C57BL/6JCya-S1pr1em1/Cya
Common Name:
S1pr1-KO
Product ID:
S-KO-18447
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
S1pr1-KO
Strain ID
KOCMP-13609-S1pr1-B6J-VA
Gene Name
Product ID
S-KO-18447
Gene Alias
Edg1; Lpb1; S1p; S1p1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-S1pr1em1/Cya mice (Catalog S-KO-18447) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000055676
NCBI RefSeq
NM_007901
Target Region
Exon 2
Size of Effective Region
~3.9 kb
Detailed Document
Overview of Gene Research
S1pr1, short for sphingosine-1-phosphate receptor 1, is a G-protein coupled receptor for sphingosine 1-phosphate (S1P). It belongs to the sphingosine-1-phosphate receptor subfamily and is involved in multiple biological processes. S1pr1 plays crucial roles in regulating endothelial cell cytoskeletal structure, cell migration, immunomodulation, vasculogenesis during embryogenesis, and T cell egress [3].
In the context of osteoimmunology, S1P-S1PR1 signaling is a key regulator. Deletion or inhibition of this signaling axis can affect both bone remodeling directly by targeting osteoclastogenesis and osteogenesis, and indirectly by modulating the function and polarization of inflammatory cells in both adaptive and innate immune systems. This indicates its potential role in maintaining normal bone turnover and its implication in bone-related diseases like rheumatoid arthritis, spondyloarthritis, and periodontitis [1]. In ovarian cancer, S1PR1 deletion inhibits cell proliferation and migration, promotes cell senescence, and sensitizes cells to cisplatin chemotherapy, suggesting its role in cancer cell regulation through the PDK1-LATS1/2-YAP pathway [2]. In lung adenocarcinoma, S1PR1 expression is downregulated, and overexpression inhibits the malignant functions of cancer cells through the p-STAT1/miR-30c-5p/FOXA1 pathway, being positively correlated with prognosis [4]. Also, Sphk1/S1P/S1PR1 pathway differentially modulates the alloreactivity of CD4+ and CD8+ T cells, and inhibition of Sphk1 or S1PR1 can attenuate acute GVHD while retaining the graft-versus-leukemia effect [5].
In conclusion, S1pr1 is essential in various biological processes and disease conditions. Model-based research, especially gene knockout studies, has revealed its significance in bone remodeling, cancer cell regulation, and immune responses. Understanding S1pr1 can provide insights into the mechanisms of diseases such as bone-related disorders, ovarian and lung cancers, and graft-versus-host disease, potentially offering new therapeutic targets.
References:
1. Xiao, Lan, Zhou, Yinghong, Friis, Thor, Beagley, Kenneth, Xiao, Yin. 2019. S1P-S1PR1 Signaling: the "Sphinx" in Osteoimmunology. In Frontiers in immunology, 10, 1409. doi:10.3389/fimmu.2019.01409. https://pubmed.ncbi.nlm.nih.gov/31293578/
2. Tao, Yi-Ping, Zhu, Heng-Yan, Shi, Qian-Yuan, Cheng, Shu-Qun, Pan, Wei-Wei. 2023. S1PR1 regulates ovarian cancer cell senescence through the PDK1-LATS1/2-YAP pathway. In Oncogene, 42, 3491-3502. doi:10.1038/s41388-023-02853-w. https://pubmed.ncbi.nlm.nih.gov/37828220/
3. Anu, B, Namitha, N N, Harikumar, K B. 2021. S1PR1 signaling in cancer: A current perspective. In Advances in protein chemistry and structural biology, 125, 259-274. doi:10.1016/bs.apcsb.2020.12.006. https://pubmed.ncbi.nlm.nih.gov/33931142/
4. Chai, Yanfei, Xiang, Hong, Ma, Yuchao, Jin, Longyu, Lu, Hongwei. 2024. S1PR1 suppresses lung adenocarcinoma progression through p-STAT1/miR-30c-5 p/FOXA1 pathway. In Journal of experimental & clinical cancer research : CR, 43, 304. doi:10.1186/s13046-024-03230-5. https://pubmed.ncbi.nlm.nih.gov/39551792/
5. Tian, Linlu, Wu, Yongxia, Choi, Hee-Jin, Ogretmen, Besim, Yu, Xue-Zhong. 2022. S1P/S1PR1 signaling differentially regulates the allogeneic response of CD4 and CD8 T cells by modulating mitochondrial fission. In Cellular & molecular immunology, 19, 1235-1250. doi:10.1038/s41423-022-00921-x. https://pubmed.ncbi.nlm.nih.gov/36071219/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen