C57BL/6JCya-Prkacaem1/Cya
Common Name:
Prkaca-KO
Product ID:
S-KO-18863
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Prkaca-KO
Strain ID
KOCMP-18747-Prkaca-B6J-VA
Gene Name
Product ID
S-KO-18863
Gene Alias
PKCD; Pkaca
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prkacaem1/Cya mice (Catalog S-KO-18863) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000005606
NCBI RefSeq
NM_008854
Target Region
Exon 4~5
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Prkaca, which encodes the catalytic subunit α of protein kinase A (PKA), is a key player in cAMP-dependent protein kinase signaling. PKA, as a holoenzyme, consists of a regulatory subunit dimer and two catalytic subunits. When cAMP binds to the regulatory subunits, the catalytic subunits are released to phosphorylate downstream targets, thus propagating cAMP-responsive cell signaling events. This signaling pathway is involved in various biological processes, and Prkaca is of great biological importance in maintaining normal physiological functions [7].
In the context of diseases, Prkaca has been implicated in multiple conditions. Somatic mutations in Prkaca are the most frequently altered gene in cortisol-producing adenomas, and patients with these mutations often present with a more severe phenotype at a younger age [3]. Germline Prkaca amplification has been associated with primary pigmented nodular adrenocortical disease (PPNAD), a rare form of ACTH-independent Cushing's syndrome [2]. In fibrolamellar hepatocellular carcinoma, the DNAJB1-Prkaca fusion transcript is the oncogenic driver, and this fusion protein can be a target for peptide-based immunotherapy [1,4]. Also, somatic Prkaca mutations may be related to the accelerated development of aldosterone-and cortisol-producing adenomas during pregnancy [5]. Additionally, Prkaca-related, atrial defects-polydactyly-multiple congenital malformation syndrome is caused by disease-causing variants in Prkaca [6].
In conclusion, Prkaca is essential for cAMP-dependent protein kinase signaling, which is crucial for normal physiological functions. Its involvement in various diseases, such as adrenal tumors, PPNAD, and fibrolamellar hepatocellular carcinoma, has been revealed through studies. Understanding Prkaca's functions in these disease conditions can potentially provide new therapeutic strategies for these diseases.
References:
1. Bauer, Jens, Köhler, Natalie, Maringer, Yacine, Hailfinger, Stephan, Walz, Juliane S. 2022. The oncogenic fusion protein DNAJB1-PRKACA can be specifically targeted by peptide-based immunotherapy in fibrolamellar hepatocellular carcinoma. In Nature communications, 13, 6401. doi:10.1038/s41467-022-33746-3. https://pubmed.ncbi.nlm.nih.gov/36302754/
2. Yang, Wang-Rong, Liang, Xing-Huan, Qin, Ying-Fen, Liu, Yu-Ping, Luo, Zuo-Jie. . Germline PRKACA amplification-associated primary pigmented nodular adrenocortical disease: a case report and literature review. In Archives of endocrinology and metabolism, 68, e220491. doi:10.20945/2359-4292-2022-0491. https://pubmed.ncbi.nlm.nih.gov/37988664/
3. Dalmazi, G D, Beuschlein, F. 2016. PRKACA Mutations in Adrenal Adenomas: Genotype/Phenotype Correlations. In Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme, 49, 301-306. doi:10.1055/s-0042-120416. https://pubmed.ncbi.nlm.nih.gov/27871112/
4. Kirk, Allison M, Crawford, Jeremy Chase, Chou, Ching-Heng, Strome, Scott E, Thomas, Paul G. . DNAJB1-PRKACA fusion neoantigens elicit rare endogenous T cell responses that potentiate cell therapy for fibrolamellar carcinoma. In Cell reports. Medicine, 5, 101469. doi:10.1016/j.xcrm.2024.101469. https://pubmed.ncbi.nlm.nih.gov/38508137/
5. Lin, Jianfan, Li, Yufei, Huang, Zhenxing, Lu, Decheng, Luo, Zuojie. 2024. Rare correlation of somatic PRKACA mutations with pregnancy-associated aldosterone- and cortisol-producing adenomas: a case report and literature review. In BMC endocrine disorders, 24, 116. doi:10.1186/s12902-024-01645-x. https://pubmed.ncbi.nlm.nih.gov/39010034/
6. Sithambaram, Sivagamy, Jacob, Prince, Neethukrishna, Kausthubham, Shah, Hitesh, Girisha, Katta Mohan. 2024. PRKACA-related, atrial defects-polydactyly-multiple congenital malformation syndrome in an Indian patient. In American journal of medical genetics. Part A, 194, e63566. doi:10.1002/ajmg.a.63566. https://pubmed.ncbi.nlm.nih.gov/38357848/
7. Turnham, Rigney E, Scott, John D. 2015. Protein kinase A catalytic subunit isoform PRKACA; History, function and physiology. In Gene, 577, 101-8. doi:10.1016/j.gene.2015.11.052. https://pubmed.ncbi.nlm.nih.gov/26687711/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen