C57BL/6JCya-Plcb4em1/Cya
Common Name:
Plcb4-KO
Product ID:
S-KO-18348
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Plcb4-KO
Strain ID
KOCMP-18798-Plcb4-B6J-VB
Gene Name
Product ID
S-KO-18348
Gene Alias
A930039J07Rik; C230058B11Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Plcb4em1/Cya mice (Catalog S-KO-18348) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000110109
NCBI RefSeq
NM_013829
Target Region
Exon 7
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Plcb4, encoding phospholipase C β4, is a membrane-associated enzyme. It is a direct signaling effector of the endothelin receptor type A (EDNRA)-Gq/11 pathway, which is crucial for establishing the identity of neural crest cells (NCCs) that form lower jaw and middle ear structures. PLCB4 is also involved in regulating several cellular behaviors like cell motility, growth, transformation, and differentiation [3].
In mice, two major subpopulations of Purkinje neurons are identified by the expression of Aldoc and Plcb4. Plcb4+ Purkinje neurons exhibit robust plasticity of gene expression in sensorimotor and learning-experienced mice. In vivo calcium imaging and optogenetic perturbation show their crucial role in associative learning. Knockout of Fgfr2 in Plcb4+ Purkinje neurons using CRISPR disrupts motor learning, revealing the importance of Plcb4+ Purkinje neurons in motor learning [1]. In pediatric acute myeloid leukemia, high PLCB4 expression is an independent risk factor of adverse overall survival and event-free survival, and is significantly higher in relapsed patients [2]. In auriculocondylar syndrome 2 (ARCND2), missense mutations in PLCB4 exert a dominant-negative interference over EDNRA signaling. F0 mouse embryos modeling one PLCB4 variant have facial defects similar to hypomorphic Ednra mouse models [3].
In conclusion, Plcb4 is important in multiple biological processes. Its role in motor learning through Plcb4+ Purkinje neurons in mice is significant. Also, its abnormal expression is associated with diseases like pediatric acute myeloid leukemia and ARCND2. Studies using gene knockout models in mice have provided valuable insights into the functions of Plcb4 in these biological processes and disease conditions.
References:
1. Chen, Xiaoying, Du, Yanhua, Broussard, Gerard Joey, Zhang, Xiaoqing, Bonni, Azad. 2022. Transcriptomic mapping uncovers Purkinje neuron plasticity driving learning. In Nature, 605, 722-727. doi:10.1038/s41586-022-04711-3. https://pubmed.ncbi.nlm.nih.gov/35545673/
2. Wu, Shiwen, Zhang, Wei, Shen, Dongqin, Lu, Jianle, Zhao, Li. 2019. PLCB4 upregulation is associated with unfavorable prognosis in pediatric acute myeloid leukemia. In Oncology letters, 18, 6057-6065. doi:10.3892/ol.2019.10921. https://pubmed.ncbi.nlm.nih.gov/31788080/
3. Kanai, Stanley M, Heffner, Caleb, Cox, Timothy C, Murray, Stephen A, Clouthier, David E. 2022. Auriculocondylar syndrome 2 results from the dominant-negative action of PLCB4 variants. In Disease models & mechanisms, 15, . doi:10.1242/dmm.049320. https://pubmed.ncbi.nlm.nih.gov/35284927/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen