C57BL/6JCya-Mcm9em1/Cya
Common Name:
Mcm9-KO
Product ID:
S-KO-19139
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mcm9-KO
Strain ID
KOCMP-71567-Mcm9-B6J-VA
Gene Name
Product ID
S-KO-19139
Gene Alias
9030408O17Rik; Gm235; Mcmdc1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mcm9em1/Cya mice (Catalog S-KO-19139) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000237608
NCBI RefSeq
NM_027830
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Mcm9, also known as Minichromosome Maintenance 9 Homologous Recombination Repair Factor, is a key protein in multiple DNA-related processes. It is involved in DNA replication (such as the assembly of prereplication complexes), meiosis, homologous recombination, and DNA mismatch repair, which are crucial for maintaining genomic integrity [1,3,6].
Mutations in Mcm9 have been associated with several disorders. In humans, homozygous or compound heterozygous pathogenic variants in Mcm9 can lead to primary ovarian insufficiency (POI), Sertoli cell-only syndrome in males, and are also linked to early-onset cancer, mainly in a recessive inheritance pattern [2,4,5]. Mouse models with targeted mutations in genes related to the Mcm9-associated pathway, like Hrob (which recruits the MCM8-MCM9 helicase), result in infertility due to germ cell depletion and meiotic arrest, suggesting a role of the Mcm9-related pathway in gametogenesis [7].
In conclusion, Mcm9 is essential for DNA-related processes like replication and repair. Studies, including those using mouse models, have revealed its significant role in reproductive disorders such as POI and male infertility, as well as in early-onset cancer. Understanding Mcm9 provides insights into the mechanisms of these diseases and may offer potential diagnostic and therapeutic avenues.
References:
1. Helderman, Noah Cornelis, Terlouw, Diantha, Bonjoch, Laia, Goldberg, Yael, Nielsen, Maartje. 2023. Molecular functions of MCM8 and MCM9 and their associated pathologies. In iScience, 26, 106737. doi:10.1016/j.isci.2023.106737. https://pubmed.ncbi.nlm.nih.gov/37378315/
2. Goldberg, Yael, Aleme, Ola, Peled-Perets, Lilach, Nielsen, Maartje, Shalev, Stavit A. 2021. MCM9 is associated with germline predisposition to early-onset cancer-clinical evidence. In NPJ genomic medicine, 6, 78. doi:10.1038/s41525-021-00242-4. https://pubmed.ncbi.nlm.nih.gov/34556653/
3. Traver, Sabine, Coulombe, Philippe, Peiffer, Isabelle, Latreille, Daniel, Méchali, Marcel. 2015. MCM9 Is Required for Mammalian DNA Mismatch Repair. In Molecular cell, 59, 831-9. doi:10.1016/j.molcel.2015.07.010. https://pubmed.ncbi.nlm.nih.gov/26300262/
4. Potorac, Iulia, Laterre, Marie, Malaise, Olivier, Pétrossians, Patrick, Pintiaux, Axelle. 2023. The Role of MCM9 in the Etiology of Sertoli Cell-Only Syndrome and Premature Ovarian Insufficiency. In Journal of clinical medicine, 12, . doi:10.3390/jcm12030990. https://pubmed.ncbi.nlm.nih.gov/36769638/
5. Nauwynck, Elise, De Vos, Michel, Gheldof, Alexander, De Schepper, Jean, Staels, Willem. 2024. MCM9 compound heterozygosity in an adolescent with premature ovarian insufficiency. In Endocrinology, diabetes & metabolism case reports, 2024, . doi:10.1530/EDM-24-0012. https://pubmed.ncbi.nlm.nih.gov/39447595/
6. Lutzmann, Malik, Méchali, Marcel. . MCM9 binds Cdt1 and is required for the assembly of prereplication complexes. In Molecular cell, 31, 190-200. doi:10.1016/j.molcel.2008.07.001. https://pubmed.ncbi.nlm.nih.gov/18657502/
7. Hustedt, Nicole, Saito, Yuichiro, Zimmermann, Michal, Jurisicova, Andrea, Durocher, Daniel. 2019. Control of homologous recombination by the HROB-MCM8-MCM9 pathway. In Genes & development, 33, 1397-1415. doi:10.1101/gad.329508.119. https://pubmed.ncbi.nlm.nih.gov/31467087/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen