C57BL/6JCya-Edem1em1/Cya
Common Name:
Edem1-KO
Product ID:
S-KO-17812
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Edem1-KO
Strain ID
KOCMP-192193-Edem1-B6J-VB
Gene Name
Product ID
S-KO-17812
Gene Alias
A130059K23Rik; EDEM; mKIAA0212
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Edem1em1/Cya mice (Catalog S-KO-17812) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000089162
NCBI RefSeq
NM_138677
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Edem1, also known as endoplasmic reticulum degradation-enhancing α-mannosidase-like protein 1, is a key quality control factor in the endoplasmic reticulum (ER) [1,2,3,4,5,6,7]. It is directly involved in the endoplasmic reticulum-associated degradation (ERAD) process, recognizing terminally misfolded proteins and guiding them to retrotranslocation for proteasomal degradation in the cytosol, thus maintaining ER homeostasis [1,3,6].
In terms of specific functions, in human cells, EDEM1 is an important regulator of amyloid precursor protein (APP) metabolism. Overproduction of EDEM1 reduces APP cellular levels and Aβ40 and Aβ42 secretion, while down-regulation of EDEM1 increases APP levels, suggesting its role in Alzheimer's disease-related processes [1]. In pancreatic β-cells, EDEM1 overexpression inhibits the IRE1/JNK/c-Jun pathway, increasing insulin mRNA levels and insulin secretion, which is relevant to diabetes treatment [2]. In Drosophila, Edem1 in the fat body regulates insulin signalling and metabolic homeostasis by limiting the activity of Drosophila tumor necrosis factor-α Eiger on insulin-producing cells [5]. Also, EDEM1 itself is turned over by ERAD and autophagy, and its degradation is mediated by multiple routes and various ERAD factors [3]. Alterations in EDEM1 functions can enhance ATF6 pro-survival signalling, and EDEM1 can drive misfolded protein degradation via ERAD and use ER-phagy as a backup mechanism when ERAD is impaired [4,6].
In conclusion, Edem1 is crucial for maintaining protein quality control in the ER and has far-reaching impacts on multiple biological processes and disease-related pathways. Its functions in APP metabolism, insulin regulation, and maintaining metabolic homeostasis, as revealed through various studies, highlight its significance in diseases such as Alzheimer's and diabetes. Understanding Edem1 through different research models helps in uncovering the underlying molecular mechanisms of these diseases and potentially developing new treatment strategies.
References:
1. Nowakowska-Gołacka, Jowita, Czapiewska, Justyna, Sominka, Hanna, Sowa-Rogozińska, Natalia, Słomińska-Wojewódzka, Monika. 2021. EDEM1 Regulates Amyloid Precursor Protein (APP) Metabolism and Amyloid-β Production. In International journal of molecular sciences, 23, . doi:10.3390/ijms23010117. https://pubmed.ncbi.nlm.nih.gov/35008544/
2. Flintoaca Alexandru, Petruta R, Chiritoiu, Gabriela N, Lixandru, Daniela, Ionescu-Targoviste, Constantin, Petrescu, Stefana M. 2023. EDEM1 regulates the insulin mRNA level by inhibiting the endoplasmic reticulum stress-induced IRE1/JNK/c-Jun pathway. In iScience, 26, 107956. doi:10.1016/j.isci.2023.107956. https://pubmed.ncbi.nlm.nih.gov/37822496/
3. Katsuki, Riko, Kanuka, Mai, Ohta, Ren, Yoshida, Shusei, Tamura, Taku. 2024. Turnover of EDEM1, an ERAD-enhancing factor, is mediated by multiple degradation routes. In Genes to cells : devoted to molecular & cellular mechanisms, 29, 486-502. doi:10.1111/gtc.13117. https://pubmed.ncbi.nlm.nih.gov/38682256/
4. Papaioannou, Alexandra, Higa, Arisa, Jégou, Gwénaële, Pluquet, Olivier, Chevet, Eric. 2018. Alterations of EDEM1 functions enhance ATF6 pro-survival signaling. In The FEBS journal, 285, 4146-4164. doi:10.1111/febs.14669. https://pubmed.ncbi.nlm.nih.gov/30281916/
5. Pathak, Himani, Varghese, Jishy. 2021. Edem1 activity in the fat body regulates insulin signalling and metabolic homeostasis in Drosophila. In Life science alliance, 4, . doi:10.26508/lsa.202101079. https://pubmed.ncbi.nlm.nih.gov/34140347/
6. Chiritoiu, Marioara, Chiritoiu, Gabriela N, Munteanu, Cristian V A, Ivessa, N Erwin, Petrescu, Stefana M. 2020. EDEM1 Drives Misfolded Protein Degradation via ERAD and Exploits ER-Phagy as Back-Up Mechanism When ERAD Is Impaired. In International journal of molecular sciences, 21, . doi:10.3390/ijms21103468. https://pubmed.ncbi.nlm.nih.gov/32423001/
7. Lamriben, Lydia, Oster, Michela E, Tamura, Taku, Clausen, Henrik, Hebert, Daniel N. 2018. EDEM1's mannosidase-like domain binds ERAD client proteins in a redox-sensitive manner and possesses catalytic activity. In The Journal of biological chemistry, 293, 13932-13945. doi:10.1074/jbc.RA118.004183. https://pubmed.ncbi.nlm.nih.gov/30021839/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen