C57BL/6JCya-Gtf2h4em1/Cya
Common Name:
Gtf2h4-KO
Product ID:
S-KO-18129
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gtf2h4-KO
Strain ID
KOCMP-14885-Gtf2h4-B6J-VB
Gene Name
Product ID
S-KO-18129
Gene Alias
BTF2 p52; p52
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gtf2h4em1/Cya mice (Catalog S-KO-18129) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001565
NCBI RefSeq
NM_010364
Target Region
Exon 4~11
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Gtf2h4, also known as general transcription factor IIH subunit 4, is involved in transcription-dependent DNA repair mechanisms [2]. It may play a role in regulating gene expression, which is crucial for maintaining genomic integrity and normal cellular functions.
In ischemic diseases, Gtf2h4 has been shown to regulate partial endothelial-to-mesenchymal transition (EndMT) via NF-κB activation through NCOA3 phosphorylation. In vivo experiments confirmed that Gtf2h4 significantly promoted partial EndMT and angiogenesis after ischemic injury [1].
In prostate cancer, high expression of Gtf2h4 is associated with a higher biochemical recurrence rate, indicating it may be a risk factor for poor prognosis [4]. Also, a genetic variant in Gtf2h4 has been associated with lung cancer risk, where the variant allele was significantly associated with decreased DNA repair capacity phenotype and decreased mRNA expression levels [3].
In summary, Gtf2h4 is important in DNA repair-related functions and has significant implications in diseases such as ischemic diseases, prostate cancer, and lung cancer. The findings from in vivo studies in ischemic injury models and genetic association studies in cancer patients highlight its role in disease-related biological processes, providing potential therapeutic targets for these diseases.
References:
1. Fang, Zheyan, Zhao, Gang, Zhao, Shuang, Ge, Junbo, Li, Hua. 2024. GTF2H4 regulates partial EndMT via NF-κB activation through NCOA3 phosphorylation in ischemic diseases. In Innovation (Cambridge (Mass.)), 5, 100565. doi:10.1016/j.xinn.2024.100565. https://pubmed.ncbi.nlm.nih.gov/38379791/
2. Porter, Louise F, Saptarshi, Neil, Fang, Yongxiang, Chavali, Venkata R M, Paraoan, Luminita. 2019. Whole-genome methylation profiling of the retinal pigment epithelium of individuals with age-related macular degeneration reveals differential methylation of the SKI, GTF2H4, and TNXB genes. In Clinical epigenetics, 11, 6. doi:10.1186/s13148-019-0608-2. https://pubmed.ncbi.nlm.nih.gov/30642396/
3. Wang, Meilin, Liu, Hongliang, Liu, Zhensheng, Amos, Christopher I, Wei, Qingyi. 2016. Genetic variant in DNA repair gene GTF2H4 is associated with lung cancer risk: a large-scale analysis of six published GWAS datasets in the TRICL consortium. In Carcinogenesis, 37, 888-896. doi:10.1093/carcin/bgw070. https://pubmed.ncbi.nlm.nih.gov/27288692/
4. Li, J C, Zhu, X J, Ye, J H, Luo, D H, Zhong, W D. . [Bioinformatics-based analysis of the effect of general Transcription Factor IIH on prognosis of Prostate cancer]. In Zhonghua yi xue za zhi, 104, 1410-1417. doi:10.3760/cma.j.cn112137-20231228-01504. https://pubmed.ncbi.nlm.nih.gov/38644292/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen