C57BL/6JCya-Rasd2em1/Cya
Common Name:
Rasd2-KO
Product ID:
S-KO-19470
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rasd2-KO
Strain ID
KOCMP-75141-Rasd2-B6J-VB
Gene Name
Product ID
S-KO-19470
Gene Alias
4930526B11Rik; Rhes; TEM-2; TEM2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rasd2em1/Cya mice (Catalog S-KO-19470) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000139848
NCBI RefSeq
NM_029182
Target Region
Exon 3
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Rasd2, encoding a Ras-related GTP-binding protein, is involved in multiple biological functions. It is associated with psychiatric disorders and has been shown to be expressed in various tissues like skin [1,4]. The Rhes/RASD2 GTPase complex is part of dopamine D1/D2 receptor-mediated signaling [6].
In UVM, silencing Rasd2 dampened cell growth, migration, and invasion both in vitro and in vivo, suggesting it promotes UVM development and metastasis via enhancing glycolysis [1]. In ovariectomized mice, Rasd2 overexpression in the hippocampus alleviated depression-like behaviors, and 9-hour fasting had antidepressant-like effects by upregulating Rasd2 and related proteins, blocked by DRD2 antagonists [2]. Also, in mice, 5-day unpredictable mild stress reduced Rasd2 expression, and its overexpression in the NAcc core alleviated stress-induced depression-like behaviors [3]. In thyroid cancer, genetic silencing of Rasd2 impaired malignant phenotypes, and miRNA-485-5p was identified as a negative regulator of Rasd2 [5].
In summary, Rasd2 plays significant roles in multiple disease conditions. In cancer, such as UVM and thyroid cancer, it promotes tumor-related processes. In psychiatric-related models, it is involved in depression-like behaviors. Gene knockout or knockdown models in mice have been crucial in revealing these functions, providing insights into potential therapeutic strategies for these diseases.
References:
1. Xie, Meng, Xin, Chun. 2022. RASD2 promotes the development and metastasis of uveal melanoma via enhancing glycolysis. In Biochemical and biophysical research communications, 610, 92-98. doi:10.1016/j.bbrc.2022.04.060. https://pubmed.ncbi.nlm.nih.gov/35461072/
2. Cheng, Ziqian, Zhang, Chaohe, Zhao, Fangyi, Cui, Ranji, Li, Bingjin. . Rasd2 Mediates Acute Fasting-Induced Antidepressant-Like Effects via Dopamine D2 Receptor Activation in Ovariectomized Mice. In The international journal of neuropsychopharmacology, 26, 217-229. doi:10.1093/ijnp/pyac082. https://pubmed.ncbi.nlm.nih.gov/36566472/
3. Cheng, Ziqian, Zhao, Fangyi, Piao, Jingjing, Cui, Ranji, Li, Bingjin. 2024. Rasd2 regulates depression-like behaviors via DRD2 neurons in the prelimbic cortex afferent to nucleus accumbens core circuit. In Molecular psychiatry, 30, 435-449. doi:10.1038/s41380-024-02684-5. https://pubmed.ncbi.nlm.nih.gov/39097664/
4. Vitucci, Daniela, Di Giorgio, Annabella, Napolitano, Francesco, Bertolino, Alessandro, Usiello, Alessandro. 2015. Rasd2 Modulates Prefronto-Striatal Phenotypes in Humans and 'Schizophrenia-Like Behaviors' in Mice. In Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 41, 916-27. doi:10.1038/npp.2015.228. https://pubmed.ncbi.nlm.nih.gov/26228524/
5. Li, Xiao-Yu, Sun, Jian-Ping, Guo, Hao, Yu, Na, Li, Qing-Huai. 2025. Modulation of RASD2 by miRNA-485-5p Drives Thyroid Cancer Progression and Metastasis. In The Kaohsiung journal of medical sciences, , e70028. doi:10.1002/kjm2.70028. https://pubmed.ncbi.nlm.nih.gov/40289764/
6. Bang, Sookhee, Steenstra, Catherine, Kim, Sangwon F. 2012. Striatum specific protein, Rhes regulates AKT pathway. In Neuroscience letters, 521, 142-7. doi:10.1016/j.neulet.2012.05.073. https://pubmed.ncbi.nlm.nih.gov/22683505/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen