C57BL/6JCya-Mark2em1/Cya
Common Name
Mark2-KO
Product ID
S-KO-20296
Backgroud
C57BL/6JCya
Strain ID
KOCMP-13728-Mark2-B6J-VB
When using this mouse strain in a publication, please cite “Mark2-KO Mouse (Catalog S-KO-20296) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mark2-KO
Strain ID
KOCMP-13728-Mark2-B6J-VB
Gene Name
Product ID
S-KO-20296
Gene Alias
EMK-1, Emk, Par-1, Par-1b
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 19
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000165965
NCBI RefSeq
NM_007928
Target Region
Exon 4~14
Size of Effective Region
~5.2 kb
Overview of Gene Research
MARK2, also known as Microtubule affinity-regulating kinase 2, is a serine/threonine protein kinase. It phosphorylates microtubule-associated proteins and is crucial in multiple biological processes. MARK2 regulates signaling pathways like WNT, PI3K/AKT/mTOR, and NF-κB, linking it to cancer and the nervous system [3].
In neurodevelopment, MARK2 variants (predominantly loss-of-function) in 31 individuals were associated with autism spectrum disorder (ASD) and other neurodevelopmental disorders. MARK2 loss in proband-derived and CRISPR-engineered isogenic induced pluripotent stem cells led to early neuronal developmental and functional deficits. Mark2+/- mice showed abnormal cortical formation, partition, and ASD-like behavior, with MARK2 loss linked to down-regulation of the WNT/β-catenin signaling pathway [1]. In cancer, MARK2/3 were identified as catalytic codependencies of YAP/TAZ in human carcinoma and sarcoma. Inhibiting MARK2/3 can reactivate the Hippo tumor-suppressive pathway [2].
In conclusion, MARK2 is essential for neuronal development and is involved in cancer-related processes. Gene-knockout mouse models, such as Mark2+/- mice, have been instrumental in revealing its role in ASD-related neurodevelopmental disorders and cancer, providing insights into potential therapeutic targets for these diseases.
References：
1. Gong, Maolei, Li, Jiayi, Qin, Zailong, Liu, Chang-Mei, Chen, Xiaoli. 2024. MARK2 variants cause autism spectrum disorder via the downregulation of WNT/β-catenin signaling pathway. In American journal of human genetics, 111, 2392-2410. doi:10.1016/j.ajhg.2024.09.006. https://pubmed.ncbi.nlm.nih.gov/39419027/
2. Klingbeil, Olaf, Skopelitis, Damianos, Tonelli, Claudia, Cifani, Paolo, Vakoc, Christopher R. . MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer. In Cancer discovery, 14, 2471-2488. doi:10.1158/2159-8290.CD-23-1529. https://pubmed.ncbi.nlm.nih.gov/39058094/
3. Lei, Yining, Zhang, Ruyi, Cai, Fei. 2024. Role of MARK2 in the nervous system and cancer. In Cancer gene therapy, 31, 497-506. doi:10.1038/s41417-024-00737-z. https://pubmed.ncbi.nlm.nih.gov/38302729/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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