C57BL/6JCya-Kri1em1/Cya
Common Name:
Kri1-KO
Product ID:
S-KO-17568
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Kri1-KO
Strain ID
KOCMP-215194-Kri1-B6J-VB
Gene Name
Product ID
S-KO-17568
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kri1em1/Cya mice (Catalog S-KO-17568) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038671
NCBI RefSeq
NM_145416
Target Region
Exon 5~11
Size of Effective Region
~3.0 kb
Overview of Gene Research
Kri1, also known as KRR1 interacting protein, is essential for 40S ribosome biogenesis in the nucleolus. It physically and functionally interacts with Krr1p to form a complex crucial for this process [2]. Kri1 is also involved in other biological processes and diseases. It has been associated with iron deficiency anemia, as rare mutations in Kri1 in a patient may potentially cause intestinal iron loss [3]. In esophageal carcinoma, Kri1 was identified as one of the ferroptosis-related key genes, with the highest mutation frequency among certain genes, and its associated prognostic complex value could predict patient survival and tumor-infiltrating immune cells [1].
In C. elegans, Kri1 (KRI-1 ortholog of mammalian KRIT1/CCM1) exists in a complex with CCM-2 in the intestine. This complex negatively regulates the ERK-5/MAPK pathway, allowing the expression of the SLC39 zinc transporter gene zipt-2.3 to sequester zinc in the intestine. Ablation of KRI-1 leads to reduced zinc sequestration, inhibition of IR-induced MPK-1/ERK1 activation, and apoptosis in the germline [4].
In conclusion, Kri1 plays essential roles in ribosome biogenesis, and its functions in iron metabolism-related anemia and apoptosis regulation through zinc homeostasis are significant. Studies in model organisms like C. elegans have provided insights into its role in disease-related processes, such as the potential link between zinc localization and cerebral cavernous malformation disease [4].
References:
1. Wang, Jian, Guo, Ziming, Sun, Fei, Wang, Jianlin, Yu, Jingping. 2022. A Novel Ferroptosis-Related Gene Signature to Predict Prognosis of Esophageal Carcinoma. In Journal of oncology, 2022, 7485435. doi:10.1155/2022/7485435. https://pubmed.ncbi.nlm.nih.gov/35813863/
2. Sasaki, T, Toh-E, A, Kikuchi, Y. . Yeast Krr1p physically and functionally interacts with a novel essential Kri1p, and both proteins are required for 40S ribosome biogenesis in the nucleolus. In Molecular and cellular biology, 20, 7971-9. doi:. https://pubmed.ncbi.nlm.nih.gov/11027267/
3. Huettmann, Carolina, Stelljes, Matthias, Sivalingam, Sugirthan, Muckenthaler, Martina U, Steinbicker, Andrea U. 2021. Iron Deficiency Caused by Intestinal Iron Loss-Novel Candidate Genes for Severe Anemia. In Genes, 12, . doi:10.3390/genes12121869. https://pubmed.ncbi.nlm.nih.gov/34946818/
4. Chapman, Eric M, Lant, Benjamin, Ohashi, Yota, Gingras, Anne-Claude, Derry, W Brent. 2019. A conserved CCM complex promotes apoptosis non-autonomously by regulating zinc homeostasis. In Nature communications, 10, 1791. doi:10.1038/s41467-019-09829-z. https://pubmed.ncbi.nlm.nih.gov/30996251/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen