https://pubmed.ncbi.nlm.nih.gov/34198584/
2. Wang, Yin, Abe, Jun-Ichi, Chau, Khanh M, Evans, Scott E, Le, Nhat-Tu. 2022. MAGI1 inhibits interferon signaling to promote influenza A infection. In Frontiers in cardiovascular medicine, 9, 791143. doi:10.3389/fcvm.2022.791143. https://pubmed.ncbi.nlm.nih.gov/36082118/
3. Li, Zhong-Yan, Li, Xue-Hua, Tian, Guang-Wei, Gao, Hai, Wang, Zhen-Yu. 2019. MAGI1 Inhibits the Proliferation, Migration and Invasion of Glioma Cells. In OncoTargets and therapy, 12, 11281-11290. doi:10.2147/OTT.S230236. https://pubmed.ncbi.nlm.nih.gov/31908493/
4. Abe, Jun-Ichi, Ko, Kyung Ae, Kotla, Sivareddy, Evans, Scott E, Le, Nhat-Tu. 2019. MAGI1 as a link between endothelial activation and ER stress drives atherosclerosis. In JCI insight, 4, . doi:10.1172/jci.insight.125570. https://pubmed.ncbi.nlm.nih.gov/30944250/
5. Wörthmüller, Janine, Disler, Simona, Pradervand, Sylvain, Desmedt, Christine, Rüegg, Curzio. 2023. MAGI1 Prevents Senescence and Promotes the DNA Damage Response in ER+ Breast Cancer. In Cells, 12, . doi:10.3390/cells12151929. https://pubmed.ncbi.nlm.nih.gov/37566008/
6. Kantar, Diala, Mur, Emilie Bousquet, Mancini, Maicol, Heron-Milhavet, Lisa, Djiane, Alexandre. 2021. MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis. In Scientific reports, 11, 5752. doi:10.1038/s41598-021-85056-1. https://pubmed.ncbi.nlm.nih.gov/33707576/
7. Alday-Parejo, Begoña, Ghimire, Kedar, Coquoz, Oriana, Stalin, Jimmy, Rüegg, Curzio. . MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis. In Cell adhesion & migration, 15, 126-139. doi:10.1080/19336918.2021.1911472. https://pubmed.ncbi.nlm.nih.gov/33823745/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen