C57BL/6JCya-Cdk7em1/Cya
Common Name:
Cdk7-KO
Product ID:
S-KO-19238
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cdk7-KO
Strain ID
KOCMP-12572-Cdk7-B6J-VA
Gene Name
Product ID
S-KO-19238
Gene Alias
C230069N13; CAK; Cdkn7; Crk4; P39 Mo15
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdk7em1/Cya mice (Catalog S-KO-19238) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000091299
NCBI RefSeq
NM_009874
Target Region
Exon 4~5
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Cdk7, also known as cyclin-dependent kinase 7, is a key kinase. It forms the CDK-activating complex (CAK) with cyclin H and MAT1, regulating cell cycle progression via T-loop phosphorylation of cell cycle CDKs. Additionally, as a component of the general transcription factor TFIIH, Cdk7-mediated phosphorylation of RNA polymerase II at active gene promoters enables transcription. It is thus crucial for both cell cycle and transcriptional processes [1,4,5,6,7].
In multiple myeloma, Cdk7 expression positively correlates with E2F and MYC transcriptional programs. Its inhibition counteracts E2F activity, impairs MYC-regulated metabolic gene signatures, and leads to in vivo tumor regression in mouse models [2]. In small cell lung cancer, inhibiting Cdk7 with YKL-5-124 disrupts cell-cycle progression, induces DNA replication stress and genome instability, and triggers an immune-response signaling, providing a survival benefit when combined with anti-PD-1 in murine models [3].
In conclusion, Cdk7 is essential for regulating cell cycle and gene transcription. Model-based research, especially in mouse models, has revealed its role in cancers such as multiple myeloma and small cell lung cancer. These findings highlight Cdk7 as a potential therapeutic target in oncology.
References:
1. Sava, Georgina P, Fan, Hailing, Coombes, R Charles, Buluwela, Lakjaya, Ali, Simak. . CDK7 inhibitors as anticancer drugs. In Cancer metastasis reviews, 39, 805-823. doi:10.1007/s10555-020-09885-8. https://pubmed.ncbi.nlm.nih.gov/32385714/
2. Yao, Yao, Ng, Jessica Fong, Park, Woojun Daniel, Munshi, Nikhil C, Fulciniti, Mariateresa. . CDK7 controls E2F- and MYC-driven proliferative and metabolic vulnerabilities in multiple myeloma. In Blood, 141, 2841-2852. doi:10.1182/blood.2022018885. https://pubmed.ncbi.nlm.nih.gov/36877894/
3. Zhang, Hua, Christensen, Camilla L, Dries, Ruben, Gray, Nathanael S, Wong, Kwok-Kin. 2019. CDK7 Inhibition Potentiates Genome Instability Triggering Anti-tumor Immunity in Small Cell Lung Cancer. In Cancer cell, 37, 37-54.e9. doi:10.1016/j.ccell.2019.11.003. https://pubmed.ncbi.nlm.nih.gov/31883968/
4. Gong, Ying, Li, Huiping. 2024. CDK7 in breast cancer: mechanisms of action and therapeutic potential. In Cell communication and signaling : CCS, 22, 226. doi:10.1186/s12964-024-01577-y. https://pubmed.ncbi.nlm.nih.gov/38605321/
5. Li, Zhi-Mei, Liu, Guan, Gao, Ya, Zhao, Ming-Gao. 2022. Targeting CDK7 in oncology: The avenue forward. In Pharmacology & therapeutics, 240, 108229. doi:10.1016/j.pharmthera.2022.108229. https://pubmed.ncbi.nlm.nih.gov/35700828/
6. Velychko, Taras, Mohammad, Eusra, Ferrer-Vicens, Ivan, Cramer, Patrick, Lidschreiber, Michael. 2024. CDK7 kinase activity promotes RNA polymerase II promoter escape by facilitating initiation factor release. In Molecular cell, 84, 2287-2303.e10. doi:10.1016/j.molcel.2024.05.007. https://pubmed.ncbi.nlm.nih.gov/38821049/
7. Fisher, Robert P. 2018. Cdk7: a kinase at the core of transcription and in the crosshairs of cancer drug discovery. In Transcription, 10, 47-56. doi:10.1080/21541264.2018.1553483. https://pubmed.ncbi.nlm.nih.gov/30488763/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen