C57BL/6JCya-Chst11em1/Cya
Common Name:
Chst11-KO
Product ID:
S-KO-19230
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Chst11-KO
Strain ID
KOCMP-58250-Chst11-B6J-VA
Gene Name
Product ID
S-KO-19230
Gene Alias
1110020P09Rik; C4ST; C4ST-1; C4ST1; C4s
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chst11em1/Cya mice (Catalog S-KO-19230) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040110
NCBI RefSeq
NM_021439
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Chst11, short for carbohydrate sulfotransferase 11, is a Golgi membrane protein that catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine residues of chondroitin. Chondroitin sulfate, whose sulfation is regulated by Chst11, is a key proteoglycan in cartilage, playing an important role in the growth plate development of cartilage [6]. It may also be involved in integrin signaling based on some analyses [2].
Chst11-deficient mice exhibit severe chondrodysplasia, congenital arthritis, and neonatal lethality, highlighting its crucial role in skeletal morphogenesis [6]. In human diseases, high expression of Chst11 is associated with poor prognosis in various cancers. For example, in glioblastoma, its overexpression promotes cell mobility and modulating chondroitin 4-sulfate on cells, and targeting Chst11-derived chondroitin 4-sulfate can attenuate cell invasiveness and improve survival of orthotopic glioma cell transplant mice [1]. In lung cancer, its expression contributes to tumor malignancy [2]. In pancreatic cancer, high expression correlates with poor prognosis and tumor immune infiltration [3]. In clear cell renal cell carcinoma, it promotes cell proliferation, migration, and invasion [7]. Also, in breast cancer, its expression is higher in basal-like and Her2-amplified cell lines and is associated with tumor progression, and its expression is controlled by DNA methylation [4]. In hepatocellular carcinoma cells, decreased or forced expression of Chst11 can alter metastatic potential and drug sensitivity, probably via the mitogen-activated protein kinase (MAPK) signal pathway [5].
In conclusion, Chst11 is essential for skeletal morphogenesis as demonstrated by mouse models. In disease research, its role in various cancers, such as promoting tumor progression and influencing prognosis, has been revealed through functional studies. Understanding Chst11's function provides potential targets for treating these diseases.
References:
1. Lin, You-Cheng, Chu, Yin-Hung, Liao, Wen-Chieh, Yang, Meng-Yin, Liu, Chiung-Hui. 2023. CHST11-modified chondroitin 4-sulfate as a potential therapeutic target for glioblastoma. In American journal of cancer research, 13, 2998-3012. doi:. https://pubmed.ncbi.nlm.nih.gov/37559985/
2. Li, Chien-Hsiu, Chan, Ming-Hsien, Chang, Yu-Chan, Hsiao, Michael. 2022. The CHST11 gene is linked to lung cancer and pulmonary fibrosis. In The journal of gene medicine, 24, e3451. doi:10.1002/jgm.3451. https://pubmed.ncbi.nlm.nih.gov/36181245/
3. Zhang, Pan, Chen, Dan, Cui, Haimeng, Luo, Qingfeng. . High Expression of CHST11 Correlates with Poor Prognosis and Tumor Immune Infiltration of Pancreatic Cancer. In Clinical laboratory, 68, . doi:10.7754/Clin.Lab.2022.211239. https://pubmed.ncbi.nlm.nih.gov/36546737/
4. Herman, Damir, Leakey, Tatiana I, Behrens, Alice, Kieber-Emmons, Thomas, Monzavi-Karbassi, Behjatolah. 2015. CHST11 gene expression and DNA methylation in breast cancer. In International journal of oncology, 46, 1243-51. doi:10.3892/ijo.2015.2828. https://pubmed.ncbi.nlm.nih.gov/25586191/
5. Zhou, Huimin, Li, Yanping, Song, Xiaobo, Zhao, Lifen, Jia, Li. 2016. CHST11/13 Regulate the Metastasis and Chemosensitivity of Human Hepatocellular Carcinoma Cells Via Mitogen-Activated Protein Kinase Pathway. In Digestive diseases and sciences, 61, 1972-85. doi:10.1007/s10620-016-4114-5. https://pubmed.ncbi.nlm.nih.gov/26993826/
6. Shabbir, Rana Muhammad Kamran, Nalbant, Gökhan, Ahmad, Nafees, Malik, Sajid, Tolun, Aslıhan. 2018. Homozygous CHST11 mutation in chondrodysplasia, brachydactyly, overriding digits, clino-symphalangism and synpolydactyly. In Journal of medical genetics, 55, 489-496. doi:10.1136/jmedgenet-2017-105003. https://pubmed.ncbi.nlm.nih.gov/29514872/
7. Hu, Weijing, Chen, Yongquan, Zhang, Lin, Wang, Dongwen, Wu, Bo. 2024. Effect of CHST11, a novel biomarker, on the biological functionalities of clear cell renal cell carcinoma. In Scientific reports, 14, 7704. doi:10.1038/s41598-024-58280-8. https://pubmed.ncbi.nlm.nih.gov/38565604/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen