C57BL/6JCya-Gpr34em1/Cya
Common Name:
Gpr34-KO
Product ID:
S-KO-19199
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Gpr34-KO
Strain ID
KOCMP-23890-Gpr34-B6J-VB
Gene Name
Product ID
S-KO-19199
Gene Alias
Lypsr1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpr34em1/Cya mice (Catalog S-KO-19199) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041708
NCBI RefSeq
XM_006527624
Target Region
Exon 4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Gpr34, a G-protein-coupled receptor of Lysophosphatidylserine (LysoPS), is a key player in multiple biological processes. It is involved in immune cell function, tissue repair, and is associated with various diseases. The pathways it influences include PI3K-AKT and ERK signaling. Genetic models, like Gpr34-deficient mice, have been crucial in understanding its functions [1,2,3,4,5,6,7,8,9,10].
In the context of tissue repair, Gpr34-deficient mice exhibited compromised activation of type 3 innate lymphoid cells (ILC3s) and reduced tissue repair during colon injury, indicating that Gpr34 in ILC3s is essential for sensing apoptotic neutrophils and triggering tissue repair [2]. In neurodegenerative diseases, Gpr34 knockdown in APP/PS1 mice relieved cognitive deficits and suppressed neuroinflammation, suggesting its contribution to Alzheimer's disease pathogenesis [10]. In cancer, genetic deletion of Gpr34 in ILC1s enhanced their antitumor activity, highlighting Gpr34 as a metabolic immune checkpoint [4].
In conclusion, Gpr34 plays diverse and important roles in biological processes such as tissue repair, neuroinflammation, and antitumor immunity. Studies using Gpr34-deficient mouse models have significantly advanced our understanding of its functions in diseases like colon injury-related disorders, Alzheimer's disease, and cancer, offering potential therapeutic targets for these conditions.
References:
1. Xia, Anjie, Yong, Xihao, Zhang, Changbin, Shao, Zhenhua, Yang, Shengyong. 2023. Cryo-EM structures of human GPR34 enable the identification of selective antagonists. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2308435120. doi:10.1073/pnas.2308435120. https://pubmed.ncbi.nlm.nih.gov/37733739/
2. Wang, Xiaqiong, Cai, Juan, Lin, Bolong, Jiang, Wei, Zhou, Rongbin. . GPR34-mediated sensing of lysophosphatidylserine released by apoptotic neutrophils activates type 3 innate lymphoid cells to mediate tissue repair. In Immunity, 54, 1123-1136.e8. doi:10.1016/j.immuni.2021.05.007. https://pubmed.ncbi.nlm.nih.gov/34107271/
3. Lin, Bolong, Zhou, Yubo, Huang, Zonghui, Jiang, Wei, Zhou, Rongbin. 2024. GPR34 senses demyelination to promote neuroinflammation and pathologies. In Cellular & molecular immunology, 21, 1131-1144. doi:10.1038/s41423-024-01204-3. https://pubmed.ncbi.nlm.nih.gov/39030423/
4. Yan, Jiaxian, Zhang, Chi, Xu, Yueli, Jiang, Wei, Zhou, Rongbin. 2024. GPR34 is a metabolic immune checkpoint for ILC1-mediated antitumor immunity. In Nature immunology, 25, 2057-2067. doi:10.1038/s41590-024-01973-z. https://pubmed.ncbi.nlm.nih.gov/39358444/
5. Tam, Hanson, Xu, Ying, An, Jinping, Muppidi, Jagan R, Cyster, Jason G. 2024. Phosphatidylserine phospholipase A1 enables GPR34-dependent immune cell accumulation in the peritoneal cavity. In The Journal of experimental medicine, 221, . doi:10.1084/jem.20240992. https://pubmed.ncbi.nlm.nih.gov/39412501/
6. Schöneberg, Torsten, Meister, Jaroslawna, Knierim, Alexander Bernd, Schulz, Angela. 2018. The G protein-coupled receptor GPR34 - The past 20 years of a grownup. In Pharmacology & therapeutics, 189, 71-88. doi:10.1016/j.pharmthera.2018.04.008. https://pubmed.ncbi.nlm.nih.gov/29684466/
7. Korona, Boguslawa, Korona, Dagmara, Zhao, Wanfeng, Wotherspoon, Andrew C, Du, Ming-Qing. . GPR34 activation potentially bridges lymphoepithelial lesions to genesis of salivary gland MALT lymphoma. In Blood, 139, 2186-2197. doi:10.1182/blood.2020010495. https://pubmed.ncbi.nlm.nih.gov/34086889/
8. Sayo, Akira, Konishi, Hiroyuki, Kobayashi, Masaaki, Aoki, Junken, Kiyama, Hiroshi. 2019. GPR34 in spinal microglia exacerbates neuropathic pain in mice. In Journal of neuroinflammation, 16, 82. doi:10.1186/s12974-019-1458-8. https://pubmed.ncbi.nlm.nih.gov/30975169/
9. Chen, Lushu, Zhang, HuiYing, Zhang, Ying, Xu, Yong, Yao, Jin. 2024. Ganglion cell-derived LysoPS induces retinal neovascularisation by activating the microglial GPR34-PI3K-AKT-NINJ1 axis. In Journal of neuroinflammation, 21, 278. doi:10.1186/s12974-024-03265-7. https://pubmed.ncbi.nlm.nih.gov/39468551/
10. Lin, Lu-Lu, Song, Gui-Jun, Zhang, Hui, Ding, Li, Li, Yu. 2023. GPR34 Knockdown Relieves Cognitive Deficits and Suppresses Neuroinflammation in Alzheimer's Disease via the ERK/NF-κB Signal. In Neuroscience, 528, 129-139. doi:10.1016/j.neuroscience.2023.08.001. https://pubmed.ncbi.nlm.nih.gov/37557947/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen