C57BL/6JCya-Plscr1em1/Cya
Common Name
Plscr1-KO
Product ID
S-KO-18886
Backgroud
C57BL/6JCya
Strain ID
KOCMP-22038-Plscr1-B6J-VB
When using this mouse strain in a publication, please cite “Plscr1-KO Mouse (Catalog S-KO-18886) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Plscr1-KO
Strain ID
KOCMP-22038-Plscr1-B6J-VB
Gene Name
Product ID
S-KO-18886
Gene Alias
MmTRA1a, MmTRA1b, Nor1, Tra1, Tra1a, Tra1b, Tras1, Tras2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000093801
NCBI RefSeq
NM_011636
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Overview of Gene Research
Plscr1, short for phospholipid scramblase 1, is a protein-coding gene with significant functions in multiple biological processes. It is involved in maintaining phospholipid distribution in cell membranes, and its expression can be induced by interferons (IFNs) [1,3,5,6,8]. PLSCR1 plays a crucial role in the host innate immunity against viral infections, and it has also been linked to cell apoptosis, cancer progression, and cell differentiation [1,2,4,5,6,7,9]. Genetic models, such as gene knockout mouse models, are valuable tools to study its functions.
In terms of viral infections, genome-wide CRISPR-Cas9 screens have identified PLSCR1 as a cell-autonomous restriction factor against live SARS-CoV-2 infection in human lung epithelia and hepatocytes. IFNγ-induced PLSCR1 restricts not only SARS-CoV-2 USA-WA1/2020 but also the Delta and Omicron lineages, and it acts on other highly pathogenic coronaviruses [1]. It interferes with the uptake of SARS-CoV-2 in both endocytic and TMPRSS2-dependent fusion routes, directly targeting SARS-CoV-2-containing vesicles to prevent spike-mediated fusion and viral escape [1]. Moreover, PLSCR1-knockout 36T-3 cells show significantly higher levels of human cytomegalovirus (HCMV) plaque formation and replication compared to parental cells, indicating that PLSCR1 negatively regulates HCMV replication [5]. In influenza A virus infection, Plscr1-/-mice are more susceptible to H1N1 SIV infection, suggesting its antiviral function in vivo [4].
In conclusion, PLSCR1 is a multifunctional gene playing essential roles in antiviral immunity, as demonstrated by gene-knockout models in the context of SARS-CoV-2, HCMV, and influenza A virus infections. It also has implications in other disease-related processes such as glaucoma-associated apoptosis of retinal ganglion cells, and the progression of basal-like breast cancer and acute myeloid leukemia [2,7,9]. The study of PLSCR1 using knockout models helps to understand its biological functions and provides insights into potential therapeutic strategies for related diseases.
References：
1. Xu, Dijin, Jiang, Weiqian, Wu, Lizhen, Wilen, Craig B, MacMicking, John D. 2023. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. In Nature, 619, 819-827. doi:10.1038/s41586-023-06322-y. https://pubmed.ncbi.nlm.nih.gov/37438530/
2. Luo, Jingyi, Lian, Qing, Zhu, Deliang, Lu, Lin, Zhao, Ling. 2022. PLSCR1 promotes apoptosis and clearance of retinal ganglion cells in glaucoma pathogenesis. In Genes & diseases, 10, 1564-1581. doi:10.1016/j.gendis.2022.05.036. https://pubmed.ncbi.nlm.nih.gov/37397520/
3. Li, Minghua. . Fortifying immunity: PLSCR1 picks battle against SARS-CoV-2. In Cell host & microbe, 31, 1417-1419. doi:10.1016/j.chom.2023.08.014. https://pubmed.ncbi.nlm.nih.gov/37708846/
4. Liu, Yueyue, Lin, Shuqian, Xie, Yunhui, Xu, Zhigang, Wu, Jiaqiang. 2022. ILDR1 promotes influenza A virus replication through binding to PLSCR1. In Scientific reports, 12, 8515. doi:10.1038/s41598-022-12598-3. https://pubmed.ncbi.nlm.nih.gov/35595813/
5. Sadanari, Hidetaka, Takemoto, Masaya, Ishida, Tomoki, Murayama, Tsugiya, Kusano, Shuichi. 2022. The Interferon-Inducible Human PLSCR1 Protein Is a Restriction Factor of Human Cytomegalovirus. In Microbiology spectrum, 10, e0134221. doi:10.1128/spectrum.01342-21. https://pubmed.ncbi.nlm.nih.gov/35138119/
6. Ma, Ruiyi, Zhang, Xinyi, Li, Ruonan, Lei, Xiaobo, Wang, Jianwei. 2025. PLSCR1 suppresses SARS-CoV-2 infection by downregulating cell surface ACE2. In Journal of virology, 99, e0208524. doi:10.1128/jvi.02085-24. https://pubmed.ncbi.nlm.nih.gov/39945535/
7. Huang, Panpan, Liao, Ruocen, Chen, Xingyu, Cao, Qianhua, Dong, Chenfang. 2020. Nuclear translocation of PLSCR1 activates STAT1 signaling in basal-like breast cancer. In Theranostics, 10, 4644-4658. doi:10.7150/thno.43150. https://pubmed.ncbi.nlm.nih.gov/32292520/
8. Le Pen, Jérémie, Paniccia, Gabrielle, Kinast, Volker, MacDonald, Margaret R, Rice, Charles M. 2024. A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist. In PLoS biology, 22, e3002767. doi:10.1371/journal.pbio.3002767. https://pubmed.ncbi.nlm.nih.gov/39316623/
9. Li, Hui, Xu, Jingyan, Zhou, Yuxin, Guo, Qinglong, Hui, Hui. 2017. PLSCR1/IP3R1/Ca2+ axis contributes to differentiation of primary AML cells induced by wogonoside. In Cell death & disease, 8, e2768. doi:10.1038/cddis.2017.175. https://pubmed.ncbi.nlm.nih.gov/28492556/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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