C57BL/6JCya-Wfdc18em1/Cya
Common Name:
Wfdc18-KO
Product ID:
S-KO-18849
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Wfdc18-KO
Strain ID
KOCMP-14038-Wfdc18-B6J-VB
Gene Name
Product ID
S-KO-18849
Gene Alias
Expi; Kal1; WDNM1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wfdc18em1/Cya mice (Catalog S-KO-18849) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001009
NCBI RefSeq
NM_007969
Target Region
Exon 2~3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Wfdc18, also known as Wdnm1 or Expi, belongs to the 'four-disulphide core' family of proteins and may have proteinase inhibiting capacity [6]. It has been associated with various biological processes, though its exact function is still being explored. Given its potential role as a proteinase inhibitor, it may be involved in processes related to tissue remodeling and cell-matrix interactions.
In sika deer, the WFDC18 gene was abundant at the ossification stage of antler development, indicating its possible role in this process [1]. In mouse mammary gland, WDNM1 (alias of Wfdc18) was predominantly expressed in the layer of secretory epithelial cells surrounding the lumen of mammary gland alveoli, and its expression was down-regulated during involution [4]. Also, in mouse mammary epithelial cells, EGF strongly inhibited the expression of WDNM1 [5]. In mouse models related to liver fibrosis, the extracellular proteinase inhibitor gene Expi (Wfdc18) within a 6 Mb interval on chromosome 11 was ranked as a strong candidate that may modulate the severity of liver fibrosis [2]. In an imiquimod-induced psoriasis model, sakuranetin upregulated Wfdc18, suggesting its role in epidermal homeostasis and skin barrier function [3].
In conclusion, Wfdc18 plays important roles in multiple biological processes such as antler ossification, mammary gland involution, liver fibrosis, and skin barrier function. Studies using mouse models, in particular, have been valuable in revealing these functions, providing insights into its potential significance in diseases related to these processes, including liver fibrosis and psoriasis.
References:
1. Zhang, Ranran, Dong, Yimeng, Xing, Xiumei. 2022. Comprehensive transcriptome analysis of sika deer antler using PacBio and Illumina sequencing. In Scientific reports, 12, 16161. doi:10.1038/s41598-022-20244-1. https://pubmed.ncbi.nlm.nih.gov/36171236/
2. Liebe, Roman, Hall, Rabea A, Williams, Robert W, Dooley, Steven, Lammert, Frank. 2013. Systems genetics of hepatocellular damage in vivo and in vitro: identification of a critical network on chromosome 11 in mouse. In Physiological genomics, 45, 931-9. doi:10.1152/physiolgenomics.00078.2013. https://pubmed.ncbi.nlm.nih.gov/23943854/
3. Chen, Yung-Chuan, Cheng, Yu-Ping, Liu, Chih-Yi, Lai, Chien-Yu, Guo, Jiun-Wen. 2025. Sakuranetin modulates IL-17A-related inflammation and enhances skin barrier function in an imiquimod-induced psoriasis model. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 186, 118035. doi:10.1016/j.biopha.2025.118035. https://pubmed.ncbi.nlm.nih.gov/40209307/
4. Baik, M, Hennighausen, L, Choi, Y. . In situ localization of WDNM1 and ferritin heavy chain gene expression in mammary gland. In Molecules and cells, 7, 448-50. doi:. https://pubmed.ncbi.nlm.nih.gov/9264037/
5. Lee, M, Hwang, I, Choi, Y, Lee, H B, Baik, M. . EGF inhibits expression of WDNM1 and sulfated glycoprotein-2 genes in mammary epithelial cells. In Biochemical and biophysical research communications, 240, 104-7. doi:. https://pubmed.ncbi.nlm.nih.gov/9367891/
6. Dear, T N, Kefford, R F. . The WDNM1 gene product is a novel member of the 'four-disulphide core' family of proteins. In Biochemical and biophysical research communications, 176, 247-54. doi:. https://pubmed.ncbi.nlm.nih.gov/2018519/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen