C57BL/6JCya-Dpysl2em1/Cya
Common Name:
Dpysl2-KO
Product ID:
S-KO-18024
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dpysl2-KO
Strain ID
KOCMP-12934-Dpysl2-B6J-VB
Gene Name
Product ID
S-KO-18024
Gene Alias
Crmp2; DRP2; Musunc33; TOAD-64; Ulip2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dpysl2em1/Cya mice (Catalog S-KO-18024) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022629
NCBI RefSeq
NM_009955.3
Target Region
Exon 3
Size of Effective Region
~185 bp
Detailed Document
Overview of Gene Research
DPYSL2, also known as CRMP2, encodes a microtubule-stabilizing protein of great significance in neurogenesis. It is associated with multiple pathways such as mTOR signaling, cytoskeletal dynamics, immune function, calcium signaling, and cholesterol biosynthesis [1,3,5]. This gene is linked to numerous psychiatric and neurodegenerative disorders, including schizophrenia, bipolar disorder, and Alzheimer's disease, highlighting its overall biological importance [1]. Genetic models, like knockout models, have been crucial in understanding its functions.
In human iPSC-derived glutamatergic neurons, knockout of DPYSL2-B (an isoform of DPYSL2) led to a reduction in dendrite length and disruptions in pathways relevant to psychiatric diseases, confirming its role in mTOR signaling and neurodevelopmental disorders [1]. In zebrafish, dpysl2 knockout caused abnormal migration of facial branchiomotor neurons during early development [4]. In breast cancer cells, DPYSL2 knockout profoundly inhibited cell migration, invasion, stemness features, tumor growth rate, and metastasis [2].
In conclusion, DPYSL2 is essential for neurogenesis, neuronal migration, and has an impact on cancer cell behavior. Gene knockout models, especially in neurons and zebrafish, have been instrumental in revealing its role in neurodevelopmental disorders and cancer-related processes, providing valuable insights into the underlying molecular mechanisms of these diseases.
References:
1. Feuer, Kyra L, Peng, Xi, Yovo, Christian K, Avramopoulos, Dimitrios. 2023. DPYSL2/CRMP2 isoform B knockout in human iPSC-derived glutamatergic neurons confirms its role in mTOR signaling and neurodevelopmental disorders. In Molecular psychiatry, 28, 4353-4362. doi:10.1038/s41380-023-02186-w. https://pubmed.ncbi.nlm.nih.gov/37479784/
2. Abu Rmaileh, Areej, Solaimuthu, Balakrishnan, Khatib, Anees, Pillar, Nir, Shaul, Yoav D. 2022. DPYSL2 interacts with JAK1 to mediate breast cancer cell migration. In The Journal of cell biology, 221, . doi:10.1083/jcb.202106078. https://pubmed.ncbi.nlm.nih.gov/35575798/
3. Izumi, Ryuta, Hino, Mizuki, Nagaoka, Atsuko, Kunii, Yasuto, Yabe, Hirooki. 2021. Dysregulation of DPYSL2 expression by mTOR signaling in schizophrenia: Multi-level study of postmortem brain. In Neuroscience research, 175, 73-81. doi:10.1016/j.neures.2021.09.004. https://pubmed.ncbi.nlm.nih.gov/34543692/
4. Fiallos-Oliveros, Carolina, Ohshima, Toshio. . Dpysl2 (CRMP2) is required for the migration of facial branchiomotor neurons in the developing zebrafish embryo. In The International journal of developmental biology, 64, 479-484. doi:10.1387/ijdb.190375to. https://pubmed.ncbi.nlm.nih.gov/33336710/
5. Pham, X, Song, G, Lao, S, Valle, D, Avramopoulos, D. 2016. The DPYSL2 gene connects mTOR and schizophrenia. In Translational psychiatry, 6, e933. doi:10.1038/tp.2016.204. https://pubmed.ncbi.nlm.nih.gov/27801893/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen