C57BL/6JCya-Mir125b-2em1/Cya
Common Name:
Mir125b-2-KO
Product ID:
S-KO-18878
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mir125b-2-KO
Strain ID
KOCMP-723952-Mir125b-2-B6J-VA
Gene Name
Product ID
S-KO-18878
Gene Alias
Mirn125b-2; mir-125b-2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir125b-2em1/Cya mice (Catalog S-KO-18878) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083538
NCBI RefSeq
NR_029540
Target Region
Exon 1
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Mir125b-2 is a microRNA that appears to have significant functions in the brain and in cancer-related processes. In the context of brain function, it may be involved in genomic imprinting-related pathways, which play a crucial role in parent-of-origin-specific gene expression. In cancer, it may be associated with tumor-suppressing pathways [1,2].
In mice, Mir125b-2m-/p-(knockout) mice showed impaired learning and memory, as well as anxiety, with these functions being hippocampus-dependent. Hippocampal granule cells from these mice had increased neuronal excitability, increased excitatory synaptic transmission, and decreased inhibitory synaptic transmission. The gene Glutamate ionotropic receptor NMDA type subunit 2A (Grin2a), a key regulator of synaptic plasticity, was physically bound by miR-125b-2 and upregulated in the hippocampus of Mir125b-2m-/p-mice. This indicates that Mir125b-2 plays a role in regulating hippocampal function and circuit in mice [1]. In luminal A breast cancer, the aryl hydrocarbon receptor (AhR) agonist aminoflavone (AF) upregulates miR125b-2-3p, which acts as a tumor suppressor. miR125b-2-3p mimic decreased the expression of stemness genes and inhibited proliferation, migration, and mammosphere formation in breast cancer cells, while the antagomiR125b-2-3p had the opposite effects [2].
In conclusion, Mir125b-2 has important functions in the brain, regulating hippocampal-dependent behaviors and synaptic transmission. In luminal A breast cancer, it may act as a tumor suppressor. The use of Mir125b-2 knockout mouse models has been instrumental in revealing its role in brain-related processes, and research in breast cancer cells has further expanded our understanding of its potential anti-cancer functions.
References:
1. Chou, Ming-Yi, Cao, Xuhui, Hou, Kuan-Chu, Lin, Shu-Wha, Huang, Hsien-Sung. 2023. Mir125b-2 imprinted in human but not mouse brain regulates hippocampal function and circuit in mice. In Communications biology, 6, 267. doi:10.1038/s42003-023-04655-y. https://pubmed.ncbi.nlm.nih.gov/36918719/
2. Mavingire, Nicole, Campbell, Petreena, Liu, Tiantian, Wang, Charles, Brantley, Eileen. 2022. Aminoflavone upregulates putative tumor suppressor miR-125b-2-3p to inhibit luminal A breast cancer stem cell-like properties. In Precision clinical medicine, 5, pbac008. doi:10.1093/pcmedi/pbac008. https://pubmed.ncbi.nlm.nih.gov/35694715/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen