C57BL/6JCya-Rgccem1/Cya
Common Name:
Rgcc-KO
Product ID:
S-KO-17883
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rgcc-KO
Strain ID
KOCMP-66214-Rgcc-B6J-VA
Gene Name
Product ID
S-KO-17883
Gene Alias
1190002H23Rik; Rgc-32; Rgc32
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rgccem1/Cya mice (Catalog S-KO-17883) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022595
NCBI RefSeq
NM_025427
Target Region
Exon 1~2
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
RGCC, also known as regulator of cell cycle or response gene to complement 32 protein, is involved in multiple biological processes. It plays a role in cell cycle regulation, and is associated with pathways related to metabolism, fibrosis, and neural development. Genetic models, such as KO mouse models, are valuable for studying its functions, as they can help reveal its role in specific biological processes and disease conditions [1-4].
In triple-negative breast cancer (TNBC), RGCC is aberrantly upregulated in pulmonary metastatic cells. It promotes the kinase activity of PLK1, which phosphorylates AMPKα2, facilitating TNBC lung metastasis by increasing oxidative phosphorylation and fatty acid oxidation [1].
In pulmonary fibrosis, RGCC levels decrease, and overexpression of RGCC in lung fibroblasts attenuates the effects of TGF-β on collagen levels. Mice with constitutive RGCC gene deletion accumulate more collagen in their lungs in response to bleomycin [2].
In neocortical development, RGCC deficiency hampers normal cell cycle process and dysregulates the mitotic spindle, leading to abnormal NSC differentiation and brain malformation [3].
In medaka spermatogonia, Smo mediates spermatogonia proliferation through Gli1-Rgcc-Cdk1 axis, and Rgcc overexpression rescues cell apoptosis caused by Smo or Gli1 inhibition [4].
In conclusion, RGCC is essential for maintaining normal cell cycle, metabolism, and tissue homeostasis. KO/CKO mouse models have contributed to understanding its role in diseases like TNBC lung metastasis and pulmonary fibrosis. These findings suggest that targeting RGCC could potentially be a therapeutic strategy for these diseases.
References:
1. Cheng, Shaojie, Wan, Xueying, Yang, Liping, Wu, Mingjun, Liu, Manran. 2023. RGCC-mediated PLK1 activity drives breast cancer lung metastasis by phosphorylating AMPKα2 to activate oxidative phosphorylation and fatty acid oxidation. In Journal of experimental & clinical cancer research : CR, 42, 342. doi:10.1186/s13046-023-02928-2. https://pubmed.ncbi.nlm.nih.gov/38102722/
2. Luzina, Irina G, Rus, Violeta, Lockatell, Virginia, Rus, Horea, Atamas, Sergei P. . Regulator of Cell Cycle Protein (RGCC/RGC-32) Protects against Pulmonary Fibrosis. In American journal of respiratory cell and molecular biology, 66, 146-157. doi:10.1165/rcmb.2021-0022OC. https://pubmed.ncbi.nlm.nih.gov/34668840/
3. Guo, Zhenming, Chen, Mengxia, Chao, Yiming, Bian, Shan, He, Guang. 2021. RGCC balances self-renewal and neuronal differentiation of neural stem cells in the developing mammalian neocortex. In EMBO reports, 22, e51781. doi:10.15252/embr.202051781. https://pubmed.ncbi.nlm.nih.gov/34323349/
4. Zhao, Changle, Liu, Xiang, Liu, Lei, Wang, Deshou, Wei, Jing. . Smoothened mediates medaka spermatogonia proliferation via Gli1-Rgcc-Cdk1 axis†. In Biology of reproduction, 109, 772-784. doi:10.1093/biolre/ioad090. https://pubmed.ncbi.nlm.nih.gov/37552059/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen