C57BL/6JCya-Barx1em1/Cya
Common Name:
Barx1-KO
Product ID:
S-KO-18831
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Barx1-KO
Strain ID
KOCMP-12022-Barx1-B6J-VA
Gene Name
Product ID
S-KO-18831
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Barx1em1/Cya mice (Catalog S-KO-18831) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021813
NCBI RefSeq
NM_007526
Target Region
Exon 2~4
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Barx1, a homeobox-containing gene, has diverse essential functions. During mouse embryonic development, it is expressed in molar tooth and stomach mesenchymal cells. In molar development, it instructs undetermined ectomesenchymal cells in the jaws to follow a multicuspid tooth developmental pathway. In the presumptive stomach mesenchyme, it attenuates Wnt signalling to allow stomach epithelium differentiation [3].
In disease-related studies, in non-small cell lung cancer (NSCLC), loss of ZFP36 leads to BARX1 upregulation, promoting cell proliferation, migration, and invasion by transactivating master oncogenes [1]. In osteosarcoma, BARX1 is highly expressed, and its downregulation suppresses cell proliferation and migration. It promotes these processes by inducing HSPA6 expression [2]. In lung adenocarcinoma, BARX1 represses FOXF1 expression and activates the Wnt/β-catenin signalling pathway to drive malignancy [4]. In gastrointestinal stromal tumor, BARX1 is expressed in indolent and micro-GISTs, and its expression pattern can predict relapse-free survival [5].
In conclusion, Barx1 plays crucial roles in embryonic development, especially in tooth and stomach formation. In disease, it is involved in the development and progression of various cancers including NSCLC, osteosarcoma, lung adenocarcinoma, and gastrointestinal stromal tumors. Studies using gene-related models like those in the referenced research help reveal these functions, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Zhang, Tongjia, Qiu, Lizhen, Cao, Jiashun, Li, Shuyan, Li, Kailong. 2023. ZFP36 loss-mediated BARX1 stabilization promotes malignant phenotypes by transactivating master oncogenes in NSCLC. In Cell death & disease, 14, 527. doi:10.1038/s41419-023-06044-z. https://pubmed.ncbi.nlm.nih.gov/37587140/
2. Huang, Xing, Wang, Zhenhua, Zhang, Jing, Han, Zhitao, Liu, Tielong. 2023. BARX1 promotes osteosarcoma cell proliferation and invasion by regulating HSPA6 expression. In Journal of orthopaedic surgery and research, 18, 211. doi:10.1186/s13018-023-03690-z. https://pubmed.ncbi.nlm.nih.gov/36927457/
3. Miletich, Isabelle, Buchner, Georg, Sharpe, Paul T. . Barx1 and evolutionary changes in feeding. In Journal of anatomy, 207, 619-22. doi:. https://pubmed.ncbi.nlm.nih.gov/16313395/
4. Guan, Xiaojiao, Liang, Jie, Xiang, Yifan, Li, Tian, Zhong, Xinwen. 2024. BARX1 repressed FOXF1 expression and activated Wnt/β-catenin signaling pathway to drive lung adenocarcinoma. In International journal of biological macromolecules, 261, 129717. doi:10.1016/j.ijbiomac.2024.129717. https://pubmed.ncbi.nlm.nih.gov/38290639/
5. Hemming, Matthew L, Coy, Shannon, Lin, Jia-Ren, Demetri, George D, Santagata, Sandro. 2021. HAND1 and BARX1 Act as Transcriptional and Anatomic Determinants of Malignancy in Gastrointestinal Stromal Tumor. In Clinical cancer research : an official journal of the American Association for Cancer Research, 27, 1706-1719. doi:10.1158/1078-0432.CCR-20-3538. https://pubmed.ncbi.nlm.nih.gov/33451979/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen