C57BL/6JCya-Il31em1/Cya
Common Name:
Il31-KO
Product ID:
S-KO-18175
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Il31-KO
Strain ID
KOCMP-76399-Il31-B6J-VB
Gene Name
Product ID
S-KO-18175
Gene Alias
1700013B14Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il31em1/Cya mice (Catalog S-KO-18175) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031389
NCBI RefSeq
NM_029594.2
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Il31, a cytokine, is mainly secreted by immune cells like TH2 cells [2,3]. It is a key player in neuroimmune communication, participating in multiple pathophysiological processes of atopic disorders, especially in driving pruritus in atopic dermatitis (AD) [2]. It exerts its effects through activation of the heterodimeric receptor IL31 receptor A (IL31RA)/Oncostatin M receptor (OSMRβ), which is expressed on dorsal root ganglia neurons, epithelial cells, and innate immune cells [2].
In a mouse model of house dust mite (HDM)-induced allergic dermatitis, Il31 -deficient mice showed less HDM dermatitis-associated scratching, consistent with Il31's role as a pruritogen [1]. However, Il31 deficiency increased the number and proportion of cutaneous type 2 cytokine-producing CD4+ T cells and serum IgE in response to HDM, and selectively enriched Il4ra+ monocytes and macrophages capable of fueling a feedforward type 2 inflammatory loop in HDM dermatitis skin [1]. This indicates that IL-31 is not strictly a pro-inflammatory cytokine but an immunoregulatory factor that limits the magnitude of type 2 inflammatory responses in the skin [1].
In conclusion, Il31 is a crucial cytokine in the context of neuroimmune-mediated itch and inflammation, especially in AD. Mouse models, such as the Il31-deficient mice, have been instrumental in revealing its dual role as a pruritogen and an immunoregulatory factor in allergic dermatitis, contributing to our understanding of the complex pathophysiology of AD and potentially other pruritic skin diseases.
References:
1. Fassett, Marlys S, Braz, Joao M, Castellanos, Carlos A, Basbaum, Allan I, Ansel, K Mark. 2023. IL-31-dependent neurogenic inflammation restrains cutaneous type 2 immune response in allergic dermatitis. In Science immunology, 8, eabi6887. doi:10.1126/sciimmunol.abi6887. https://pubmed.ncbi.nlm.nih.gov/37831760/
2. Datsi, Angeliki, Steinhoff, Martin, Ahmad, Fareed, Alam, Majid, Buddenkotte, Joerg. 2021. Interleukin-31: The "itchy" cytokine in inflammation and therapy. In Allergy, 76, 2982-2997. doi:10.1111/all.14791. https://pubmed.ncbi.nlm.nih.gov/33629401/
3. Cevikbas, Ferda, Wang, Xidao, Akiyama, Tasuku, Basbaum, Allan, Steinhoff, Martin. 2013. A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1. In The Journal of allergy and clinical immunology, 133, 448-60. doi:10.1016/j.jaci.2013.10.048. https://pubmed.ncbi.nlm.nih.gov/24373353/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen