C57BL/6JCya-Nsun3em1/Cya
Common Name
Nsun3-KO
Product ID
S-KO-17797
Backgroud
C57BL/6JCya
Strain ID
KOCMP-106338-Nsun3-B6J-VB
When using this mouse strain in a publication, please cite “Nsun3-KO Mouse (Catalog S-KO-17797) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nsun3-KO
Strain ID
KOCMP-106338-Nsun3-B6J-VB
Gene Name
Product ID
S-KO-17797
Gene Alias
6720484A09Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 16
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000063089
NCBI RefSeq
NM_178925
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Overview of Gene Research
NSUN3, also known as NOP2/Sun RNA methyltransferase 3, is a mitochondrial methyltransferase. It is crucial for initiating the 5-formylcytidine (f5C) modification in mitochondrial methionine tRNA anticodon, which is essential for the translation of the AUA codon in mammalian mitochondria. This modification is involved in mitochondrial function-related pathways, and is of great biological importance for embryonic development and normal physiological function of adult tissues [1,2,3].
Whole-body Nsun3 knockout in mice leads to embryonic death between E10.5 and E12.5, highlighting its essential role in embryonic development [1]. Heart-specific Nsun3 knockout (Nsun3HKO) mice show enlarged heart mitochondria with fragmented cristae, enhanced heart contraction, and age-associated mild heart enlargement. In Nsun3HKO hearts, the enzymatic activities of respiratory complexes decrease, despite no down-regulation of mitochondrial mRNAs encoding respiratory complex subunits [1].
In conclusion, NSUN3 is essential for mammalian embryonic development and normal mitochondrial function in adult tissues. Studies using Nsun3 KO and CKO mouse models have revealed its significance in embryonic development and heart-related pathologies, providing valuable insights into understanding the role of mitochondrial tRNA modification in these biological processes and associated diseases [1].
References：
1. Murakami, Yoshitaka, Wei, Fan-Yan, Kawamura, Yoshimi, Tomizawa, Kazuhito, Chujo, Takeshi. 2023. NSUN3-mediated mitochondrial tRNA 5-formylcytidine modification is essential for embryonic development and respiratory complexes in mice. In Communications biology, 6, 307. doi:10.1038/s42003-023-04680-x. https://pubmed.ncbi.nlm.nih.gov/36949224/
2. Nakano, Saori, Suzuki, Takeo, Kawarada, Layla, Asano, Kana, Suzuki, Tsutomu. 2016. NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met). In Nature chemical biology, 12, 546-51. doi:10.1038/nchembio.2099. https://pubmed.ncbi.nlm.nih.gov/27214402/
3. Haag, Sara, Sloan, Katherine E, Ranjan, Namit, Höbartner, Claudia, Bohnsack, Markus T. 2016. NSUN3 and ABH1 modify the wobble position of mt-tRNAMet to expand codon recognition in mitochondrial translation. In The EMBO journal, 35, 2104-2119. doi:. https://pubmed.ncbi.nlm.nih.gov/27497299/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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