C57BL/6JCya-Snx15em1/Cya
Common Name:
Snx15-KO
Product ID:
S-KO-19484
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Snx15-KO
Strain ID
KOCMP-69024-Snx15-B6J-VC
Gene Name
Product ID
S-KO-19484
Gene Alias
1500032B08Rik; E130013C21Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snx15em1/Cya mice (Catalog S-KO-19484) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025702
NCBI RefSeq
NM_026912
Target Region
Exon 2~3
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Snx15, a sorting nexin, is involved in protein trafficking and regulation of endosomal recycling pathways. It contains a Phox-homology domain crucial for protein-protein interactions among sorting nexins and other partners [1,5]. Sorting nexins are key regulators of the endosomal network, and Snx15 is known to be associated with clathrin-coated pits and vesicles, and it can bind to phosphatidylinositol 3-phosphate in early endosomes [3].
In a study of a variant APL case, a novel RARA-Snx15 fusion was detected, suggesting a potential role of Snx15 in the pathogenesis of APL [1]. In Alzheimer's disease research, overexpression of Snx15 in mouse neurons and astrocytes reduces Aβ production by increasing APP recycling to the cell surface, while down-regulation promotes Aβ production [2]. RNAi-mediated loss-of-function screens in human cells showed that suppression of Snx15 delays EGF receptor degradation due to a defect in vesicle movement into early endosomes [3]. Also, studies on IST1, an ESCRT-III protein, revealed that it interacts with Snx15, and together they contribute to specific endosomal recycling pathways [4,6]. Overexpression of Snx15 in COS-7 cells altered endosomal morphology and inhibited endocytosis and recycling of certain proteins [7].
In conclusion, Snx15 plays a crucial role in protein trafficking and endosomal recycling, which impacts various biological processes and disease conditions. Its involvement in APL through the RARA-Snx15 fusion and in Alzheimer's disease by regulating Aβ production showcases its significance in disease-related research. The findings from functional studies, such as RNAi-mediated knockdown and overexpression experiments, provide insights into its role in these biological processes and diseases.
References:
1. Hirade, Kentaro, Kusumoto, Shigeru, Abe, Akihiro, Iida, Shinsuke, Yamamoto, Yukiya. 2022. A novel RARA-SNX15 fusion in PML-RARA-positive acute promyelocytic leukemia with t(11;17;15)(q13;q21.2;q24.1). In International journal of hematology, 116, 956-960. doi:10.1007/s12185-022-03421-w. https://pubmed.ncbi.nlm.nih.gov/35854096/
2. Feng, Tuancheng, Niu, Mengmeng, Ji, Chengxiang, Xu, Huaxi, Zhang, Yun-Wu. 2015. SNX15 Regulates Cell Surface Recycling of APP and Aβ Generation. In Molecular neurobiology, 53, 3690-3701. doi:10.1007/s12035-015-9306-z. https://pubmed.ncbi.nlm.nih.gov/26115702/
3. Danson, Chris, Brown, Edward, Hemmings, Oliver J, Verkade, Paul, Cullen, Peter J. 2013. SNX15 links clathrin endocytosis to the PtdIns3P early endosome independently of the APPL1 endosome. In Journal of cell science, 126, 4885-99. doi:10.1242/jcs.125732. https://pubmed.ncbi.nlm.nih.gov/23986476/
4. Clippinger, Amy K, Naismith, Teresa V, Yoo, Wonjin, Kast, David J, Hanson, Phyllis I. 2023. IST1 regulates select recycling pathways. In Traffic (Copenhagen, Denmark), 25, e12921. doi:10.1111/tra.12921. https://pubmed.ncbi.nlm.nih.gov/37926552/
5. Phillips, S A, Barr, V A, Haft, D H, Taylor, S I, Haft, C R. 2000. Identification and characterization of SNX15, a novel sorting nexin involved in protein trafficking. In The Journal of biological chemistry, 276, 5074-84. doi:. https://pubmed.ncbi.nlm.nih.gov/11085978/
6. Clippinger, Amy K, Naismith, Teresa V, Yoo, Wonjin, Kast, David, Hanson, Phyllis I. 2023. IST1 regulates select endosomal recycling pathways. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.07.31.551359. https://pubmed.ncbi.nlm.nih.gov/37577466/
7. Barr, V A, Phillips, S A, Taylor, S I, Haft, C R. . Overexpression of a novel sorting nexin, SNX15, affects endosome morphology and protein trafficking. In Traffic (Copenhagen, Denmark), 1, 904-16. doi:. https://pubmed.ncbi.nlm.nih.gov/11208079/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen