C57BL/6JCya-Ifitm1em1/Cya
Common Name:
Ifitm1-KO
Product ID:
S-KO-18921
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ifitm1-KO
Strain ID
KOCMP-68713-Ifitm1-B6J-VB
Gene Name
Product ID
S-KO-18921
Gene Alias
1110036C17Rik; DSPA2a; Mil-2; Mil2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ifitm1em1/Cya mice (Catalog S-KO-18921) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000106042
NCBI RefSeq
NM_026820
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Ifitm1, short for interferon (IFN)-induced transmembrane protein 1, is a member of the IFN-induced transmembrane protein family. It is involved in the innate immune response triggered by viral infection, playing a crucial role in cellular antiviral responses [2,5]. It has also been associated with functions in germ cell specification and immune functions [5].
Ifitm1 is highly expressed in multiple tumor tissues and cancer cell lines, and is an independent prognostic biomarker for certain tumor types like gallbladder carcinoma, esophageal adenocarcinoma, colorectal cancer, and gastric cancer. Overexpression of Ifitm1 promotes tumor cell proliferation, invasion, metastasis, angiogenesis, and therapeutic resistance [1]. In colorectal cancer, Apc mutation induces Ifitm1 expression, and Ifitm1high cells have reduced uptake of fibroblast extracellular vesicles (EVs), with inactivating Ifitm1 enhancing EV uptake [4]. In lung adenocarcinoma, IFITM1 is highly expressed through the activation of the STING-TBK1-IRF3 signaling in invasive cancer with phosphomimetic FoxM1, enhancing FoxM1-mediated signaling [3]. Also, in porcine epidemic diarrhea virus (PEDV) infection, overexpression of Ifitm1 suppresses PEDV proliferation in IPEC-J2 cells, while knockdown has the opposite effect [2].
In summary, Ifitm1 has diverse functions in both antiviral responses and tumor-related processes. Its role in promoting tumor progression and its impact on viral infections like PEDV highlight its significance in both oncology and virology research. Understanding Ifitm1 through functional studies, including gene knockout models, provides insights into disease mechanisms, potentially leading to new therapeutic strategies for cancer and viral diseases.
References:
1. Liang, Renba, Li, Xinxiao, Zhu, Xiaodong. . Deciphering the Roles of IFITM1 in Tumors. In Molecular diagnosis & therapy, 24, 433-441. doi:10.1007/s40291-020-00469-4. https://pubmed.ncbi.nlm.nih.gov/32394410/
2. Cheng, Jiahao, He, Jiayi, Feng, Simeng, Wang, Aibing, Yuan, Xiaomin. 2024. IFITM1 is a host restriction factor that inhibits porcine epidemic diarrhea virus infection. In Journal of nanobiotechnology, 22, 677. doi:10.1186/s12951-024-02884-9. https://pubmed.ncbi.nlm.nih.gov/39501328/
3. Xu, Rong, Lee, Young-Joo, Kim, Chang-Hyeon, Kim, Jung-Hyun, Yim, Hyungshin. 2023. Invasive FoxM1 phosphorylated by PLK1 induces the polarization of tumor-associated macrophages to promote immune escape and metastasis, amplified by IFITM1. In Journal of experimental & clinical cancer research : CR, 42, 302. doi:10.1186/s13046-023-02872-1. https://pubmed.ncbi.nlm.nih.gov/37968723/
4. Kelemen, Andrea, Carmi, Idan, Oszvald, Ádám, Buzás, Edit I, Wiener, Zoltán. 2021. IFITM1 expression determines extracellular vesicle uptake in colorectal cancer. In Cellular and molecular life sciences : CMLS, 78, 7009-7024. doi:10.1007/s00018-021-03949-w. https://pubmed.ncbi.nlm.nih.gov/34609520/
5. Yánez, Diana C, Ross, Susan, Crompton, Tessa. 2019. The IFITM protein family in adaptive immunity. In Immunology, 159, 365-372. doi:10.1111/imm.13163. https://pubmed.ncbi.nlm.nih.gov/31792954/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen