C57BL/6JCya-Zfp42em1/Cya
Common Name:
Zfp42-KO
Product ID:
S-KO-18557
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfp42-KO
Strain ID
KOCMP-22702-Zfp42-B6J-VA
Gene Name
Product ID
S-KO-18557
Gene Alias
Rex-1; Rex1; Zfp-42
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp42em1/Cya mice (Catalog S-KO-18557) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000082120
NCBI RefSeq
NM_009556
Target Region
Exon 4
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Zfp42, also known as Rex1 (reduced expression gene 1), is a zinc-finger protein that has been associated with pluripotency/multipotency in embryonic stem cells. It is involved in various biological processes such as early embryogenesis, spermatogenesis, and may play a role in epigenetic regulation, including genomic imprinting [3].
In gene-knockout mouse models, Rex1(-/-) male mice show an age-associated decrease in sperm counts, abnormal sperm morphology, and mild testicular atrophy, indicating its importance in testicular function [4]. In embryonic stem cell differentiation, disruption of the Rex1 gene enhanced the expression of ectoderm, mesoderm, and endoderm markers, suggesting it acts to reduce retinoic-acid-induced differentiation [5]. In the context of glaucoma, in vitro experiments showed that Zfp42 reduces the damage of retinal ganglion cells by up-regulating the expression of MARK2 [1]. In colorectal cancer, CPNE7 interacts with NONO to initiate Zfp42 transcription, promoting tumorigenesis, and Zfp42 knockdown inhibits tumor cell proliferation and migration while promoting apoptosis [2].
In summary, Zfp42 is involved in multiple biological processes including stem cell-related functions, testicular development, and disease-related processes such as glaucoma and colorectal cancer. The use of gene-knockout mouse models has been crucial in revealing its role in these areas, helping to understand the underlying mechanisms and potentially providing new therapeutic targets for related diseases.
References:
1. Yin, Yuan, Wu, Shuai, Niu, Lingzhi, Huang, Shiwei. 2022. A ZFP42/MARK2 regulatory network reduces the damage of retinal ganglion cells in glaucoma: a study based on GEO dataset and in vitro experiments. In Apoptosis : an international journal on programmed cell death, 27, 1049-1059. doi:10.1007/s10495-022-01746-9. https://pubmed.ncbi.nlm.nih.gov/36131186/
2. Zhao, Liangbo, Sun, Xiao, Hou, Chenying, Jin, Shuiling, Liu, Benyu. 2024. CPNE7 promotes colorectal tumorigenesis by interacting with NONO to initiate ZFP42 transcription. In Cell death & disease, 15, 896. doi:10.1038/s41419-024-07288-z. https://pubmed.ncbi.nlm.nih.gov/39695095/
3. Kim, Jeong Do, Kim, Hana, Ekram, Muhammad B, Faulk, Christopher, Kim, Joomyeong. 2011. Rex1/Zfp42 as an epigenetic regulator for genomic imprinting. In Human molecular genetics, 20, 1353-62. doi:10.1093/hmg/ddr017. https://pubmed.ncbi.nlm.nih.gov/21233130/
4. Rezende, Naira C, Lee, Mi-Young, Monette, Sébastien, Lu, Ailan, Gudas, Lorraine J. 2011. Rex1 (Zfp42) null mice show impaired testicular function, abnormal testis morphology, and aberrant gene expression. In Developmental biology, 356, 370-82. doi:10.1016/j.ydbio.2011.05.664. https://pubmed.ncbi.nlm.nih.gov/21641340/
5. Scotland, Kymora B, Chen, Siming, Sylvester, Renia, Gudas, Lorraine J. . Analysis of Rex1 (zfp42) function in embryonic stem cell differentiation. In Developmental dynamics : an official publication of the American Association of Anatomists, 238, 1863-77. doi:10.1002/dvdy.22037. https://pubmed.ncbi.nlm.nih.gov/19618472/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen