C57BL/6JCya-Wdfy4em1/Cya
Common Name:
Wdfy4-KO
Product ID:
S-KO-18545
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Wdfy4-KO
Strain ID
KOCMP-545030-Wdfy4-B6J-VB
Gene Name
Product ID
S-KO-18545
Gene Alias
Gm18810
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wdfy4em1/Cya mice (Catalog S-KO-18545) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000061753
NCBI RefSeq
NM_001146022
Target Region
Exon 5~6
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
WDFY4, also known as WD repeat- and FYVE domain-containing protein 4, is a member of the BEACH (Beige and Chediak-Higashi) domain-containing family. It plays essential roles in the immune system, particularly in the cross-presentation of classic dendritic cells, reactive oxygen species-induced apoptosis of CD8+ T cells, and non-canonical autophagic activity in B cells [3]. Its functions are associated with multiple immune-related pathways and are of great biological importance in the context of immune responses and disease development. Genetic models, such as knockout (KO) mouse models, have been crucial in studying its functions.
In KO mouse models, Wdfy4-/-mice showed normal lymphoid and non-lymphoid cDC1 populations but failed to prime virus-specific CD8+ T cells in vivo or induce tumor rejection, indicating its requirement for cross-presentation in antiviral and antitumor immunity [1]. In NOD mice, inactivation of Wdfy4 using Nuclease technology led to amelioration of autoimmune diabetes and insulitis, as cDC1s were unable to cross-present cell-associated antigens to prime CD8+ T cells [2]. Lack of Wdfy4 in mice also aggravated ovalbumin-induced asthma via enhanced Th2 cell differentiation [4], reduced the number of CD8+ T cells via reactive oxygen species-induced apoptosis [5], and led to a decrease in total B cells and several B cell subpopulations, alleviating SLE phenotypes [6].
In conclusion, WDFY4 is essential for cross-presentation in the immune response, influencing antiviral, antitumor, and autoimmune disease processes. Gene knockout mouse models have been instrumental in revealing its role in these disease areas, providing insights into the mechanisms underlying immune-related diseases and potentially offering new targets for treatment.
References:
1. Theisen, Derek J, Davidson, Jesse T, Briseño, Carlos G, Murphy, Theresa L, Murphy, Kenneth M. . WDFY4 is required for cross-presentation in response to viral and tumor antigens. In Science (New York, N.Y.), 362, 694-699. doi:10.1126/science.aat5030. https://pubmed.ncbi.nlm.nih.gov/30409884/
2. Ferris, Stephen T, Liu, Tiantian, Chen, Jing, Murphy, Theresa L, Murphy, Kenneth M. 2023. WDFY4 deficiency in NOD mice ameliorates autoimmune diabetes and insulitis. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2219956120. doi:10.1073/pnas.2219956120. https://pubmed.ncbi.nlm.nih.gov/36940342/
3. Lyu, Xia, Lamb, Janine A, Chinoy, Hector. . The clinical relevance of WDFY4 in autoimmune diseases in diverse ancestral populations. In Rheumatology (Oxford, England), 63, 3255-3262. doi:10.1093/rheumatology/keae183. https://pubmed.ncbi.nlm.nih.gov/38507703/
4. Li, Yan, Wang, Anran, Long, Feng, Li, Jiangxia, Liu, Qiji. 2021. Lack of WDFY4 Aggravates Ovalbumin-Induced Asthma via Enhanced Th2 Cell Differentiation. In International archives of allergy and immunology, 182, 1089-1096. doi:10.1159/000516970. https://pubmed.ncbi.nlm.nih.gov/34425575/
5. Li, Yan, Li, Jiangxia, Yuan, Qianqian, Sun, Wenjie, Liu, Qiji. 2021. Deficiency in WDFY4 reduces the number of CD8+ T cells via reactive oxygen species-induced apoptosis. In Molecular immunology, 139, 131-138. doi:10.1016/j.molimm.2021.08.022. https://pubmed.ncbi.nlm.nih.gov/34482201/
6. Yuan, Qianqian, Li, Yan, Li, Jiangxia, Sun, Wenjie, Liu, Qiji. 2018. WDFY4 Is Involved in Symptoms of Systemic Lupus Erythematosus by Modulating B Cell Fate via Noncanonical Autophagy. In Journal of immunology (Baltimore, Md. : 1950), 201, 2570-2578. doi:10.4049/jimmunol.1800399. https://pubmed.ncbi.nlm.nih.gov/30257884/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen