C57BL/6JCya-Cant1em1/Cya
Common Name:
Cant1-KO
Product ID:
S-KO-20389
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cant1-KO
Strain ID
KOCMP-76025-Cant1-B6J-VA
Gene Name
Product ID
S-KO-20389
Gene Alias
5830420C20Rik; Apy1h; D11Bwg0554e; Entpd8; SCAN-1; Shapy
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cant1em1/Cya mice (Catalog S-KO-20389) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000106289
NCBI RefSeq
NM_001267592
Target Region
Exon 3~4
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Cant1, also known as calcium-activated nucleotidase 1, is a phosphatase and a nucleotidase that preferentially hydrolyzes UDP to UMP and phosphate [3,4]. It is involved in glycosaminoglycan (GAG) synthesis and chondrocyte differentiation, and may play roles in multiple biological pathways such as cell cycle, DNA replication [1,2]. Gene knockout mouse models have been valuable in studying its functions [3].
In Cant1-knockout (KO) mice, which mimic Desbuquois dysplasia type 1, there are skeletal defects including short stature, thoracic kyphosis, and delta phalanx. The GAG content and extracellular matrix space are reduced in growth plate cartilage, and chondrocyte differentiation is impaired [3]. In addition, the synthesis and secretion of proteoglycans with shorter GAG chains occur in mutant mice, affecting the properties of decorin and aggrecan [4].
In conclusion, Cant1 is crucial for GAG synthesis and chondrocyte differentiation in cartilage. The Cant1 KO mouse model has provided insights into the pathogenesis of Desbuquois dysplasia type 1, highlighting the gene's importance in skeletal development and related diseases [3,4].
References：
1. Liu, Ting, Li, Zhi-Zhao, Sun, Lei, Zhou, Xin-Gang, Wang, Peng. 2023. Upregulated CANT1 is correlated with poor prognosis in hepatocellular carcinoma. In BMC cancer, 23, 1007. doi:10.1186/s12885-023-11463-4. https://pubmed.ncbi.nlm.nih.gov/37858061/
2. Yao, Qiwei, Yu, Yilin, Wang, Zhiping, Zheng, Qunhao, Li, Jiancheng. 2022. CANT1 serves as a potential prognostic factor for lung adenocarcinoma and promotes cell proliferation and invasion in vitro. In BMC cancer, 22, 117. doi:10.1186/s12885-022-09175-2. https://pubmed.ncbi.nlm.nih.gov/35090419/
3. Kodama, Kazuki, Takahashi, Hiroaki, Oiji, Nobuyasu, Ikegawa, Shiro, Furuichi, Tatsuya. 2020. CANT1 deficiency in a mouse model of Desbuquois dysplasia impairs glycosaminoglycan synthesis and chondrocyte differentiation in growth plate cartilage. In FEBS open bio, 10, 1096-1103. doi:10.1002/2211-5463.12859. https://pubmed.ncbi.nlm.nih.gov/32277574/
4. Gramegna Tota, Chiara, Leone, Alessandra, Khan, Asifa, Rossi, Antonio, Paganini, Chiara. 2024. Cant1 Affects Cartilage Proteoglycan Properties: Aggrecan and Decorin Characterization in a Mouse Model of Desbuquois Dysplasia Type 1. In Biomolecules, 14, . doi:10.3390/biom14091064. https://pubmed.ncbi.nlm.nih.gov/39334831/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen