C57BL/6JCya-Ppp1r9bem1/Cya
Common Name:
Ppp1r9b-KO
Product ID:
S-KO-17514
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ppp1r9b-KO
Strain ID
KOCMP-217124-Ppp1r9b-B6J-VC
Gene Name
Product ID
S-KO-17514
Gene Alias
SPL; Spn
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp1r9bem1/Cya mice (Catalog S-KO-17514) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038696
NCBI RefSeq
NM_172261
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Ppp1r9b, also known as SPINOPHILIN or NEURABIN-2, is a multifunctional protein and one of the regulatory subunits of protein phosphatase 1 (PP1). It is involved in regulating protein - protein interactions in different cell signaling pathways, and plays a role in cell cycle regulation by participating in the dephosphorylation of retinoblastoma protein (pRB) [1,2,3,4].
Mutations in PPP1R9B, such as the SPN - A566V mutation, can affect its interaction with PP1 and phosphatase activity, promoting the tumorigenic and stemness properties of cells, especially in cells with mutant p53 [1]. Down - regulation of Ppp1r9b in breast and lung tumors increases the cancer stem cell population, correlates with a worse prognosis, and contributes to tumorigenesis [3,4].
In conclusion, Ppp1r9b is crucial for normal cell function, especially in cell cycle regulation. Its dysregulation, either through mutation or down - regulation, has significant implications in tumorigenesis, highlighting its importance as a potential target for cancer research. The study of Ppp1r9b in KO/CKO mouse models, as well as in human tumor samples, provides valuable insights into its role in cancer development and progression [1,3,4].
References:
1. Verdugo-Sivianes, Eva M, Rojas, Ana M, Muñoz-Galván, Sandra, Otero-Albiol, Daniel, Carnero, Amancio. 2021. Mutation of SPINOPHILIN (PPP1R9B) found in human tumors promotes the tumorigenic and stemness properties of cells. In Theranostics, 11, 3452-3471. doi:10.7150/thno.53572. https://pubmed.ncbi.nlm.nih.gov/33537097/
2. Verdugo-Sivianes, Eva M, Carnero, Amancio. 2022. SPINOPHILIN: A multiplayer tumor suppressor. In Genes & diseases, 10, 187-198. doi:10.1016/j.gendis.2021.12.021. https://pubmed.ncbi.nlm.nih.gov/37013033/
3. Ferrer, I, Verdugo-Sivianes, E M, Castilla, M A, Palacios, J, Carnero, A. 2015. Loss of the tumor suppressor spinophilin (PPP1R9B) increases the cancer stem cell population in breast tumors. In Oncogene, 35, 2777-88. doi:10.1038/onc.2015.341. https://pubmed.ncbi.nlm.nih.gov/26387546/
4. Molina-Pinelo, Sonia, Ferrer, Irene, Blanco-Aparicio, Carmen, Paz-Ares, Luis, Carnero, Amancio. 2011. Down-regulation of spinophilin in lung tumours contributes to tumourigenesis. In The Journal of pathology, 225, 73-82. doi:10.1002/path.2905. https://pubmed.ncbi.nlm.nih.gov/21598252/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen