C57BL/6JCya-Pus7em1/Cya
Common Name:
Pus7-KO
Product ID:
S-KO-18768
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pus7-KO
Strain ID
KOCMP-78697-Pus7-B6J-VA
Gene Name
Product ID
S-KO-18768
Gene Alias
C330017I15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pus7em1/Cya mice (Catalog S-KO-18768) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000131992
NCBI RefSeq
NM_001289781
Target Region
Exon 5
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Pus7, known as Pseudouridine synthase 7, is an enzyme crucial for pseudouridylation, a post-transcriptional RNA modification. Pseudouridine is the most frequent epitranscriptomic modification, influencing RNA function and gene expression [1,2,5,7,8]. Pus7 is involved in various biological processes such as translational control, especially in stem cells where it modifies tRNA-derived fragments to impact stem cell commitment [5]. It is also associated with multiple disease-related pathways, making it an important target for understanding disease mechanisms.
In glioblastoma, high Pus7 expression is associated with worse patient survival, and its expression and catalytic activity are required for glioblastoma stem cell tumorigenesis. Inhibition of Pus7 suppresses tRNA pseudouridylation, tumorigenesis, and extends the lifespan of tumor-bearing mice [1,4].
In gastric cancer, Pus7 is reduced in tumor tissues. It inhibits gastric cancer cell proliferation and tumor growth by enhancing the translation efficiency of ALKBH3 mRNA through pseudouridylation [2].
In colorectal cancer, Pus7 promotes cell proliferation by directly stabilizing SIRT1 to activate the Wnt/β-catenin pathway [3]. Also, HSP90-dependent Pus7 overexpression facilitates CRC cell metastasis by regulating LASP1 abundance [10].
In human patients, Pus7 deficiency causes a neurodevelopmental phenotype due to dysregulated protein translation [6]. Pan-cancer analysis shows Pus7 is overexpressed in most malignancies, contributing to poor prognosis in several cancer types, and is a potential biomarker and therapeutic target for osteosarcoma [7]. It also affects pre-mRNA processing by co-transcriptional pseudouridylation [8] and is involved in host-SARS-CoV-2 RNA interactions [9].
In conclusion, Pus7 plays essential roles in translational control and is closely associated with various diseases, especially cancer. The use of gene-knockout or conditional-knockout models in research has significantly contributed to understanding how Pus7 functions in disease-related biological processes, providing potential therapeutic strategies for glioblastoma, gastric cancer, colorectal cancer, and other malignancies, as well as insights into neurodevelopmental disorders.
References：
1. Cui, Qi, Yin, Kailin, Zhang, Xiaoting, Yi, Chengqi, Shi, Yanhong. 2021. Targeting PUS7 suppresses tRNA pseudouridylation and glioblastoma tumorigenesis. In Nature cancer, 2, 932-949. doi:10.1038/s43018-021-00238-0. https://pubmed.ncbi.nlm.nih.gov/35121864/
2. Chang, Yongxia, Jin, Hao, Cui, Yun, Xie, Shanshan, Zhou, Tianhua. . PUS7-dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression. In Clinical and translational medicine, 14, e1811. doi:10.1002/ctm2.1811. https://pubmed.ncbi.nlm.nih.gov/39175405/
3. Zhang, Qi, Fei, Sujuan, Zhao, Yanchao, Lu, Lili, Chen, Weichang. 2022. PUS7 promotes the proliferation of colorectal cancer cells by directly stabilizing SIRT1 to activate the Wnt/β-catenin pathway. In Molecular carcinogenesis, 62, 160-173. doi:10.1002/mc.23473. https://pubmed.ncbi.nlm.nih.gov/36222184/
4. Zhang, Daniel Y, Ming, Guo-Li, Song, Hongjun. 2021. PUS7: a targetable epitranscriptomic regulator of glioblastoma growth. In Trends in pharmacological sciences, 42, 976-978. doi:10.1016/j.tips.2021.10.002. https://pubmed.ncbi.nlm.nih.gov/34657723/
5. Guzzi, Nicola, Cieśla, Maciej, Ngoc, Phuong Cao Thi, Hsieh, Andrew C, Bellodi, Cristian. 2018. Pseudouridylation of tRNA-Derived Fragments Steers Translational Control in Stem Cells. In Cell, 173, 1204-1216.e26. doi:10.1016/j.cell.2018.03.008. https://pubmed.ncbi.nlm.nih.gov/29628141/
6. Han, Sangwoo T, Kim, Andrew C, Garcia, Karolyn, Malicdan, May C, Tifft, Cynthia J. 2022. PUS7 deficiency in human patients causes profound neurodevelopmental phenotype by dysregulating protein translation. In Molecular genetics and metabolism, 135, 221-229. doi:10.1016/j.ymgme.2022.01.103. https://pubmed.ncbi.nlm.nih.gov/35144859/
7. Dong, Baokang, Wang, Binqi, Fan, Meng, Zhang, Jingyu, Zhao, Ziqin. 2024. Comprehensive analysis to identify PUS7 as a prognostic biomarker from pan-cancer analysis to osteosarcoma validation. In Aging, 16, 9188-9203. doi:10.18632/aging.205863. https://pubmed.ncbi.nlm.nih.gov/38819212/
8. Martinez, Nicole M, Su, Amanda, Burns, Margaret C, Yeo, Gene W, Gilbert, Wendy V. 2022. Pseudouridine synthases modify human pre-mRNA co-transcriptionally and affect pre-mRNA processing. In Molecular cell, 82, 645-659.e9. doi:10.1016/j.molcel.2021.12.023. https://pubmed.ncbi.nlm.nih.gov/35051350/
9. Giambruno, Roberto, Zacco, Elsa, Ugolini, Camilla, Tartaglia, Gian Gaetano, Nicassio, Francesco. 2023. Unveiling the role of PUS7-mediated pseudouridylation in host protein interactions specific for the SARS-CoV-2 RNA genome. In Molecular therapy. Nucleic acids, 34, 102052. doi:10.1016/j.omtn.2023.102052. https://pubmed.ncbi.nlm.nih.gov/38028201/
10. Song, Dan, Guo, Ming, Xu, Shuai, Wang, Shiqi, Zhao, Qingchuan. 2021. HSP90-dependent PUS7 overexpression facilitates the metastasis of colorectal cancer cells by regulating LASP1 abundance. In Journal of experimental & clinical cancer research : CR, 40, 170. doi:10.1186/s13046-021-01951-5. https://pubmed.ncbi.nlm.nih.gov/33990203/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen