C57BL/6JCya-Dcaf12em1/Cya
Common Name:
Dcaf12-KO
Product ID:
S-KO-18787
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Dcaf12-KO
Strain ID
KOCMP-68970-Dcaf12-B6J-VB
Gene Name
Product ID
S-KO-18787
Gene Alias
1500001L20Rik; 5830424K06Rik; Wdr40a
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dcaf12em1/Cya mice (Catalog S-KO-18787) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030145
NCBI RefSeq
NM_026893
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Dcaf12, short for Damaged DNA-binding protein-1 (DDB1)- and CUL4-associated factor 12, is a crucial protein. It functions as the substrate recognition component within the Cullin4-RING E3 ligase (CRL4) complex, identifying C-terminal double-glutamic acid degrons to regulate protein degradation through the ubiquitin proteasome system. It is involved in multiple biological pathways, such as apoptosis regulation and NF-κB activation, and is of great biological importance in processes like spermatogenesis, T -cell activation, and synaptic function [1,2,3]. Drosophila and mouse models have been valuable in studying its functions.
In mice, Dcaf12 knockout leads to fewer mature sperms in testes, along with elevated MOV10 and an imbalance in meiotic markers SCP3 and γ-H2AX, demonstrating its role in spermatogenesis. Activated Dcaf12 -deficient T cells show inappropriately stabilized MOV10 and increased levels of activated caspases, indicating its significance in T -cell activation [2]. In Drosophila, deletion of Dcaf12 impairs larval locomotion, arrests development, and affects neurotransmitter release, synaptic receptor levels, and homeostatic plasticity at larval neuromuscular junctions [3].
In summary, Dcaf12 is essential for various biological processes including spermatogenesis, T -cell activation, and synaptic function. Gene knockout models in mice and Drosophila have been instrumental in revealing its role in these processes, potentially providing insights into related human diseases such as male infertility and immune-related disorders.
References:
1. Righetto, Germanna Lima, Yin, Yanting, Duda, David M, Santhakumar, Vijayaratnam, Halabelian, Levon. 2024. Probing the CRL4DCAF12 interactions with MAGEA3 and CCT5 di-Glu C-terminal degrons. In PNAS nexus, 3, pgae153. doi:10.1093/pnasnexus/pgae153. https://pubmed.ncbi.nlm.nih.gov/38665159/
2. Lidak, Tomas, Baloghova, Nikol, Korinek, Vladimir, Kasparek, Petr, Cermak, Lukas. 2021. CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation. In International journal of molecular sciences, 22, . doi:10.3390/ijms22105394. https://pubmed.ncbi.nlm.nih.gov/34065512/
3. Patrón, Lilian A, Nagatomo, Kei, Eves, David Tyler, Rogers, Gregory C, Zinsmaier, Konrad E. 2019. Cul4 ubiquitin ligase cofactor DCAF12 promotes neurotransmitter release and homeostatic plasticity. In The Journal of cell biology, 218, 993-1010. doi:10.1083/jcb.201805099. https://pubmed.ncbi.nlm.nih.gov/30670470/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen