C57BL/6JCya-Cmipem1/Cya
Common Name:
Cmip-KO
Product ID:
S-KO-18190
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cmip-KO
Strain ID
KOCMP-74440-Cmip-B6J-VA
Gene Name
Product ID
S-KO-18190
Gene Alias
4933407C03Rik; 5830471E12Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cmipem1/Cya mice (Catalog S-KO-18190) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000166750
NCBI RefSeq
NM_001163262
Target Region
Exon 8
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Cmip, also known as C-Maf-inducing protein, plays diverse roles in biological processes. It is involved in multiple signaling pathways, such as those related to T-cell activation, and has been associated with the pathophysiology of various diseases. Genetic models, especially knockout (KO) and conditional knockout (CKO) mouse models, can be valuable for studying its function [1-10].
In T-cells, CMIP acts as a negative regulator of signaling. Transgenic expression of CMIP drives T-cells towards a naïve phenotype, inhibiting the activation of Src kinases Fyn and Lck after CD3/CD28 costimulation and blocking the recruitment of LAT and lipid raft markers at the TCR engagement site [1].
In podocytes, overproduction of CMIP downregulates the Wilms tumor 1 suppressor gene (WT1) through preventing NF-κB-mediated transcriptional activation and targeting WT1 to proteasome degradation. Intravenous injection of Cmip-siRNA prevented the repression of Wt1 in a mouse model of lipopolysaccharides-induced proteinuria [2].
In non-alcoholic fatty liver disease (NAFLD), DNA methyltransferase (Dnmt) 1 and Tet methylcytosine dioxygenase 2 (Tet2)-mediated changes in Cmip methylation regulate the development of NAFLD by controlling the Gbp2-Pparγ-CD36 axis. Intravenous Cmip siRNA injection ameliorated NAFLD pathogenic features in ob/ob mice [3].
In conclusion, CMIP is involved in multiple biological functions including T-cell signaling regulation, WT1 expression control in podocytes, and the development of NAFLD. Studies using KO/CKO mouse models or siRNA-mediated knockdown in mice have provided insights into its role in these disease-related processes, highlighting its potential as a therapeutic target in T-cell-related disorders, podocyte diseases, and NAFLD.
References:
1. Oniszczuk, Julie, Sendeyo, Kelhia, Chhuon, Cerina, Sahali, Dil, Ollero, Mario. 2019. CMIP is a negative regulator of T cell signaling. In Cellular & molecular immunology, 17, 1026-1041. doi:10.1038/s41423-019-0266-5. https://pubmed.ncbi.nlm.nih.gov/31395948/
2. Zhang, Shao-Yu, Fan, Qingfeng, Moktefi, Anissa, Sahali, Dil, Henique, Carole. . CMIP interacts with WT1 and targets it on the proteasome degradation pathway. In Clinical and translational medicine, 11, e460. doi:10.1002/ctm2.460. https://pubmed.ncbi.nlm.nih.gov/34323419/
3. Lee, Jangho, Song, Ji-Hye, Park, Jae-Ho, Hwang, Jin-Taek, Choi, Hyo-Kyoung. 2023. Dnmt1/Tet2-mediated changes in Cmip methylation regulate the development of nonalcoholic fatty liver disease by controlling the Gbp2-Pparγ-CD36 axis. In Experimental & molecular medicine, 55, 143-157. doi:10.1038/s12276-022-00919-5. https://pubmed.ncbi.nlm.nih.gov/36609599/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen