C57BL/6JCya-Ppt2em1/Cya
Common Name:
Ppt2-KO
Product ID:
S-KO-19115
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ppt2-KO
Strain ID
KOCMP-54397-Ppt2-B6J-VA
Gene Name
Product ID
S-KO-19115
Gene Alias
0610007M19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppt2em1/Cya mice (Catalog S-KO-19115) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000171376
NCBI RefSeq
NM_019441
Target Region
Exon 3~6
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Check the content of the review
Ppt2, or palmitoyl protein thioesterase 2, is a lysosomal thioesterase. It is highly associated with metabolism [1,2].
In fungi like Candida albicans, Ppt2 (phosphopantetheinyl transferase) transfers the phosphopantetheinyl group of coenzyme A to the acyl carrier protein Acp1 in mitochondria for lipoic acid synthesis essential for fungal respiration, making it a potential antifungal drug target [3,5].
In mouse models, disruption of Ppt2 causes an unusual lysosomal storage disorder with neurovisceral features. All Ppt2-deficient mice showed a neurodegenerative phenotype with spasticity and ataxia by 15 months, along with bone marrow infiltration, splenomegaly, and orange-brown pancreas due to lipofuscin pigment storage [4]. Ppt2-knockout mice developed spasticity at a median age of 29 weeks, and autofluorescent storage material was present in their brains [6].
In conclusion, Ppt2 is crucial for normal physiological function, especially in metabolism and lysosomal processes. Mouse knockout models have revealed its role in preventing a lysosomal storage disorder with neurovisceral manifestations. Understanding Ppt2 may provide insights into treating related neurodegenerative and fungal diseases.
References：
1. Xu, Hui, Zhang, Yan, Xie, Zhen, Zhao, Bei-Bei, Hua, Tian. 2024. Investigating PPT2's role in ovarian cancer prognosis and immunotherapy outcomes. In Journal of ovarian research, 17, 198. doi:10.1186/s13048-024-01527-9. https://pubmed.ncbi.nlm.nih.gov/39394143/
2. Yuan, ChangFei, Xiong, ZhiYong, Shi, Jian, Chen, Ke, Zhang, XiaoPing. 2020. Overexpression of PPT2 Represses the Clear Cell Renal Cell Carcinoma Progression by Reducing Epithelial-to-mesenchymal Transition. In Journal of Cancer, 11, 1151-1161. doi:10.7150/jca.36477. https://pubmed.ncbi.nlm.nih.gov/31956361/
3. Meng, Ling-Ning, Liu, Jin-Yan, Wang, Yu-Ting, Ni, Shuai-Shuai, Xiang, Ming-Jie. 2020. The discovery of potential phosphopantetheinyl transferase Ppt2 inhibitors against drug-resistant Candida albicans. In Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 51, 1665-1672. doi:10.1007/s42770-020-00318-w. https://pubmed.ncbi.nlm.nih.gov/32557281/
4. Gupta, Praveena, Soyombo, Abigail A, Shelton, John M, Richardson, James A, Hofmann, Sandra L. 2003. Disruption of PPT2 in mice causes an unusual lysosomal storage disorder with neurovisceral features. In Proceedings of the National Academy of Sciences of the United States of America, 100, 12325-30. doi:. https://pubmed.ncbi.nlm.nih.gov/14528005/
5. Dobb, Katharine S, Kaye, Sarah J, Beckmann, Nicola, Birch, Mike, Oliver, Jason D. 2015. Characterisation of the Candida albicans Phosphopantetheinyl Transferase Ppt2 as a Potential Antifungal Drug Target. In PloS one, 10, e0143770. doi:10.1371/journal.pone.0143770. https://pubmed.ncbi.nlm.nih.gov/26606674/
6. Gupta, P, Soyombo, A A, Atashband, A, Hammer, R E, Hofmann, S L. . Disruption of PPT1 or PPT2 causes neuronal ceroid lipofuscinosis in knockout mice. In Proceedings of the National Academy of Sciences of the United States of America, 98, 13566-71. doi:. https://pubmed.ncbi.nlm.nih.gov/11717424/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen