C57BL/6JCya-Hnrnph1em1/Cya
Common Name:
Hnrnph1-KO
Product ID:
S-KO-18278
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hnrnph1-KO
Strain ID
KOCMP-59013-Hnrnph1-B6J-VB
Gene Name
Product ID
S-KO-18278
Gene Alias
E430005G16Rik; Hnrnph; Hnrph1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hnrnph1em1/Cya mice (Catalog S-KO-18278) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109142
NCBI RefSeq
NM_001362523
Target Region
Exon 4~6
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Hnrnph1, encoding heterogeneous nuclear ribonucleoprotein H1, is an RNA-binding protein involved in multiple RNA metabolic processes. It participates in alternative splicing, a crucial process for gene expression regulation, and is associated with pathways related to germ cell development, mitochondrial homeostasis, and stress responses. Its study through genetic models like knockout mouse models can help reveal its in-vivo functions [1,2].
Conditional knockout of Hnrnph1 in spermatogenic cells leads to abnormal splicing events, affecting meiosis-related genes and germ-Sertoli cell communication, ultimately causing male sterility. Hnrnph1-deficient oocytes also show defective synapsis and cell-cell junctions, leading to female infertility [1]. In mice, conditional knockout of Hnrnph1 in Sertoli cells compromises blood-testis barrier function, delays meiotic progression, increases germ cell apoptosis, and causes infertility [4]. Additionally, in 293T cells, knockdown and over-expression of hnRNPH1 proteins show that hnRNPH1 inhibits the replication of H1N1 and H9N2 influenza viruses by restraining viral polymerase activity [3].
In conclusion, Hnrnph1 is essential for germ cell development and male fertility as demonstrated by KO/CKO mouse models. It also plays a role in inhibiting influenza virus replication. These findings contribute to understanding the biological functions of Hnrnph1 in reproductive processes and antiviral defense mechanisms.
References:
1. Feng, Shenglei, Li, Jinmei, Wen, Hui, Wang, Xiaoli, Yuan, Shuiqiao. 2022. hnRNPH1 recruits PTBP2 and SRSF3 to modulate alternative splicing in germ cells. In Nature communications, 13, 3588. doi:10.1038/s41467-022-31364-7. https://pubmed.ncbi.nlm.nih.gov/35739118/
2. Zhao, Lili, Zou, Xiaotian, Deng, Jiaqiang, Zhao, Xudong, Zou, Fangdong. 2024. hnRNPH1 maintains mitochondrial homeostasis by establishing NRF1/DRP1 retrograde signaling under mitochondrial stress. In Cell death and differentiation, 32, 118-133. doi:10.1038/s41418-024-01331-4. https://pubmed.ncbi.nlm.nih.gov/38898233/
3. Xue, Ruixue, Bao, Danqi, Ma, Tianxin, Li, Zejun, Liu, Qinfang. 2024. hnRNPH1 Inhibits Influenza Virus Replication by Binding Viral RNA. In Microorganisms, 13, . doi:10.3390/microorganisms13010024. https://pubmed.ncbi.nlm.nih.gov/39858792/
4. Feng, Shenglei, Wen, Hui, Liu, Kuan, Wang, Xiaoli, Yuan, Shuiqiao. 2023. hnRNPH1 establishes Sertoli-germ cell crosstalk through cooperation with PTBP1 and AR, and is essential for male fertility in mice. In Development (Cambridge, England), 150, . doi:10.1242/dev.201040. https://pubmed.ncbi.nlm.nih.gov/36718792/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen