C57BL/6JCya-Kcmf1em1/Cya
Common Name
Kcmf1-KO
Product ID
S-KO-19331
Backgroud
C57BL/6JCya
Strain ID
KOCMP-74287-Kcmf1-B6J-VB
When using this mouse strain in a publication, please cite “Kcmf1-KO Mouse (Catalog S-KO-19331) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Kcmf1-KO
Strain ID
KOCMP-74287-Kcmf1-B6J-VB
Gene Name
Product ID
S-KO-19331
Gene Alias
1700094M07Rik, Debt91, Pmcf
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000068697
NCBI RefSeq
NM_019715
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Overview of Gene Research
Kcmf1, or Potassium channel modulatory factor 1, is an E3 ubiquitin ligase. It plays a vital role in multiple biological processes, including renal tubulogenesis, preeclampsia, and tumor development in mammals. It is involved in ubiquitination-related pathways, which are crucial for protein degradation and regulation of various cellular functions [1,2,3,4,5].
In invertebrates like the mud crab (Scylla paramamosain), KCMF1-like was identified. RNA interference (RNAi) experiments, a form of in-vivo loss-of-function study, showed that knockdown of KCMF1-like increased the white spot syndrome virus (WSSV) copy number and impaired hemocytes apoptosis rate, indicating its role in defense against virus infection in the mud crab [1]. In patients with renal cell carcinoma (RCC), disruption in the networking of KCMF1-linked ubiquitin ligase impairs autophagy in CD8+ memory T cells, suggesting its potential as a therapeutic target [2]. Also, in a subset of epithelial tumors, a ubiquitin ligase complex containing Kcmf1 is required for survival, and suppressing related components can lead to tumor regression [3].
In conclusion, Kcmf1 is essential in various biological processes, especially those related to ubiquitination-mediated protein regulation. Studies using loss-of-function models, such as RNAi in invertebrates and disruption of its associated ligase in human diseases, have revealed its importance in antiviral defense in invertebrates and in tumor-related processes in mammals, highlighting its potential as a therapeutic target in cancer.
References：
1. Kong, Tongtong, Fang, Yameng, Fan, Xinyue, Li, Shengkang. 2023. KCMF1-like suppresses white spot syndrome virus infection by promoting apoptosis in mud crab (Scylla paramamosain). In Fish & shellfish immunology, 142, 109158. doi:10.1016/j.fsi.2023.109158. https://pubmed.ncbi.nlm.nih.gov/37832749/
2. Singh, Ashu, Choudhury, Saumitra Dey, Singh, Prabhjot, Kaushal, Seema, Sharma, Alpana. 2023. Disruption in networking of KCMF1 linked ubiquitin ligase impairs autophagy in CD8+ memory T cells of patients with renal cell carcinoma. In Cancer letters, 564, 216194. doi:10.1016/j.canlet.2023.216194. https://pubmed.ncbi.nlm.nih.gov/37084875/
3. Cervia, Lisa D, Shibue, Tsukasa, Borah, Ashir A, Vazquez, Francisca, Hahn, William C. . A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas. In Cancer discovery, 13, 766-795. doi:10.1158/2159-8290.CD-22-1230. https://pubmed.ncbi.nlm.nih.gov/36576405/
4. Singh, Ashu, Choudhury, Saumitra Dey, Singh, Prabhjot, Singh, Som Nath, Sharma, Alpana. 2022. KCMF1 regulates autophagy and ion channels' function in renal cell carcinoma: a future therapeutic target. In Journal of cancer research and clinical oncology, 149, 5617-5626. doi:10.1007/s00432-022-04507-y. https://pubmed.ncbi.nlm.nih.gov/36515749/
5. Hong, Jenny H, Kaustov, Lilia, Coyaud, Etienne, Arrowsmith, Cheryl, Raught, Brian. 2015. KCMF1 (potassium channel modulatory factor 1) Links RAD6 to UBR4 (ubiquitin N-recognin domain-containing E3 ligase 4) and lysosome-mediated degradation. In Molecular & cellular proteomics : MCP, 14, 674-85. doi:10.1074/mcp.M114.042168. https://pubmed.ncbi.nlm.nih.gov/25582440/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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