C57BL/6JCya-Dnmt3lem1/Cya
Common Name
Dnmt3l-KO
Product ID
S-KO-18049
Backgroud
C57BL/6JCya
Strain ID
KOCMP-54427-Dnmt3l-B6J-VB
When using this mouse strain in a publication, please cite “Dnmt3l-KO Mouse (Catalog S-KO-18049) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Dnmt3l-KO
Strain ID
KOCMP-54427-Dnmt3l-B6J-VB
Gene Name
Product ID
S-KO-18049
Gene Alias
D6Ertd14e, ecat7
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000138785
NCBI RefSeq
NM_001081695
Target Region
Exon 6~10
Size of Effective Region
~4.7 kb
Overview of Gene Research
Dnmt3l, a member of the DNMT3 family, has no DNA methyltransferase activity but is crucial for regulating de novo DNA methylation. It integrates DNA methylation and histone modification-based epigenetic circuits, and is involved in various biological processes such as neural development, stem cell regulation, and germ cell DNA methylation [2,3,5]. Genetic models like KO or CKO mouse models are valuable for studying its functions.
In Dnmt3l-deficient mouse embryonic stem cells (mESCs), there is down-regulation of DNMT3A, especially DNMT3A2, leading to hypomethylation at many DNMT3A target regions, indicating Dnmt3l is a positive regulator of DNA methylation as it maintains DNMT3A stability [2].
In Dnmt3l conditional knockin mice, overexpression of Dnmt3l promotes neural differentiation by enhancing STAT1 and STAT3 phosphorylation independent of DNA methylation [1].
In postnatal testes, Dnmt3l-KO in THY1(+) spermatogonial stem/progenitor cells (SPCs) leads to reduced promyelocytic leukemia zinc finger (PLZF) stability, over-activation of ERK and AKT signaling cascades, and premature stem cell exhaustion, suggesting Dnmt3l is required to balance SPC cycling and quiescence [4].
Also, using Dnmt3l-knockout donor cells in combination with Trichostatin A treatment improves the developmental efficiency and quality of cloned embryos, with Dnmt3l-KO donor cells offering a more permissive epigenetic state for nuclear transfer reprogramming [6].
In conclusion, Dnmt3l plays essential roles in DNA methylation, neural differentiation, and stem cell fate determination. Studies using KO/CKO mouse models have revealed its significance in diseases like Down syndrome-related abnormal neurodevelopment, highlighting its potential as a therapeutic target for understanding and treating such conditions [1,7].
References：
1. Qin, Lin, Qiao, Chong, Sheen, Volney, Wang, Yu, Lu, Jie. 2021. DNMT3L promotes neural differentiation by enhancing STAT1 and STAT3 phosphorylation independent of DNA methylation. In Progress in neurobiology, 201, 102028. doi:10.1016/j.pneurobio.2021.102028. https://pubmed.ncbi.nlm.nih.gov/33636226/
2. Veland, Nicolas, Lu, Yue, Hardikar, Swanand, Zhao, Hongbo, Chen, Taiping. . DNMT3L facilitates DNA methylation partly by maintaining DNMT3A stability in mouse embryonic stem cells. In Nucleic acids research, 47, 152-167. doi:10.1093/nar/gky947. https://pubmed.ncbi.nlm.nih.gov/30321403/
3. Kubo, Naoki, Uehara, Ryuji, Uemura, Shuhei, Shirane, Kenjiro, Sasaki, Hiroyuki. 2024. Combined and differential roles of ADD domains of DNMT3A and DNMT3L on DNA methylation landscapes in mouse germ cells. In Nature communications, 15, 3266. doi:10.1038/s41467-024-47699-2. https://pubmed.ncbi.nlm.nih.gov/38627502/
4. Liao, Hung-Fu, Chen, Wendy S C, Chen, Yu-Hsiang, Yen, Pauline, Lin, Shau-Ping. 2014. DNMT3L promotes quiescence in postnatal spermatogonial progenitor cells. In Development (Cambridge, England), 141, 2402-13. doi:10.1242/dev.105130. https://pubmed.ncbi.nlm.nih.gov/24850856/
5. Basu, Amitava, Tomar, Archana, Dasari, Vasanthi, Mishra, Rakesh Kumar, Khosla, Sanjeev. 2016. DNMT3L enables accumulation and inheritance of epimutations in transgenic Drosophila. In Scientific reports, 6, 19572. doi:10.1038/srep19572. https://pubmed.ncbi.nlm.nih.gov/26795243/
6. Liao, Hung-Fu, Mo, Chu-Fan, Wu, Shinn-Chih, Sung, Li-Ying, Lin, Shau-Ping. 2015. Dnmt3l-knockout donor cells improve somatic cell nuclear transfer reprogramming efficiency. In Reproduction (Cambridge, England), 150, 245-56. doi:10.1530/REP-15-0031. https://pubmed.ncbi.nlm.nih.gov/26159833/
7. Laufer, Benjamin I, Gomez, J Antonio, Jianu, Julia M, LaSalle, Janine M. 2021. Stable DNMT3L overexpression in SH-SY5Y neurons recreates a facet of the genome-wide Down syndrome DNA methylation signature. In Epigenetics & chromatin, 14, 13. doi:10.1186/s13072-021-00387-7. https://pubmed.ncbi.nlm.nih.gov/33750431/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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