C57BL/6JCya-Dhx16em1/Cya
Common Name
Dhx16-KO
Product ID
S-KO-20405
Backgroud
C57BL/6JCya
Strain ID
KOCMP-69192-Dhx16-B6J-VB
When using this mouse strain in a publication, please cite “Dhx16-KO Mouse (Catalog S-KO-20405) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Dhx16-KO
Strain ID
KOCMP-69192-Dhx16-B6J-VB
Gene Name
Product ID
S-KO-20405
Gene Alias
2410006N22Rik, DBP2, Ddx16, mKIAA0577
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000025292
NCBI RefSeq
NM_026987
Target Region
Exon 8~9
Size of Effective Region
~1.1 kb
Overview of Gene Research
Dhx16, also known as DBP2 (DEAD-box protein 2), is an ATP-dependent RNA helicase and a key spliceosome component. It plays a vital role in processes like pre-mRNA splicing, ribosome assembly, and innate antiviral immunity [1,2,3,4,5]. In pre-mRNA splicing, it functions after the formation of a pre-catalytic spliceosome, similar to the role of Saccharomyces cerevisiae spliceosomal ATPase Prp2 [3].
Using conditional knockout (CKO) mice, it was demonstrated that loss of Dhx16 in the hematopoietic system leads to significant depletion of hematopoietic stem and progenitor cells, bone marrow failure, and rapid mortality. Dhx16-deficient HSCs show impaired quiescence, cell cycle arrest, reduced protein synthesis, abnormal ribosome assembly, increased apoptosis, and decreased self-renewal capacity [2]. In addition, silencing of Dhx16 in cells and in vivo diminished type I interferon (IFN-I) responses against influenza virus, Zika virus, and SARS-CoV-2. Dhx16-dependent IFN-I production requires RIG-I and unanchored K48-poly-Ub synthesized by the E3-Ub ligase TRIM6 [1].
In conclusion, Dhx16 is crucial for processes such as pre-mRNA splicing, ribosome assembly, and innate antiviral immunity. Studies using CKO mouse models have revealed its significance in maintaining hematopoietic stem cell homeostasis and in antiviral defense. These findings contribute to our understanding of hematopoietic diseases and antiviral immunity, potentially providing insights for therapeutic strategies.
References：
1. Hage, Adam, Bharaj, Preeti, van Tol, Sarah, Freiberg, Alexander N, Rajsbaum, Ricardo. . The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity. In Cell reports, 38, 110434. doi:10.1016/j.celrep.2022.110434. https://pubmed.ncbi.nlm.nih.gov/35263596/
2. Li, Zhigang, Fan, Jiankun, Xiao, Yalan, Zhao, Xueya, Hou, Yu. 2024. Essential role of Dhx16-mediated ribosome assembly in maintenance of hematopoietic stem cells. In Leukemia, 38, 2699-2708. doi:10.1038/s41375-024-02423-3. https://pubmed.ncbi.nlm.nih.gov/39333759/
3. Gencheva, Marieta, Kato, Mitsuo, Newo, Alain N S, Lin, Ren-Jang. . Contribution of DEAH-box protein DHX16 in human pre-mRNA splicing. In The Biochemical journal, 429, 25-32. doi:10.1042/BJ20100266. https://pubmed.ncbi.nlm.nih.gov/20423332/
4. Schmitzová, Jana, Cretu, Constantin, Dienemann, Christian, Urlaub, Henning, Pena, Vladimir. 2023. Structural basis of catalytic activation in human splicing. In Nature, 617, 842-850. doi:10.1038/s41586-023-06049-w. https://pubmed.ncbi.nlm.nih.gov/37165190/
5. Gencheva, Marieta, Lin, Ting-Yu, Wu, Xiwei, Lin, Shwu-Bin, Lin, Ren-Jang. 2010. Nuclear retention of unspliced pre-mRNAs by mutant DHX16/hPRP2, a spliceosomal DEAH-box protein. In The Journal of biological chemistry, 285, 35624-32. doi:10.1074/jbc.M110.122309. https://pubmed.ncbi.nlm.nih.gov/20841358/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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