C57BL/6JCya-Serpind1em1/Cya
Common Name:
Serpind1-KO
Product ID:
S-KO-20234
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Serpind1-KO
Strain ID
KOCMP-15160-Serpind1-B6J-VC
Gene Name
Product ID
S-KO-20234
Gene Alias
HC-II; HCII; Hcf2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Serpind1em1/Cya mice (Catalog S-KO-20234) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023450
NCBI RefSeq
NM_008223
Target Region
Exon 3~5
Size of Effective Region
~4.7 kb
Detailed Document
Overview of Gene Research
Serpind1, also known as heparin cofactor II, belongs to the serine protease inhibitor family. Some serpins, including Serpind1, utilize glycosaminoglycans (GAGs) to enhance the inhibition of target proteases, playing a role in modulating coagulation [3].
In epithelial ovarian cancer, Serpind1 expression is significantly elevated, and patients with higher expression have poor prognoses. It promotes the proliferation, migration, invasion, cell cycle transition, and epithelial-mesenchymal transition of ovarian cancer cells, while inhibiting apoptosis through promoting phosphorylation in the PI3K/AKT pathway. Inhibition of its expression leads to opposite effects, and adding a PI3K/AKT pathway inhibitor can reverse the promoting effect of Serpind1-overexpressing cells on the malignant biological behavior of ovarian cancer cells [1].
In papillary thyroid carcinoma, Serpind1 is identified as a biomarker related to SUMOylation, involved in immune-related pathways, with potential for targeted therapy [2].
In liver-related diseases, Serpind1 is among the differentially expressed proteins in plasma-derived extracellular vesicles of cirrhosis and hepatocellular carcinoma patients, potentially serving as a candidate biomarker for disease prognosis and diagnosis [4].
In glaucoma, Serpind1 shows significant upregulation and plays a vital role in glaucoma occurrences, associated with eye transparency decrease and glucose metabolism [5].
In systemic sclerosis-associated pulmonary hypertension, higher levels of Serpind1 are associated with better survival in patients [6].
In melanoma, Serpind1 in plasma-derived extracellular vesicles is relevant in differentiating between disease stages [7].
In intrahepatic cholestasis of pregnancy, Serpind1 expression is lower in patients, and it may serve as a non-invasive biomarker [8].
In atherosclerosis, polycyclic aromatic hydrocarbons accelerate arteriosclerosis by promoting miR-155 expression to downregulate Serpind1 [9].
In conclusion, Serpind1 has diverse functions, mainly as a serine protease inhibitor involved in coagulation-related processes. Its role in multiple diseases, such as cancers, glaucoma, and pregnancy-related diseases, has been revealed through various studies. Research on Serpind1 provides insights into disease mechanisms and potential biomarker and therapeutic target identification.
References：
1. Guo, Qian, Zhu, Liancheng, Wang, Caixia, Li, Xiao, Lin, Bei. 2019. SERPIND1 Affects the Malignant Biological Behavior of Epithelial Ovarian Cancer via the PI3K/AKT Pathway: A Mechanistic Study. In Frontiers in oncology, 9, 954. doi:10.3389/fonc.2019.00954. https://pubmed.ncbi.nlm.nih.gov/31637210/
2. Li, Xiang, Ding, Zigang, Tong, Yun. 2024. Identification of SUMOylation-related biomarkers in papillary thyroid carcinoma. In Cancer cell international, 24, 149. doi:10.1186/s12935-024-03323-3. https://pubmed.ncbi.nlm.nih.gov/38671425/
3. Rein, Chantelle M, Desai, Umesh R, Church, Frank C. . Serpin-glycosaminoglycan interactions. In Methods in enzymology, 501, 105-37. doi:10.1016/B978-0-12-385950-1.00007-9. https://pubmed.ncbi.nlm.nih.gov/22078533/
4. Zertuche-Martínez, Cecilia, Velázquez-Enríquez, Juan Manuel, González-García, Karina, Arellanes-Robledo, Jaime, Vásquez-Garzón, Verónica Rocío. 2024. Discovery of candidate biomarkers from plasma-derived extracellular vesicles of patients with cirrhosis and hepatocellular carcinoma: an exploratory proteomic study. In Molecular omics, 20, 483-495. doi:10.1039/d4mo00043a. https://pubmed.ncbi.nlm.nih.gov/39011654/
5. Liu, Xiaoyan, Liu, Xiang, Wang, Ying, Xu, Jiyu, Sun, Wei. 2021. Proteome Characterization of Glaucoma Aqueous Humor. In Molecular & cellular proteomics : MCP, 20, 100117. doi:10.1016/j.mcpro.2021.100117. https://pubmed.ncbi.nlm.nih.gov/34214668/
6. Mismetti, Valentine, Delavenne, Xavier, Montani, David, Bertoletti, Laurent, Cottin, Vincent. 2023. Proteomic biomarkers for survival in systemic sclerosis-associated pulmonary hypertension. In Respiratory research, 24, 273. doi:10.1186/s12931-023-02578-0. https://pubmed.ncbi.nlm.nih.gov/37936223/
7. Bollard, S M, Howard, J, Casalou, C, McCann, A, Potter, S M. 2024. Proteomic and metabolomic profiles of plasma-derived Extracellular Vesicles differentiate melanoma patients from healthy controls. In Translational oncology, 50, 102152. doi:10.1016/j.tranon.2024.102152. https://pubmed.ncbi.nlm.nih.gov/39405606/
8. Chen, Lingyan, Li, Jingyang, You, Yilan, Xiao, Jianping, Zhang, Yan. 2023. Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study. In Heliyon, 9, e21616. doi:10.1016/j.heliyon.2023.e21616. https://pubmed.ncbi.nlm.nih.gov/38027820/
9. He, Xiao-Nan, Xin, Jin-Yuan, Zhan, Jin-Liang, Zhang, Xiao-Ling, Bai, Ying-Chen. 2021. Polycyclic aromatic hydrocarbons induce endothelial injury through miR-155 to promote atherosclerosis. In Environmental and molecular mutagenesis, 62, 409-421. doi:10.1002/em.22454. https://pubmed.ncbi.nlm.nih.gov/34331478/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen