C57BL/6JCya-Kri1em1/Cya
Common Name:
Kri1-KO
Product ID:
S-KO-17568
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Kri1-KO
Strain ID
KOCMP-215194-Kri1-B6J-VB
Gene Name
Product ID
S-KO-17568
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kri1em1/Cya mice (Catalog S-KO-17568) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038671
NCBI RefSeq
NM_145416
Target Region
Exon 5~11
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Kri1, also known as KRR1 interacting protein, is essential for 40S ribosome biogenesis in the nucleolus. It physically and functionally interacts with Krr1p to form a complex crucial for this process [2]. Kri1 is also involved in other biological processes and diseases. It has been associated with iron deficiency anemia, as rare mutations in Kri1 in a patient may potentially cause intestinal iron loss [3]. In esophageal carcinoma, Kri1 was identified as one of the ferroptosis-related key genes, with the highest mutation frequency among certain genes, and its associated prognostic complex value could predict patient survival and tumor-infiltrating immune cells [1].
In C. elegans, Kri1 (KRI-1 ortholog of mammalian KRIT1/CCM1) exists in a complex with CCM-2 in the intestine. This complex negatively regulates the ERK-5/MAPK pathway, allowing the expression of the SLC39 zinc transporter gene zipt-2.3 to sequester zinc in the intestine. Ablation of KRI-1 leads to reduced zinc sequestration, inhibition of IR-induced MPK-1/ERK1 activation, and apoptosis in the germline [4].
In conclusion, Kri1 plays essential roles in ribosome biogenesis, and its functions in iron metabolism-related anemia and apoptosis regulation through zinc homeostasis are significant. Studies in model organisms like C. elegans have provided insights into its role in disease-related processes, such as the potential link between zinc localization and cerebral cavernous malformation disease [4].
References:
1. Wang, Jian, Guo, Ziming, Sun, Fei, Wang, Jianlin, Yu, Jingping. 2022. A Novel Ferroptosis-Related Gene Signature to Predict Prognosis of Esophageal Carcinoma. In Journal of oncology, 2022, 7485435. doi:10.1155/2022/7485435. https://pubmed.ncbi.nlm.nih.gov/35813863/
2. Sasaki, T, Toh-E, A, Kikuchi, Y. . Yeast Krr1p physically and functionally interacts with a novel essential Kri1p, and both proteins are required for 40S ribosome biogenesis in the nucleolus. In Molecular and cellular biology, 20, 7971-9. doi:. https://pubmed.ncbi.nlm.nih.gov/11027267/
3. Huettmann, Carolina, Stelljes, Matthias, Sivalingam, Sugirthan, Muckenthaler, Martina U, Steinbicker, Andrea U. 2021. Iron Deficiency Caused by Intestinal Iron Loss-Novel Candidate Genes for Severe Anemia. In Genes, 12, . doi:10.3390/genes12121869. https://pubmed.ncbi.nlm.nih.gov/34946818/
4. Chapman, Eric M, Lant, Benjamin, Ohashi, Yota, Gingras, Anne-Claude, Derry, W Brent. 2019. A conserved CCM complex promotes apoptosis non-autonomously by regulating zinc homeostasis. In Nature communications, 10, 1791. doi:10.1038/s41467-019-09829-z. https://pubmed.ncbi.nlm.nih.gov/30996251/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen