C57BL/6JCya-Flt3lem1/Cya
Common Name
Flt3l-KO
Product ID
S-KO-18516
Backgroud
C57BL/6JCya
Strain ID
KOCMP-14256-Flt3l-B6J-VA
When using this mouse strain in a publication, please cite “Flt3l-KO Mouse (Catalog S-KO-18516) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Flt3l-KO
Strain ID
KOCMP-14256-Flt3l-B6J-VA
Gene Name
Product ID
S-KO-18516
Gene Alias
Flt3lg, Ly72L
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000146760
NCBI RefSeq
NM_013520
Target Region
Exon 5~6
Size of Effective Region
~1.9 kb
Overview of Gene Research
Flt3l, also known as FMS-related tyrosine kinase 3 ligand and encoded by FLT3LG, is a hematopoietic factor. It acts on Flt3-expressing multipotent progenitors and common lymphoid progenitors. The Flt3/Flt3L axis is crucial for the generation of conventional DCs (cDCs) and plasmacytoid DCs (pDCs), and Flt3 signaling through PI3K and mTOR may affect mature DC function [2].
In mice, Flt3l is essential for the development of natural killer (NK) cells, B cells, and dendritic cells (DCs) [1]. In humans, three individuals homozygous for a loss-of-function FLT3LG variant had hypoplastic bone marrow, low levels of hematopoietic progenitors, and low counts of B cells, monocytes, and DCs in the blood. Unlike in mice, human Flt3L is required for monocyte development but not NK cell development [1]. Maternal low-fiber diet in mice reduced neonatal intestinal epithelial cell secretion of Flt3L, impairing pDC hematopoiesis and increasing LRI severity in offspring [3].
In conclusion, Flt3l is vital for the development of multiple hematopoietic lineages in both mice and humans, with some differences between species. Mouse models with loss-of-function of Flt3l have revealed its role in immune cell development and responses, such as in relation to respiratory infections, which may have implications for understanding and treating related human diseases.
References：
1. Momenilandi, Mana, Lévy, Romain, Sobrino, Steicy, Casanova, Jean-Laurent, Béziat, Vivien. 2024. FLT3L governs the development of partially overlapping hematopoietic lineages in humans and mice. In Cell, 187, 2817-2837.e31. doi:10.1016/j.cell.2024.04.009. https://pubmed.ncbi.nlm.nih.gov/38701783/
2. Cueto, Francisco J, Sancho, David. 2021. The Flt3L/Flt3 Axis in Dendritic Cell Biology and Cancer Immunotherapy. In Cancers, 13, . doi:10.3390/cancers13071525. https://pubmed.ncbi.nlm.nih.gov/33810248/
3. Sikder, Md Al Amin, Rashid, Ridwan B, Ahmed, Tufael, Dennis, Paul G, Phipps, Simon. 2023. Maternal diet modulates the infant microbiome and intestinal Flt3L necessary for dendritic cell development and immunity to respiratory infection. In Immunity, 56, 1098-1114.e10. doi:10.1016/j.immuni.2023.03.002. https://pubmed.ncbi.nlm.nih.gov/37003256/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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