C57BL/6JCya-Plscr1em1/Cya
Common Name:
Plscr1-KO
Product ID:
S-KO-18886
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Plscr1-KO
Strain ID
KOCMP-22038-Plscr1-B6J-VB
Gene Name
Product ID
S-KO-18886
Gene Alias
MmTRA1a; MmTRA1b; Nor1; Tra1; Tra1a; Tra1b; Tras1; Tras2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Plscr1em1/Cya mice (Catalog S-KO-18886) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000093801
NCBI RefSeq
NM_011636
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Plscr1, short for phospholipid scramblase 1, is a protein-coding gene with significant functions in multiple biological processes. It is involved in maintaining phospholipid distribution in cell membranes, and its expression can be induced by interferons (IFNs) [1,3,5,6,8]. PLSCR1 plays a crucial role in the host innate immunity against viral infections, and it has also been linked to cell apoptosis, cancer progression, and cell differentiation [1,2,4,5,6,7,9]. Genetic models, such as gene knockout mouse models, are valuable tools to study its functions.
In terms of viral infections, genome-wide CRISPR-Cas9 screens have identified PLSCR1 as a cell-autonomous restriction factor against live SARS-CoV-2 infection in human lung epithelia and hepatocytes. IFNγ-induced PLSCR1 restricts not only SARS-CoV-2 USA-WA1/2020 but also the Delta and Omicron lineages, and it acts on other highly pathogenic coronaviruses [1]. It interferes with the uptake of SARS-CoV-2 in both endocytic and TMPRSS2-dependent fusion routes, directly targeting SARS-CoV-2-containing vesicles to prevent spike-mediated fusion and viral escape [1]. Moreover, PLSCR1-knockout 36T-3 cells show significantly higher levels of human cytomegalovirus (HCMV) plaque formation and replication compared to parental cells, indicating that PLSCR1 negatively regulates HCMV replication [5]. In influenza A virus infection, Plscr1-/-mice are more susceptible to H1N1 SIV infection, suggesting its antiviral function in vivo [4].
In conclusion, PLSCR1 is a multifunctional gene playing essential roles in antiviral immunity, as demonstrated by gene-knockout models in the context of SARS-CoV-2, HCMV, and influenza A virus infections. It also has implications in other disease-related processes such as glaucoma-associated apoptosis of retinal ganglion cells, and the progression of basal-like breast cancer and acute myeloid leukemia [2,7,9]. The study of PLSCR1 using knockout models helps to understand its biological functions and provides insights into potential therapeutic strategies for related diseases.
References:
1. Xu, Dijin, Jiang, Weiqian, Wu, Lizhen, Wilen, Craig B, MacMicking, John D. 2023. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. In Nature, 619, 819-827. doi:10.1038/s41586-023-06322-y. https://pubmed.ncbi.nlm.nih.gov/37438530/
2. Luo, Jingyi, Lian, Qing, Zhu, Deliang, Lu, Lin, Zhao, Ling. 2022. PLSCR1 promotes apoptosis and clearance of retinal ganglion cells in glaucoma pathogenesis. In Genes & diseases, 10, 1564-1581. doi:10.1016/j.gendis.2022.05.036. https://pubmed.ncbi.nlm.nih.gov/37397520/
3. Li, Minghua. . Fortifying immunity: PLSCR1 picks battle against SARS-CoV-2. In Cell host & microbe, 31, 1417-1419. doi:10.1016/j.chom.2023.08.014. https://pubmed.ncbi.nlm.nih.gov/37708846/
4. Liu, Yueyue, Lin, Shuqian, Xie, Yunhui, Xu, Zhigang, Wu, Jiaqiang. 2022. ILDR1 promotes influenza A virus replication through binding to PLSCR1. In Scientific reports, 12, 8515. doi:10.1038/s41598-022-12598-3. https://pubmed.ncbi.nlm.nih.gov/35595813/
5. Sadanari, Hidetaka, Takemoto, Masaya, Ishida, Tomoki, Murayama, Tsugiya, Kusano, Shuichi. 2022. The Interferon-Inducible Human PLSCR1 Protein Is a Restriction Factor of Human Cytomegalovirus. In Microbiology spectrum, 10, e0134221. doi:10.1128/spectrum.01342-21. https://pubmed.ncbi.nlm.nih.gov/35138119/
6. Ma, Ruiyi, Zhang, Xinyi, Li, Ruonan, Lei, Xiaobo, Wang, Jianwei. 2025. PLSCR1 suppresses SARS-CoV-2 infection by downregulating cell surface ACE2. In Journal of virology, 99, e0208524. doi:10.1128/jvi.02085-24. https://pubmed.ncbi.nlm.nih.gov/39945535/
7. Huang, Panpan, Liao, Ruocen, Chen, Xingyu, Cao, Qianhua, Dong, Chenfang. 2020. Nuclear translocation of PLSCR1 activates STAT1 signaling in basal-like breast cancer. In Theranostics, 10, 4644-4658. doi:10.7150/thno.43150. https://pubmed.ncbi.nlm.nih.gov/32292520/
8. Le Pen, Jérémie, Paniccia, Gabrielle, Kinast, Volker, MacDonald, Margaret R, Rice, Charles M. 2024. A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist. In PLoS biology, 22, e3002767. doi:10.1371/journal.pbio.3002767. https://pubmed.ncbi.nlm.nih.gov/39316623/
9. Li, Hui, Xu, Jingyan, Zhou, Yuxin, Guo, Qinglong, Hui, Hui. 2017. PLSCR1/IP3R1/Ca2+ axis contributes to differentiation of primary AML cells induced by wogonoside. In Cell death & disease, 8, e2768. doi:10.1038/cddis.2017.175. https://pubmed.ncbi.nlm.nih.gov/28492556/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen